Restriction map for
pFN21AE6520
(Circular) (Six-base) MAPSORT of: pFN21AE6520.seq Check: 6262 from: 1 to: 4867
pFN21AE6520 4867 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2677 278
Size: 53 83 186 936 1141 2468
Fragments arranged by size:
2468 1141 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2210 1937
Size: 273 4594
Acc65I G'GTAC_C
Cuts at: 2188 2188
Size: 4867
AclI AA'CG_TT
Cuts at: 1563 2994 3367 1563
Size: 1431 373 3063
Fragments arranged by size:
3063 1431 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2930 4314 4582 1632
Size: 1298 1384 268 1917
Fragments arranged by size:
1917 1384 1298 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4670
AflIII A'CryG_T
Cuts at: 1203 3766 4743 1203
Size: 2563 977 1327
Fragments arranged by size:
2563 1327 977
AgeI A'CCGG_T
Cuts at: 3698 3698
Size: 4867
AhdI GACnn_n'nnGTC
Cuts at: 3596 3596
Size: 4867
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4867
AlwNI CAG_nnn'CTG
Cuts at: 2610 4182 2610
Size: 1572 3295
ApaLI G'TGCA_C
Cuts at: 2924 4080 2924
Size: 1156 3711
ApoI r'AATT_y
Cuts at: 1493 2176 2402 2554 4663 1493
Size: 683 226 152 2109 1697
Fragments arranged by size:
2109 1697 683 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3421 160
Size: 872 2389 1606
Fragments arranged by size:
2389 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4867
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 1870
Size: 73 249 4545
Fragments arranged by size:
4545 249 73
BamHI G'GATC_C
Cuts at: 2034 2197 2564 2034
Size: 163 367 4337
Fragments arranged by size:
4337 367 163
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2188 3643 618
Size: 325 217 627 401 1455 1842
Fragments arranged by size:
1842 1455 627 401 325 217
BanII G_rGCy'C
Cuts at: 1391 2186 1391
Size: 795 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3058 3092 1903
Size: 34 1121 34 3678
Fragments arranged by size:
3678 1121 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2759 3969 2759
Size: 1210 3657
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4867
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3478 136
Size: 107 122 71 631 605 1806 1525
Fragments arranged by size:
1806 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4862 4862
Size: 4867
BlpI GC'TnA_GC
Cuts at: 2284 2284
Size: 4867
Bme1580I G_kGCm'C
Cuts at: 1790 2928 4084 1790
Size: 1138 1156 2573
Fragments arranged by size:
2573 1156 1138
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4867
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2598 3556 442
Size: 416 53 83 399 24 1181 958 1753
Fragments arranged by size:
1753 1181 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3527 1239
Size: 2288 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2992 3857 4155 2992
Size: 865 298 3704
Fragments arranged by size:
3704 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3530 882
Size: 617 2031 2219
Fragments arranged by size:
2219 2031 617
BsaAI yAC'GTr
Cuts at: 493 1204 2658 493
Size: 711 1454 2702
Fragments arranged by size:
2702 1454 711
BsaBI GATnn'nnATC
Cuts at: 4861 4861
Size: 4867
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2674 3056 3691 275
Size: 53 83 186 936 1141 382 635 1451
Fragments arranged by size:
1451 1141 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3300 3698 3972 4119 4530 3300
Size: 398 274 147 411 3637
Fragments arranged by size:
3637 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4642
Fragments arranged by size:
4642 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4867
BseYI C'CCAG_C
Cuts at: 4070 4566 4070
Size: 496 4371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2317 3078 4106 1540
Size: 275 186 316 761 1028 2301
Fragments arranged by size:
2301 1028 761 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 2928 3013 4084 2928
Size: 85 1071 3711
Fragments arranged by size:
3711 1071 85
BsmI GAATG_Cn'
Cuts at: 2378 2471 2378
Size: 93 4774
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2186 2928 3013 4084 1391
Size: 399 396 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 85
BspEI T'CCGG_A
Cuts at: 4530 4530
Size: 4867
BspHI T'CATG_A
Cuts at: 2756 4486 2756
Size: 1730 3137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2222 844
Size: 1378 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2754 1394
Size: 429 931 3507
Fragments arranged by size:
3507 931 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3362 3536 65
Size: 1183 2114 174 1396
Fragments arranged by size:
2114 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3511 3698 1700
Size: 110 1701 187 2869
Fragments arranged by size:
2869 1701 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4867
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4867
BssSI C'ACGA_G
Cuts at: 2927 3939 2927
Size: 1012 3855
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4867
BstBI TT'CG_AA
Cuts at: 3671 3671
Size: 4867
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4867
BstYI r'GATC_y
Cuts at: 1972 2034 2083 2197 2564 2949 2966 4418 4504
Size: 62 49 114 367 385 17 1452 86
Cuts at: 4504 4516 4862 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 114 86 62
49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3376
Fragments arranged by size:
3376 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4128
Fragments arranged by size:
4128 683 56
BtsI GCAGTG_nn'
Cuts at: 2383 3177 3197 2383
Size: 794 20 4053
Fragments arranged by size:
4053 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4867
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4832
DraI TTT'AAA
Cuts at: 2171 2171
Size: 4867
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3874 809
Size: 507 2558 1802
Fragments arranged by size:
2558 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2143 2314 3203
Size: 54 1425 50 275 18 313 171 889
Cuts at: 3203 4832 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 313 275 171 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2314 1537
Size: 275 502 4090
Fragments arranged by size:
4090 502 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2797 1353
Size: 1444 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3454 3828 3974 387
Size: 1064 138 1865 374 146 1280
Fragments arranged by size:
1865 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2930 3527 4314 4582 1239
Size: 393 1298 597 787 268 1524
Fragments arranged by size:
1524 1298 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2029 2029
Size: 4867
EcoRI G'AATT_C
Cuts at: 2176 2554 2176
Size: 378 4489
FspI TGC'GCA
Cuts at: 3373 3373
Size: 4867
HaeII r_GCGC'y
Cuts at: 1678 4014 1678
Size: 2336 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2026 2058 3508 3540 3582
Size: 32 135 32 454 32 1450 32 42
Cuts at: 3582 3614 1373
Size: 32 2626
Fragments arranged by size:
2626 1450 454 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2211 2457 669
Size: 988 281 273 246 3079
Fragments arranged by size:
3079 988 281 273 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4867
HpaI GTT'AAC
Cuts at: 2457 2457
Size: 4867
KpnI G_GTAC'C
Cuts at: 2192 2192
Size: 4867
MfeI C'AATT_G
Cuts at: 2466 2466
Size: 4867
MluI A'CGCG_T
Cuts at: 4743 4743
Size: 4867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3981 4165 1305
Size: 2676 184 2007
Fragments arranged by size:
2676 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2145 10
Size: 54 1425 343 313 2732
Fragments arranged by size:
2732 1425 343 313 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2825 3184 3343 518
Size: 648 1659 359 159 2042
Fragments arranged by size:
2042 1659 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 2283 2960 4108 4353 1606
Size: 513 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 513 245 164
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3376
Fragments arranged by size:
3376 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4867
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4867
NotI GC'GGCC_GC
Cuts at: 2314 2314
Size: 4867
NspI r_CATG'y
Cuts at: 1132 1229 2225 3770 1132
Size: 97 996 1545 2229
Fragments arranged by size:
2229 1545 996 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4867
PciI A'CATG_T
Cuts at: 3766 3766
Size: 4867
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4867
PmeI GTTT'AAAC
Cuts at: 2171 2171
Size: 4867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 4449 4481 2936
Size: 32 1481 32 3322
Fragments arranged by size:
3322 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4867
PsiI TTA'TAA
Cuts at: 2437 2437
Size: 4867
PstI C_TGCA'G
Cuts at: 830 2219 830
Size: 1389 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4867
PvuII CAG'CTG
Cuts at: 2119 2119
Size: 4867
SalI G'TCGA_C
Cuts at: 1936 2209 1936
Size: 273 4594
SbfI CC_TGCA'GG
Cuts at: 2219 2219
Size: 4867
ScaI AGT'ACT
Cuts at: 3115 4827 3115
Size: 1712 3155
SfcI C'TryA_G
Cuts at: 826 1045 2215 3350 4031 4222 826
Size: 219 1170 1135 681 191 1471
Fragments arranged by size:
1471 1170 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4867
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4867
SmaI CCC'GGG
Cuts at: 1872 2194 1872
Size: 322 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2971 3872 4134 820
Size: 197 926 1028 901 262 1553
Fragments arranged by size:
1553 1028 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2658 493
Size: 2165 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4867
SphI G_CATG'C
Cuts at: 1132 2225 1132
Size: 1093 3774
SspI AAT'ATT
Cuts at: 5 52 2791 5
Size: 47 2739 2081
Fragments arranged by size:
2739 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4867
StyI C'CwwG_G
Cuts at: 513 1065 2004 2306 513
Size: 552 939 302 3074
Fragments arranged by size:
3074 939 552 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2894 2911 3064 3249 3694 325
Size: 1255 1314 17 153 185 445 1498
Fragments arranged by size:
1498 1314 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3113 4825 96
Size: 274 80 33 51 1443 1136 1712 138
Fragments arranged by size:
1712 1443 1136 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4827 4859 4827
Size: 32 4835
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4867
XbaI T'CTAG_A
Cuts at: 2203 2203
Size: 4867
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2138 2011
Size: 127 4740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4867
XmaI C'CCGG_G
Cuts at: 1870 2192 1870
Size: 322 4545
XmnI GAAnn'nnTTC
Cuts at: 2996 2996
Size: 4867
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2675 276
Size: 53 83 186 936 1141 2468
Fragments arranged by size:
2468 1141 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI