Restriction map for
pFN21AE6522
(Circular) (Six-base) MAPSORT of: pFN21AE6522.seq Check: 5040 from: 1 to: 4960
pFN21AE6522 4960 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2770 278
Size: 53 83 186 936 1234 2468
Fragments arranged by size:
2468 1234 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2303 1937
Size: 366 4594
Acc65I G'GTAC_C
Cuts at: 2281 2281
Size: 4960
AclI AA'CG_TT
Cuts at: 1563 3087 3460 1563
Size: 1524 373 3063
Fragments arranged by size:
3063 1524 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3023 4407 4675 1632
Size: 1391 1384 268 1917
Fragments arranged by size:
1917 1391 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4763
AflIII A'CryG_T
Cuts at: 1203 2131 3859 4836 1203
Size: 928 1728 977 1327
Fragments arranged by size:
1728 1327 977 928
AgeI A'CCGG_T
Cuts at: 3791 3791
Size: 4960
AhdI GACnn_n'nnGTC
Cuts at: 3689 3689
Size: 4960
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4960
AlwNI CAG_nnn'CTG
Cuts at: 2703 4275 2703
Size: 1572 3388
ApaLI G'TGCA_C
Cuts at: 3017 4173 3017
Size: 1156 3804
ApoI r'AATT_y
Cuts at: 1493 2269 2495 2647 4756 1493
Size: 776 226 152 2109 1697
Fragments arranged by size:
2109 1697 776 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3514 160
Size: 872 2482 1606
Fragments arranged by size:
2482 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4960
AvaI C'yCGr_G
Cuts at: 1870 1943 2285 1870
Size: 73 342 4545
Fragments arranged by size:
4545 342 73
BamHI G'GATC_C
Cuts at: 2290 2657 2290
Size: 367 4593
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2281 3736 618
Size: 325 217 627 494 1455 1842
Fragments arranged by size:
1842 1455 627 494 325 217
BanII G_rGCy'C
Cuts at: 1391 2279 1391
Size: 888 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3151 3185 1903
Size: 34 1214 34 3678
Fragments arranged by size:
3678 1214 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2852 4062 2852
Size: 1210 3750
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4960
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3571 136
Size: 107 122 71 631 605 1899 1525
Fragments arranged by size:
1899 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2158 4955 2158
Size: 2797 2163
BlpI GC'TnA_GC
Cuts at: 2377 2377
Size: 4960
Bme1580I G_kGCm'C
Cuts at: 1790 3021 4177 1790
Size: 1231 1156 2573
Fragments arranged by size:
2573 1231 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4960
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2691 3649 442
Size: 416 53 83 399 24 1274 958 1753
Fragments arranged by size:
1753 1274 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3620 1239
Size: 2381 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3085 3950 4248 3085
Size: 865 298 3797
Fragments arranged by size:
3797 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2241 3623 882
Size: 617 742 1382 2219
Fragments arranged by size:
2219 1382 742 617
BsaAI yAC'GTr
Cuts at: 493 1204 2751 493
Size: 711 1547 2702
Fragments arranged by size:
2702 1547 711
BsaBI GATnn'nnATC
Cuts at: 4954 4954
Size: 4960
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2767 3149 3784 275
Size: 53 83 186 936 1234 382 635 1451
Fragments arranged by size:
1451 1234 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3393 3791 4065 4212 4623 3393
Size: 398 274 147 411 3730
Fragments arranged by size:
3730 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4735
Fragments arranged by size:
4735 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 1186
Size: 883 4077
BseYI C'CCAG_C
Cuts at: 4163 4659 4163
Size: 496 4464
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 1163
Size: 573 360 4027
Fragments arranged by size:
4027 573 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2043 2073 2410 3171 4199 1540
Size: 275 186 42 30 337 761 1028 2301
Fragments arranged by size:
2301 1028 761 337 275 186 42 30
BsiHKAI G_wGCw'C
Cuts at: 3021 3106 4177 3021
Size: 85 1071 3804
Fragments arranged by size:
3804 1071 85
BsmI GAATG_Cn'
Cuts at: 2471 2564 2471
Size: 93 4867
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2279 3021 3106 4177 1391
Size: 399 489 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 489 399 85
BspEI T'CCGG_A
Cuts at: 4623 4623
Size: 4960
BspHI T'CATG_A
Cuts at: 2849 4579 2849
Size: 1730 3230
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2315 844
Size: 1471 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 2847 1394
Size: 429 193 831 3507
Fragments arranged by size:
3507 831 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 3455 3629 65
Size: 1183 2207 174 1396
Fragments arranged by size:
2207 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 3604 3791 1700
Size: 110 232 1562 187 2869
Fragments arranged by size:
2869 1562 232 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4960
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4960
BssSI C'ACGA_G
Cuts at: 3020 4032 3020
Size: 1012 3948
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4960
BstBI TT'CG_AA
Cuts at: 3764 3764
Size: 4960
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4960
BstYI r'GATC_y
Cuts at: 1972 2158 2290 2657 3042 3059 4511 4597 4609
Size: 186 132 367 385 17 1452 86 12
Cuts at: 4609 4955 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 186 132 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4408
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4221
Fragments arranged by size:
4221 683 56
BtsI GCAGTG_nn'
Cuts at: 2476 3270 3290 2476
Size: 794 20 4146
