(Circular) (Six-base) MAPSORT of: pFN21AE5739.seq Check: 3913 from: 1 to: 6532 pFN21AE5739 6532 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2048 4342 278 Size: 53 83 186 936 512 2294 2468 Fragments arranged by size: 2468 2294 936 512 186 83 53 AccI GT'mk_AC Cuts at: 1937 3875 1937 Size: 1938 4594 Acc65I G'GTAC_C Cuts at: 3853 3853 Size: 6532 AclI AA'CG_TT Cuts at: 1563 2488 4659 5032 1563 Size: 925 2171 373 3063 Fragments arranged by size: 3063 2171 925 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4595 5979 6247 1632 Size: 2963 1384 268 1917 Fragments arranged by size: 2963 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6335 AflIII A'CryG_T Cuts at: 1203 5431 6408 1203 Size: 4228 977 1327 Fragments arranged by size: 4228 1327 977 AgeI A'CCGG_T Cuts at: 5363 5363 Size: 6532 AhdI GACnn_n'nnGTC Cuts at: 5261 5261 Size: 6532 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6532 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6532 AlwNI CAG_nnn'CTG Cuts at: 4275 5847 4275 Size: 1572 4960 ApaLI G'TGCA_C Cuts at: 4589 5745 4589 Size: 1156 5376 ApoI r'AATT_y Cuts at: 1493 2503 2812 3027 3164 3264 3841 4067 4219 Size: 1010 309 215 137 100 577 226 152 Cuts at: 4219 6328 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1010 577 309 226 215 152 137 100 AseI AT'TA_AT Cuts at: 160 1032 5086 160 Size: 872 4054 1606 Fragments arranged by size: 4054 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 AvaI C'yCGr_G Cuts at: 1870 1943 3857 1870 Size: 73 1914 4545 Fragments arranged by size: 4545 1914 73 AvrII C'CTAG_G Cuts at: 2498 2498 Size: 6532 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2340 2373 2340 Size: 33 6499 BamHI G'GATC_C Cuts at: 3862 4229 3862 Size: 367 6165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3853 5308 618 Size: 325 217 627 2066 1455 1842 Fragments arranged by size: 2066 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3572 3851 1391 Size: 2181 279 4072 Fragments arranged by size: 4072 2181 279 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5561 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4723 4757 1903 Size: 34 2786 34 3678 Fragments arranged by size: 3678 2786 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2387 4424 5634 2387 Size: 2037 1210 3285 Fragments arranged by size: 3285 2037 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6532 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5143 136 Size: 107 122 71 631 605 3471 1525 Fragments arranged by size: 3471 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6527 6527 Size: 6532 BlpI GC'TnA_GC Cuts at: 3949 3949 Size: 6532 Bme1580I G_kGCm'C Cuts at: 1790 3410 4593 5749 1790 Size: 1620 1183 1156 2573 Fragments arranged by size: 2573 1620 1183 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6532 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4263 5221 442 Size: 416 53 83 399 24 2846 958 1753 Fragments arranged by size: 2846 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2097 1056 Size: 1041 5491 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2766 5192 1239 Size: 1527 2426 2579 Fragments arranged by size: 2579 2426 1527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4657 5522 5820 4657 Size: 865 298 5369 Fragments arranged by size: 5369 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3455 5195 882 Size: 617 1956 1740 2219 Fragments arranged by size: 2219 1956 1740 617 BsaAI yAC'GTr Cuts at: 493 1204 4323 493 Size: 711 3119 2702 Fragments arranged by size: 3119 2702 711 BsaBI GATnn'nnATC Cuts at: 2475 6526 2475 Size: 4051 2481 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 4339 4721 5356 Size: 53 83 186 936 512 2294 382 635 Cuts at: 5356 275 Size: 1451 Fragments arranged by size: 2294 1451 936 635 512 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4965 5363 5637 5784 6195 4965 Size: 398 274 147 411 5302 Fragments arranged by size: 5302 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6307 Fragments arranged by size: 6307 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2205 2229 3710 1186 Size: 1019 24 1481 4008 Fragments arranged by size: 4008 1481 1019 24 BseYI C'CCAG_C Cuts at: 5735 6231 5735 Size: 496 6036 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5959 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3982 4743 5771 1540 Size: 275 186 1981 761 1028 2301 Fragments arranged by size: 2301 1981 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2209 4593 4678 5749 2209 Size: 2384 85 1071 2992 Fragments arranged by size: 2992 2384 1071 85 BsmI GAATG_Cn' Cuts at: 4043 4136 4043 Size: 93 6439 BsmBI CGTCTCn'nnnn_ Cuts at: 2036 2036 Size: 6532 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2209 3410 3572 3851 4593 4678 5749 Size: 399 419 1201 162 279 742 85 1071 Cuts at: 5749 1391 Size: 2174 Fragments arranged by size: 2174 1201 1071 742 419 399 279 162 85 BspEI T'CCGG_A Cuts at: 6195 6195 Size: 6532 BspHI T'CATG_A Cuts at: 4421 6151 4421 Size: 1730 4802 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 3887 844 Size: 1213 1830 3489 Fragments arranged by size: 3489 1830 1213 BsrBI CCG'CTC Cuts at: 1394 1823 4419 1394 Size: 429 2596 3507 Fragments arranged by size: 3507 2596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2935 5027 5201 65 Size: 1183 1687 2092 174 1396 Fragments arranged by size: 2092 1687 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5176 5363 1700 Size: 110 3366 187 2869 Fragments arranged by size: 3366 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3236 96 Size: 3140 3392 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6532 BssSI C'ACGA_G Cuts at: 4592 5604 4592 Size: 1012 5520 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6532 BstBI TT'CG_AA Cuts at: 5336 5336 Size: 6532 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6532 BstXI CCAn_nnnn'nTGG Cuts at: 2201 3748 2201 Size: 1547 4985 BstYI r'GATC_y Cuts at: 1972 2747 3862 4229 4614 4631 6083 6169 6181 Size: 775 1115 367 385 17 1452 86 12 Cuts at: 6181 6527 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1115 775 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3435 513 Size: 552 2370 3610 Fragments arranged by size: 3610 2370 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5793 Fragments arranged by size: 5793 683 56 BtsI GCAGTG_nn' Cuts at: 2529 4048 4842 4862 2529 Size: 1519 794 20 4199 Fragments arranged by size: 4199 1519 794 20 ClaI AT'CG_AT Cuts at: 1554 2851 1554 Size: 1297 5235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6497 DraI TTT'AAA Cuts at: 3130 3836 3130 Size: 706 5826 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5539 809 Size: 507 4223 1802 Fragments arranged by size: 4223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3979 4868 6497 Size: 54 1425 50 275 18 2149 889 1629 Cuts at: 6497 8 Size: 43 Fragments arranged by size: 2149 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3979 1537 Size: 275 2167 4090 Fragments arranged by size: 4090 2167 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2990 3560 4462 1353 Size: 1637 570 902 3423 Fragments arranged by size: 3423 1637 902 570 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5119 5493 5639 387 Size: 1064 138 3530 374 146 1280 Fragments arranged by size: 3530 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2766 4595 5192 5979 6247 1239 Size: 393 1134 1829 597 787 268 1524 Fragments arranged by size: 1829 1524 1134 787 597 393 268 EcoRI G'AATT_C Cuts at: 3841 4219 3841 Size: 378 6154 FspI TGC'GCA Cuts at: 5038 5038 Size: 6532 HaeII r_GCGC'y Cuts at: 1678 5679 1678 Size: 4001 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2385 2417 2783 2815 3751 Size: 32 135 32 813 32 366 32 936 Cuts at: 3751 3783 5173 5205 5247 5279 1373 Size: 32 1390 32 42 32 2626 Fragments arranged by size: 2626 1390 936 813 366 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3876 4122 669 Size: 988 281 1938 246 3079 Fragments arranged by size: 3079 1938 988 281 246 HindIII A'AGCT_T Cuts at: 748 3590 748 Size: 2842 3690 HpaI GTT'AAC Cuts at: 4122 4122 Size: 6532 KpnI G_GTAC'C Cuts at: 3857 3857 Size: 6532 MfeI C'AATT_G Cuts at: 4131 4131 Size: 6532 MluI A'CGCG_T Cuts at: 6408 6408 Size: 6532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 2243 2657 5646 5830 1305 Size: 837 101 414 2989 184 2007 Fragments arranged by size: 2989 2007 837 414 184 101 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4710 Fragments arranged by size: 4710 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2199 2388 2479 4490 4849 5008 518 Size: 648 1033 189 91 2011 359 159 2042 Fragments arranged by size: 2042 2011 1033 648 359 189 159 91 MspA1I CmG'CkG Cuts at: 1606 3764 3948 4625 5773 6018 1606 Size: 2158 184 677 1148 245 2120 Fragments arranged by size: 2158 2120 1148 677 245 184 NcoI C'CATG_G Cuts at: 513 1065 3435 513 Size: 552 2370 3610 Fragments arranged by size: 3610 2370 552 NdeI CA'TA_TG Cuts at: 387 2369 3774 387 Size: 1982 1405 3145 Fragments arranged by size: 3145 1982 1405 NheI G'CTAG_C Cuts at: 1052 2093 1052 Size: 1041 5491 NotI GC'GGCC_GC Cuts at: 3979 3979 Size: 6532 NspI r_CATG'y Cuts at: 1132 1229 3387 3890 5435 1132 Size: 97 2158 503 1545 2229 Fragments arranged by size: 2229 2158 1545 503 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6532 PciI A'CATG_T Cuts at: 5431 5431 Size: 6532 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6532 PmeI GTTT'AAAC Cuts at: 3836 3836 Size: 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4601 4633 6114 6146 4601 Size: 32 1481 32 4987 Fragments arranged by size: 4987 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6532 PsiI TTA'TAA Cuts at: 4102 4102 Size: 6532 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3313 3345 3313 Size: 32 6500 PstI C_TGCA'G Cuts at: 830 2068 3884 830 Size: 1238 1816 3478 Fragments arranged by size: 3478 1816 1238 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6532 PvuII CAG'CTG Cuts at: 3764 3764 Size: 6532 SalI G'TCGA_C Cuts at: 1936 3874 1936 Size: 1938 4594 SapI GCTCTTCn'nnn_ Cuts at: 2990 3560 2990 Size: 570 5962 SbfI CC_TGCA'GG Cuts at: 3884 3884 Size: 6532 ScaI AGT'ACT Cuts at: 4780 6492 4780 Size: 1712 4820 SexAI A'CCwGG_T Cuts at: 2360 2360 Size: 6532 SfcI C'TryA_G Cuts at: 826 1045 2064 2997 3880 5015 5696 5887 826 Size: 219 1019 933 883 1135 681 191 1471 Fragments arranged by size: 1471 1135 1019 933 883 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6532 SmaI CCC'GGG Cuts at: 1872 3859 1872 Size: 1987 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4636 5537 5799 820 Size: 197 926 2693 901 262 1553 Fragments arranged by size: 2693 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4323 493 Size: 3830 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6532 SphI G_CATG'C Cuts at: 1132 3387 3890 1132 Size: 2255 503 3774 Fragments arranged by size: 3774 2255 503 SspI AAT'ATT Cuts at: 5 52 3529 4456 5 Size: 47 3477 927 2081 Fragments arranged by size: 3477 2081 927 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6532 StyI C'CwwG_G Cuts at: 513 1065 2498 3435 3971 513 Size: 552 1433 937 536 3074 Fragments arranged by size: 3074 1433 937 552 536 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3256 4559 4576 4729 4914 5359 325 Size: 1255 1676 1303 17 153 185 445 1498 Fragments arranged by size: 1676 1498 1303 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2877 3236 3366 Size: 274 80 33 51 1443 900 359 130 Cuts at: 3366 4778 6490 96 Size: 1412 1712 138 Fragments arranged by size: 1712 1443 1412 900 359 274 138 130 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6492 6524 6492 Size: 32 6500 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6532 XbaI T'CTAG_A Cuts at: 3868 3868 Size: 6532 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6532 XmaI C'CCGG_G Cuts at: 1870 3857 1870 Size: 1987 4545 XmnI GAAnn'nnTTC Cuts at: 3592 4661 3592 Size: 1069 5463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2046 4340 276 Size: 53 83 186 936 512 2294 2468 Fragments arranged by size: 2468 2294 936 512 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI