(Linear) (Six-base) MAPSORT of: KIEE6522.seq  Check: 6495  from: 1  to: 255



KIEE6522.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AflIII A'CryG_T

Cuts at:      0     126     255 
   Size:        126     129
 
BglII A'GATC_T

Cuts at:      0     153     255 
   Size:        153     102
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     236     255 
   Size:        236      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     255 
   Size:         64     191
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     255 
   Size:         91     164
 
BsiEI CG_ry'CG

Cuts at:      0      38      68     255 
   Size:         38      30     187

  Fragments arranged by size: 

                 187      38      30
 
BsrBI CCG'CTC

Cuts at:      0      11     255 
   Size:         11     244
 
BsrFI r'CCGG_y

Cuts at:      0      37     255 
   Size:         37     218
 
BstYI r'GATC_y

Cuts at:      0     153     255 
   Size:        153     102
 
DraIII CAC_nnn'GTG

Cuts at:      0     180     255 
   Size:        180      75
 
EaeI y'GGCC_r

Cuts at:      0      35      65     255 
   Size:         35      30     190

  Fragments arranged by size: 

                 190      35      30
 
EagI C'GGCC_G

Cuts at:      0      35      65     255 
   Size:         35      30     190

  Fragments arranged by size: 

                 190      35      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115     255 
   Size:        115     140
 
FseI GG_CCGG'CC

Cuts at:      0      41     255 
   Size:         41     214
 
HaeII r_GCGC'y

Cuts at:      0      31      82     255 
   Size:         31      51     173

  Fragments arranged by size: 

                 173      51      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      83     115     255 
   Size:         83      32     140

  Fragments arranged by size: 

                 140      83      32
 
MspA1I CmG'CkG

Cuts at:      0      75     148     255 
   Size:         75      73     107

  Fragments arranged by size: 

                 107      75      73
 
NaeI GCC'GGC

Cuts at:      0      39     255 
   Size:         39     216
 
NgoMIV G'CCGG_C

Cuts at:      0      37     255 
   Size:         37     218
 
PasI CC'CwG_GG

Cuts at:      0      44     255 
   Size:         44     211
 
PstI C_TGCA'G

Cuts at:      0     153     255 
   Size:        153     102
 
PvuII CAG'CTG

Cuts at:      0      75     148     255 
   Size:         75      73     107

  Fragments arranged by size: 

                 107      75      73
 
SfcI C'TryA_G

Cuts at:      0     149     255 
   Size:        149     106
 
TatI w'GTAC_w

Cuts at:      0     246     255 
   Size:        246       9
 

 Enzymes that do cut:

   AflIII    BglII     BsaI    BseRI     BsgI    BsiEI    BsrBI    BsrFI
    BstYI   DraIII     EaeI     EagI    EcoNI     FseI    HaeII    Hin4I
   MspA1I     NaeI   NgoMIV     PasI     PstI    PvuII     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI