################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## Acc65I AccI AccII AccIII AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaWI BseAI BsgI BsiEI BsiWI BsmFI BsmI BsoBI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HaeIII HgaI HhaI HinP1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NciI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 39 GACGTC GACGT^C AclI 482 AACGTT AA^CGTT AcyI 39 GACGTC G[GA]^CG[CT]C Asp700I 1583 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 200 GTGCTC G[AT]GC[AT]^C AvaII 186 GGACC G^G[AT]CC AvrII 493 CCTAGG C^CTAGG BanII 1563 GAGCCC G[GA]GC[CT]^C BciVI 388 GGATAC GTATCC(6/5) BlnI 493 CCTAGG C^CTAGG BpmI 776 CTCCAG CTGGAG(16/14) BsaBI 466 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 39 GACGTC G[GA]^CG[CT]C BsaI 1456 GAGACC GGTCTC(1/5) BsiHKAI 200 GTGCTC G[AT]GC[AT]^C BsmBI 37 GAGACG CGTCTC(1/5) Bsp1407I 1231 TGTACA T^GTACA BspDI 845 ATCGAT AT^CGAT BspMI 61 GCAGGT ACCTGC(4/8) BsrDI 923 GCAATG GCAATG(2/0) BsrGI 1231 TGTACA T^GTACA BssKI 356 CCAGG ^CC[ACGT]GG BstNI 356 CCAGG CC^[AT]GG BstYI 742 GGATCT [GA]^GATC[CT] Cfr13I 186 GGACC G^G[ACGT]CC ClaI 845 ATCGAT AT^CGAT DraI 1123 TTTAAA TTT^AAA DsaI 1430 CCATGG C^C[GA][CT]GG EcoRII 356 CCAGG ^CC[AT]GG FauI 692 GCGGG CCCGC(4/6) Hin1I 39 GACGTC G[GA]^CG[CT]C HindIII 1585 AAGCTT A^AGCTT MamI 466 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 742 GGATCT [GA]^GATC[CT] MspA1I 1757 CAGCTG C[AC]G^C[GT]G MvaI 356 CCAGG CC^[AT]GG NcoI 1430 CCATGG C^CATGG NheI 88 GCTAGC G^CTAGC NspI 1378 GCATGC [GA]CATG^[CT] Psp1406I 482 AACGTT AA^CGTT PstI 59 CTGCAG CTGCA^G PvuII 1757 CAGCTG CAG^CTG Sau96I 186 GGACC G^G[ACGT]CC ScrFI 356 CCAGG CC^[ACGT]GG SexAI 355 ACCAGGT A^CC[AT]GGT SphI 1378 GCATGC GCATG^C SspBI 1231 TGTACA T^GTACA SspI 1522 AATATT AAT^ATT XhoII 742 GGATCT [GA]^GATC[CT] XmnI 1583 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BsaJI 493 CCTAGG C^C[ACGT][ACGT]GG 1430 CCATGG BstXI 189 CCATAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1736 CCAGTAGTTTGG Cac8I 88 GCTAGC GC[ACGT]^[ACGT]GC 1378 GCATGC EarI 979 CTCTTC CTCTTC(1/4) 1560 GAAGAG EcoT14I 493 CCTAGG C^C[AT][AT]GG 1430 CCATGG HapII 223 CCGG C^CGG 534 CCGG HpaII 223 CCGG C^CGG 534 CCGG Ksp632I 979 CTCTTC CTCTTC(1/4) 1560 GAAGAG MspI 223 CCGG C^CGG 534 CCGG NdeI 363 CATATG CA^TATG 1768 CATATG SapI 1560 GAAGAGC GCTCTTC(1/4) 978 GCTCTTC SfaNI 1054 GATGC GCATC(5/9) 1186 GATGC SfcI 59 CTGCAG C^T[GA][CT]AG 992 CTGTAG StyI 493 CCTAGG C^C[AT][AT]GG 1430 CCATGG TaqI 846 TCGA T^CGA 1212 TCGA Tsp45I 404 GTGAC ^GT[GC]AC 1753 GTGAC TthHB8I 846 TCGA T^CGA 1212 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BfaI BmyI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BstF5I DdeI DpnII Fnu4HI FokI HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV RsaI Sau3AI TfiI Tru9I TseI Tsp509I TspRI