################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI AlwNI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbvCI BcgI BciVI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 1371 GTCTAC GT^[AC][GT]AC AflII 415 CTTAAG C^TTAAG Alw44I 1637 GTGCAC G^TGCAC AlwI 255 GATCC GGATC(4/5) AocI 1042 CCTCAGG CC^T[ACGT]AGG ApaLI 1637 GTGCAC G^TGCAC AseI 946 ATTAAT AT^TAAT AsnI 946 ATTAAT AT^TAAT AvaII 1757 GGACC G^G[AT]CC BalI 896 TGGCCA TGG^CCA BanII 397 GAGCTC G[GA]GC[CT]^C BbrPI 671 CACGTG CAC^GTG BbsI 1449 GTCTTC GAAGAC(2/6) BclI 507 TGATCA T^GATCA BcnI 974 CCCGG CC^[GC]GG BfrI 415 CTTAAG C^TTAAG BglII 328 AGATCT A^GATCT BpmI 1289 CTGGAG CTGGAG(16/14) BpuAI 1449 GTCTTC GAAGAC(2/6) BsaAI 671 CACGTG [CT]AC^GT[GA] BsaI 1727 GAGACC GGTCTC(1/5) BspMI 886 ACCTGC ACCTGC(4/8) BstXI 798 CCATTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 328 AGATCT [GA]^GATC[CT] Bsu36I 1042 CCTCAGG CC^T[ACGT]AGG Cac8I 821 GCTGGC GC[ACGT]^[ACGT]GC CfoI 277 GCGC GCG^C DraII 1718 GGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 896 TGGCCA [CT]^GGCC[GA] Eco81I 1042 CCTCAGG CC^T[ACGT]AGG EcoO109I 1718 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRV 1034 GATATC GAT^ATC FauI 25 GCGGG CCCGC(4/6) FbaI 507 TGATCA T^GATCA HapII 975 CCGG C^CGG HhaI 277 GCGC GCG^C HinP1I 277 GCGC G^CGC HincII 1258 GTCAAC GT[CT]^[GA]AC HindII 1258 GTCAAC GT[CT]^[GA]AC HindIII 531 AAGCTT A^AGCTT HpaII 975 CCGG C^CGG HphI 601 TCACC GGTGA(8/7) MaeII 672 ACGT A^CGT MflI 328 AGATCT [GA]^GATC[CT] MluNI 896 TGGCCA TGG^CCA MscI 896 TGGCCA TGG^CCA MspI 975 CCGG C^CGG MwoI 1681 GCCATGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 974 CCCGG CC^[GC]GG PmaCI 671 CACGTG CAC^GTG PmlI 671 CACGTG CAC^GTG PshBI 946 ATTAAT AT^TAAT SacI 397 GAGCTC GAGCT^C SapI 739 GAAGAGC GCTCTTC(1/4) XbaI 1339 TCTAGA T^CTAGA XhoII 328 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 82 CGCG CG^CG 278 CGCG AflIII 670 ACACGT A^C[GA][CT]GT 672 ACGTGT Asp700I 161 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1072 GAATATTTTC BbvI 19 GCTGC GCAGC(8/12) 1687 GCTGC BsmAI 1727 GAGAC GTCTC(1/5) 487 GTCTC BstUI 82 CGCG CG^CG 278 CGCG Cfr13I 1719 GGGCC G^G[ACGT]CC 1757 GGACC DraI 333 TTTAAA TTT^AAA 1194 TTTAAA DsaI 272 CCATGG C^C[GA][CT]GG 1682 CCATGG MvnI 82 CGCG CG^CG 278 CGCG NcoI 272 CCATGG C^CATGG 1682 CCATGG NlaIV 1718 GGGGCC GG[ACGT]^[ACGT]CC 1757 GGACCC PleI 87 GAGTC GAGTC(4/5) 1473 GAGTC PstI 422 CTGCAG CTGCA^G 1512 CTGCAG Sau96I 1719 GGGCC G^G[ACGT]CC 1757 GGACC ScaI 1383 AGTACT AGT^ACT 1594 AGTACT SspI 832 AATATT AAT^ATT 1073 AATATT TaqI 1471 TCGA T^CGA 1652 TCGA TseI 19 GCTGC G^C[AT]GC 1687 GCTGC Tsp45I 1664 GTGAC ^GT[GC]AC 1745 GTGAC TthHB8I 1471 TCGA T^CGA 1652 TCGA XmnI 161 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1072 GAATATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BfaI BmyI BsaJI BseRI BsiHKAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I Tsp509I TspRI