##################################
# NO CUT ENZYMES (  228 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FokI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MfeI        MluI        MroI        MslI        MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        TseI        Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AluI          113  AGCT                 AG^CT
BalI          138  TGGCCA               TGG^CCA
BamHI          29  GGATCC               G^GATCC
BsiYI          20  CCTTAAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           20  CCTTAAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
EaeI          138  TGGCCA               [CT]^GGCC[GA]
EcoNI          20  CCTTAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeIII        139  GGCC                 GG^CC
HinfI          36  GAGTC                G^A[ACGT]TC
MaeIII         91  GTGAC                ^GT[ACGT]AC
MboII          76  GAAGA                GAAGA(8/7)
MluNI         138  TGGCCA               TGG^CCA
MscI          138  TGGCCA               TGG^CCA
MseI           22  TTAA                 T^TAA
MspA1I        112  CAGCTG               C[AC]G^C[GT]G
NlaIV          29  GGATCC               GG[ACGT]^[ACGT]CC
PleI           36  GAGTC                GAGTC(4/5)
PvuII         112  CAGCTG               CAG^CTG
Tru9I          22  TTAA                 T^TAA
Tsp45I         91  GTGAC                ^GT[GC]AC
XcmI          126  CCAGAGAAAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BstYI          29  GGATCC               [GA]^GATC[CT]
               78  AGATCC              
DpnII          30  GATC                 ^GATC
               79  GATC                
MboI           30  GATC                 ^GATC
               79  GATC                
MflI           29  GGATCC               [GA]^GATC[CT]
               78  AGATCC              
NdeII          30  GATC                 ^GATC
               79  GATC                
Sau3AI         30  GATC                 ^GATC
               79  GATC                
XhoII          29  GGATCC               [GA]^GATC[CT]
               78  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

AlwI        MnlI