Fragments arranged by size:
4146 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4960
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4925
DraI TTT'AAA
Cuts at: 2264 2264
Size: 4960
DraIII CAC_nnn'GTG
Cuts at: 2185 2185
Size: 4960
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3967 809
Size: 507 2651 1802
Fragments arranged by size:
2651 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2070 2407
Size: 54 1425 50 275 18 210 30 337
Cuts at: 2407 3296 4925 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 337 275 210 54 50
43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2040 2070 2407 1537
Size: 275 228 30 337 4090
Fragments arranged by size:
4090 337 275 228 30
EarI CTCTTCn'nnn_
Cuts at: 1353 2890 1353
Size: 1537 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3547 3921 4067 387
Size: 1064 138 1958 374 146 1280
Fragments arranged by size:
1958 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3023 3620 4407 4675 1239
Size: 393 1391 597 787 268 1524
Fragments arranged by size:
1524 1391 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 4960
EcoRI G'AATT_C
Cuts at: 2269 2647 2269
Size: 378 4582
FseI GG_CCGG'CC
Cuts at: 2046 2046
Size: 4960
FspI TGC'GCA
Cuts at: 3466 3466
Size: 4960
HaeII r_GCGC'y
Cuts at: 1678 2036 2087 4107 1678
Size: 358 51 2020 2531
Fragments arranged by size:
2531 2020 358 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2088 2120 3601 3633 3675
Size: 32 135 32 516 32 1481 32 42
Cuts at: 3675 3707 1373
Size: 32 2626
Fragments arranged by size:
2626 1481 516 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2304 2550 669
Size: 988 281 366 246 3079
Fragments arranged by size:
3079 988 366 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4960
HpaI GTT'AAC
Cuts at: 2550 2550
Size: 4960
KpnI G_GTAC'C
Cuts at: 2285 2285
Size: 4960
MfeI C'AATT_G
Cuts at: 2559 2559
Size: 4960
MluI A'CGCG_T
Cuts at: 4836 4836
Size: 4960
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4074 4258 1305
Size: 2769 184 2007
Fragments arranged by size:
2769 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3138
Fragments arranged by size:
3138 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2918 3277 3436 518
Size: 648 1752 359 159 2042
Fragments arranged by size:
2042 1752 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2080 2153 2376 3053 4201 4446 1606
Size: 474 73 223 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 474 245 223 73
NaeI GCC'GGC
Cuts at: 2044 2044
Size: 4960
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4408
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4960
NgoMIV G'CCGG_C
Cuts at: 2042 2042
Size: 4960
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4960
NotI GC'GGCC_GC
Cuts at: 2407 2407
Size: 4960
NspI r_CATG'y
Cuts at: 1132 1229 2318 3863 1132
Size: 97 1089 1545 2229
Fragments arranged by size:
2229 1545 1089 97
PasI CC'CwG_GG
Cuts at: 1348 2049 1348
Size: 701 4259
PciI A'CATG_T
Cuts at: 3859 3859
Size: 4960
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4960
PmeI GTTT'AAAC
Cuts at: 2264 2264
Size: 4960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3029 3061 4542 4574 3029
Size: 32 1481 32 3415
Fragments arranged by size:
3415 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4960
PsiI TTA'TAA
Cuts at: 2530 2530
Size: 4960
PstI C_TGCA'G
Cuts at: 830 2158 2312 830
Size: 1328 154 3478
Fragments arranged by size:
3478 1328 154
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4960
PvuII CAG'CTG
Cuts at: 2080 2153 2080
Size: 73 4887
SalI G'TCGA_C
Cuts at: 1936 2302 1936
Size: 366 4594
SbfI CC_TGCA'GG
Cuts at: 2312 2312
Size: 4960
ScaI AGT'ACT
Cuts at: 3208 4920 3208
Size: 1712 3248
SfcI C'TryA_G
Cuts at: 826 1045 2154 2308 3443 4124 4315 826
Size: 219 1109 154 1135 681 191 1471
Fragments arranged by size:
1471 1135 1109 681 219 191 154
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4960
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4960
SmaI CCC'GGG
Cuts at: 1872 2287 1872
Size: 415 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3064 3965 4227 820
Size: 197 926 1121 901 262 1553
Fragments arranged by size:
1553 1121 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2751 493
Size: 2258 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4960
SphI G_CATG'C
Cuts at: 1132 2318 1132
Size: 1186 3774
SspI AAT'ATT
Cuts at: 5 52 2884 5
Size: 47 2832 2081
Fragments arranged by size:
2832 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4960
StyI C'CwwG_G
Cuts at: 513 1065 2399 513
Size: 552 1334 3074
Fragments arranged by size:
3074 1334 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2987 3004 3157 3342 3787 325
Size: 1255 1407 17 153 185 445 1498
Fragments arranged by size:
1498 1407 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2251 3206 4918
Size: 274 80 33 51 1443 274 955 1712
Cuts at: 4918 96
Size: 138
Fragments arranged by size:
1712 1443 955 274 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4920 4952 4920
Size: 32 4928
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4960
XbaI T'CTAG_A
Cuts at: 2296 2296
Size: 4960
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4960
XmaI C'CCGG_G
Cuts at: 1870 2285 1870
Size: 415 4545
XmnI GAAnn'nnTTC
Cuts at: 3089 3089
Size: 4960
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2768 276
Size: 53 83 186 936 1234 2468
Fragments arranged by size:
2468 1234 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoO109I EcoRV FalI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI