(Linear) (Six-base) MAPSORT of: KIEE5739.seq Check: 2575 from: 1 to: 1827 KIEE5739.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AatII G_ACGT'C Cuts at: 0 43 1827 Size: 43 1784 AclI AA'CG_TT Cuts at: 0 483 1827 Size: 483 1344 ApoI r'AATT_y Cuts at: 0 498 807 1022 1159 1259 1827 Size: 498 309 215 137 100 568 Fragments arranged by size: 568 498 309 215 137 100 AvrII C'CTAG_G Cuts at: 0 493 1827 Size: 493 1334 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 335 368 1827 Size: 335 33 1459 Fragments arranged by size: 1459 335 33 BanII G_rGCy'C Cuts at: 0 1567 1827 Size: 1567 260 BciVI GTATCCnnnnn_n' Cuts at: 0 382 1827 Size: 382 1445 Bme1580I G_kGCm'C Cuts at: 0 1405 1827 Size: 1405 422 BmtI G_CTAG'C Cuts at: 0 92 1827 Size: 92 1735 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 761 1827 Size: 761 1066 BsaI GGTCTCn'nnnn_ Cuts at: 0 1450 1827 Size: 1450 377 BsaBI GATnn'nnATC Cuts at: 0 470 1827 Size: 470 1357 BsaHI Gr'CG_yC Cuts at: 0 40 1827 Size: 40 1787 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 200 224 1705 1827 Size: 200 24 1481 122 Fragments arranged by size: 1481 200 122 24 BsiHKAI G_wGCw'C Cuts at: 0 204 1827 Size: 204 1623 BsmBI CGTCTCn'nnnn_ Cuts at: 0 31 1827 Size: 31 1796 Bsp1286I G_dGCh'C Cuts at: 0 204 1405 1567 1827 Size: 204 1201 162 260 Fragments arranged by size: 1201 260 204 162 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 52 1827 Size: 52 1775 BsrDI GCAATG_nn' Cuts at: 0 930 1827 Size: 930 897 BsrGI T'GTAC_A Cuts at: 0 1231 1827 Size: 1231 596 BstXI CCAn_nnnn'nTGG Cuts at: 0 196 1743 1827 Size: 196 1547 84 Fragments arranged by size: 1547 196 84 BstYI r'GATC_y Cuts at: 0 742 1827 Size: 742 1085 BtgI C'CryG_G Cuts at: 0 1430 1827 Size: 1430 397 BtsI GCAGTG_nn' Cuts at: 0 524 1827 Size: 524 1303 ClaI AT'CG_AT Cuts at: 0 846 1827 Size: 846 981 DraI TTT'AAA Cuts at: 0 1125 1827 Size: 1125 702 EarI CTCTTCn'nnn_ Cuts at: 0 985 1555 1827 Size: 985 570 272 Fragments arranged by size: 985 570 272 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 761 1827 Size: 761 1066 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 380 412 778 810 1746 1778 1827 Size: 380 32 366 32 936 32 49 Fragments arranged by size: 936 380 366 49 32 32 32 HindIII A'AGCT_T Cuts at: 0 1585 1827 Size: 1585 242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 137 238 652 1827 Size: 137 101 414 1175 Fragments arranged by size: 1175 414 137 101 MslI CAynn'nnrTG Cuts at: 0 194 383 474 1827 Size: 194 189 91 1353 Fragments arranged by size: 1353 194 189 91 MspA1I CmG'CkG Cuts at: 0 1759 1827 Size: 1759 68 NcoI C'CATG_G Cuts at: 0 1430 1827 Size: 1430 397 NdeI CA'TA_TG Cuts at: 0 364 1769 1827 Size: 364 1405 58 Fragments arranged by size: 1405 364 58 NheI G'CTAG_C Cuts at: 0 88 1827 Size: 88 1739 NspI r_CATG'y Cuts at: 0 1382 1827 Size: 1382 445 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1308 1340 1827 Size: 1308 32 487 Fragments arranged by size: 1308 487 32 PstI C_TGCA'G Cuts at: 0 63 1827 Size: 63 1764 PvuII CAG'CTG Cuts at: 0 1759 1827 Size: 1759 68 SapI GCTCTTCn'nnn_ Cuts at: 0 985 1555 1827 Size: 985 570 272 Fragments arranged by size: 985 570 272 SexAI A'CCwGG_T Cuts at: 0 355 1827 Size: 355 1472 SfcI C'TryA_G Cuts at: 0 59 992 1827 Size: 59 933 835 Fragments arranged by size: 933 835 59 SphI G_CATG'C Cuts at: 0 1382 1827 Size: 1382 445 SspI AAT'ATT Cuts at: 0 1524 1827 Size: 1524 303 StyI C'CwwG_G Cuts at: 0 493 1430 1827 Size: 493 937 397 Fragments arranged by size: 937 493 397 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1251 1827 Size: 1251 576 TatI w'GTAC_w Cuts at: 0 872 1231 1361 1827 Size: 872 359 130 466 Fragments arranged by size: 872 466 359 130 XmnI GAAnn'nnTTC Cuts at: 0 1587 1827 Size: 1587 240 ZraI GAC'GTC Cuts at: 0 41 1827 Size: 41 1786 Enzymes that do cut: AatII AclI ApoI AvrII BaeI BanII BciVI Bme1580I BmtI BpmI BsaI BsaBI BsaHI BseRI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI BstXI BstYI BtgI BtsI ClaI DraI EarI Eco57MI Hin4I HindIII MmeI MslI MspA1I NcoI NdeI NheI NspI PsrI PstI PvuII SapI SexAI SfcI SphI SspI StyI TaqII TatI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI BamHI BanI BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI Bpu10I BpuEI BsaAI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI