(Linear) (Six-base) MAPSORT of: KIEE5142.seq  Check: 4484  from: 1  to: 312



KIEE5142.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     312 
   Size:         88     224
 
ApoI r'AATT_y

Cuts at:      0      73     312 
   Size:         73     239
 
BanII G_rGCy'C

Cuts at:      0     194     312 
   Size:        194     118
 
BbvCI CC'TCA_GC

Cuts at:      0     175     312 
   Size:        175     137
 
Bme1580I G_kGCm'C

Cuts at:      0      65     312 
   Size:         65     247
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     312 
   Size:         16     296
 
Bpu10I CC'TnA_GC

Cuts at:      0     175     312 
   Size:        175     137
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      85     312 
   Size:         85     227
 
BsaBI GATnn'nnATC

Cuts at:      0     232     312 
   Size:        232      80
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     262     292     312 
   Size:        262      30      20

  Fragments arranged by size: 

                 262      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     270     312 
   Size:        270      42
 
BseYI C'CCAG_C

Cuts at:      0     114     312 
   Size:        114     198
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     312 
   Size:        287      25
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     194     312 
   Size:         65     129     118

  Fragments arranged by size: 

                 129     118      65
 
BsrFI r'CCGG_y

Cuts at:      0     274     312 
   Size:        274      38
 
BstEII G'GTnAC_C

Cuts at:      0     169     312 
   Size:        169     143
 
Bsu36I CC'TnA_GG

Cuts at:      0     127     312 
   Size:        127     185
 
BtgI C'CryG_G

Cuts at:      0     194     312 
   Size:        194     118
 
BtsI GCAGTG_nn'

Cuts at:      0      56     312 
   Size:         56     256
 
EarI CTCTTCn'nnn_

Cuts at:      0     293     312 
   Size:        293      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16      88     312 
   Size:         16      72     224

  Fragments arranged by size: 

                 224      72      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     131     312 
   Size:        131     181
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     180     312 
   Size:        180     132
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     262     294     312 
   Size:        262      32      18

  Fragments arranged by size: 

                 262      32      18
 
MslI CAynn'nnrTG

Cuts at:      0     140     312 
   Size:        140     172
 
NcoI C'CATG_G

Cuts at:      0     194     312 
   Size:        194     118
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      39     312 
   Size:         39     273
 
PpuMI rG'GwC_Cy

Cuts at:      0     131     312 
   Size:        131     181
 
StyI C'CwwG_G

Cuts at:      0     194     312 
   Size:        194     118
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     201     312 
   Size:        201     111
 

 Enzymes that do cut:

     AcuI     ApoI    BanII    BbvCI Bme1580I     BpmI   Bpu10I     BsaI
    BsaBI    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BsrFI   BstEII
   Bsu36I     BtgI     BtsI     EarI  Eco57MI EcoO109I     FalI    Hin4I
     MslI     NcoI    PflMI    PpuMI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaHI
    BsaWI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI