Restriction map for
pFN21AE6522
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DrdI DsaI
Eam1105I EarI Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FokI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 247 GTAC GT^AC
AflIII 126 ACGTGT A^C[GA][CT]GT
AvaII 18 GGACC G^G[AT]CC
BfaI 244 CTAG C^TAG
BglII 153 AGATCT A^GATCT
BsaI 230 GGTCTC GGTCTC(1/5)
BseRI 49 GAGGAG GAGGAG(10/8)
BsgI 70 GTGCAG GTGCAG(16/14)
BsrBI 9 GAGCGG CCGCTC(-3/-3)
BsrFI 37 GCCGGC [GA]^CCGG[CT]
BstYI 153 AGATCT [GA]^GATC[CT]
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
DraIII 175 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 111 CCTTACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 84 GCGGG CCCGC(4/6)
FseI 36 GGCCGGCC GGCCGG^CC
HapII 38 CCGG C^CGG
HpaII 38 CCGG C^CGG
HphI 174 TCACC GGTGA(8/7)
MaeI 244 CTAG C^TAG
MaeII 126 ACGT A^CGT
MaeIII 160 GTGAC ^GT[ACGT]AC
MboII 184 GAAGA GAAGA(8/7)
MflI 153 AGATCT [GA]^GATC[CT]
MseI 190 TTAA T^TAA
MspI 38 CCGG C^CGG
NaeI 37 GCCGGC GCC^GGC
NgoMIV 37 GCCGGC G^CCGGC
NlaIII 252 CATG CATG^
NlaIV 40 GGCCCC GG[ACGT]^[ACGT]CC
PstI 149 CTGCAG CTGCA^G
RsaI 247 GTAC GT^AC
SfcI 149 CTGCAG C^T[GA][CT]AG
Tru9I 190 TTAA T^TAA
Tsp45I 160 GTGAC ^GT[GC]AC
Tsp509I 192 AATT ^AATT
XhoII 153 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 74 AGCT AG^CT
147 AGCT
BsaJI 43 CCCTGG C^C[ACGT][ACGT]GG
44 CCTGGG
BsiEI 35 CGGCCG CG[GA][CT]^CG
65 CGGCCG
BsmAI 130 GTCTC GTCTC(1/5)
231 GTCTC
CfoI 28 GCGC GCG^C
79 GCGC
Cfr13I 18 GGACC G^G[ACGT]CC
40 GGCCC
DdeI 116 CTGAG C^T[ACGT]AG
200 CTGAG
EaeI 35 CGGCCG [CT]^GGCC[GA]
65 CGGCCG
EagI 35 CGGCCG C^GGCCG
65 CGGCCG
EclXI 35 CGGCCG C^GGCCG
65 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
65 CGGCCG
HaeII 27 GGCGCT [GA]GCGC^[CT]
78 GGCGCT
HhaI 28 GCGC GCG^C
79 GCGC
HinP1I 28 GCGC G^CGC
79 GCGC
MspA1I 73 CAGCTG C[AC]G^C[GT]G
146 CAGCTG
PvuII 73 CAGCTG CAG^CTG
146 CAGCTG
Sau96I 18 GGACC G^G[ACGT]CC
40 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI BbvI BsiYI BslI BssKI
BstNI Cac8I DpnII EcoRII Fnu4HI
HaeIII ItaI MboI MnlI MvaI
MwoI NdeII Sau3AI ScrFI TseI