################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AciI 162 CCGC CCGC(-3/-1) AcsI 73 AAATTT [GA]^AATT[CT] AfaI 159 GTAC GT^AC AluI 35 AGCT AG^CT AlwI 145 GATCC GGATC(4/5) AocI 126 CCTGAGG CC^T[ACGT]AGG ApoI 73 AAATTT [GA]^AATT[CT] BanII 190 GAGCCC G[GA]GC[CT]^C BbvCI 174 CCTCAGC CCTCAGC(-5/-2) BpmI 31 CTCCAG CTGGAG(16/14) BsaBI 228 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 91 GAGACC GGTCTC(1/5) BsaJI 194 CCATGG C^C[ACGT][ACGT]GG BseRI 279 CTCCTC GAGGAG(10/8) BsgI 302 CTGCAC GTGCAG(16/14) BsiYI 33 CCAGCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 33 CCAGCTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 91 GAGAC GTCTC(1/5) BsmFI 238 GGGAC GGGAC(10/14) BsrFI 274 ACCGGC [GA]^CCGG[CT] BssKI 258 CCTGG ^CC[ACGT]GG BstEII 169 GGTGACC G^GT[ACGT]ACC BstNI 258 CCTGG CC^[AT]GG BstPI 169 GGTGACC G^GT[ACGT]ACC Bsu36I 126 CCTGAGG CC^T[ACGT]AGG Cac8I 110 GCTTGC GC[ACGT]^[ACGT]GC Cfr10I 274 ACCGGC [GA]^CCGG[CT] DraII 130 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 194 CCATGG C^C[GA][CT]GG EarI 287 CTCTTC CTCTTC(1/4) Eco81I 126 CCTGAGG CC^T[ACGT]AGG EcoO109I 130 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 169 GGTGACC G^GT[ACGT]ACC EcoRII 258 CCTGG ^CC[AT]GG EcoT14I 194 CCATGG C^C[AT][AT]GG HaeIII 198 GGCC GG^CC HapII 275 CCGG C^CGG HinfI 240 GACTC G^A[ACGT]TC HpaII 275 CCGG C^CGG Ksp632I 287 CTCTTC CTCTTC(1/4) MamI 228 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 77 TTAA T^TAA MslI 136 CACAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 275 CCGG C^CGG MvaI 258 CCTGG CC^[AT]GG NcoI 194 CCATGG C^CATGG PflMI 33 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 240 GACTC GAGTC(4/5) PpuMI 130 AGGACCC [GA]G^G[AT]CC[CT] RsaI 159 GTAC GT^AC ScrFI 258 CCTGG CC^[ACGT]GG StyI 194 CCATGG C^C[AT][AT]GG Tru9I 77 TTAA T^TAA Tsp509I 74 AATT ^AATT TspRI 50 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 33 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 194 CCATGGCCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaII 131 GGACC G^G[AT]CC 261 GGTCC BbvI 46 GCAGC GCAGC(8/12) 211 GCTGC BmyI 61 GTGCCC G[AGT]GC[ACT]^C 190 GAGCCC Bsp1286I 61 GTGCCC G[AGT]GC[ACT]^C 190 GAGCCC BstF5I 8 GGATG GGATG(2/0) 207 GGATG Cfr13I 131 GGACC G^G[ACGT]CC 261 GGTCC DdeI 127 CTGAG C^T[ACGT]AG 175 CTCAG DpnII 145 GATC ^GATC 228 GATC Fnu4HI 46 GCAGC GC^[ACGT]GC 211 GCTGC FokI 8 GGATG GGATG(9/13) 207 GGATG HphI 169 GGTGA GGTGA(8/7) 223 GGTGA ItaI 46 GCAGC GC^[ACGT]GC 211 GCTGC MaeIII 170 GTGAC ^GT[ACGT]AC 181 GTGAC MboI 145 GATC ^GATC 228 GATC MboII 69 GAAGA GAAGA(8/7) 288 TCTTC NdeII 145 GATC ^GATC 228 GATC NlaIV 131 GGACCC GG[ACGT]^[ACGT]CC 277 GGCTCC Sau3AI 145 GATC ^GATC 228 GATC Sau96I 131 GGACC G^G[ACGT]CC 261 GGTCC SfaNI 208 GATGC GCATC(5/9) 233 GCATC TseI 46 GCAGC G^C[AT]GC 211 GCTGC Tsp45I 170 GTGAC ^GT[GC]AC 181 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MnlI NlaIII