Restriction map for
pFN21AB7870
(Circular) (Six-base) MAPSORT of: pFN21AB7870.seq Check: 4561 from: 1 to: 6469
pFN21AB7870 6469 bp
With 182 enzymes: SgfI *
March 1, 2012 18:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3377 3812 1937
Size: 1440 435 4594
Fragments arranged by size:
4594 1440 435
Acc65I G'GTAC_C
Cuts at: 3790 3790
Size: 6469
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 3730 4532 5916 6184 1632
Size: 743 1355 802 1384 268 1917
Fragments arranged by size:
1917 1384 1355 802 743 268
AflII C'TTAA_G
Cuts at: 820 1017 2420 820
Size: 197 1403 4869
Fragments arranged by size:
4869 1403 197
AflIII A'CryG_T
Cuts at: 1203 2675 2677 5368 6345 1203
Size: 1472 2 2691 977 1327
Fragments arranged by size:
2691 1472 1327 977 2
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6469
AlwNI CAG_nnn'CTG
Cuts at: 4212 5784 4212
Size: 1572 4897
ApaLI G'TGCA_C
Cuts at: 3642 4526 5682 3642
Size: 884 1156 4429
Fragments arranged by size:
4429 1156 884
ApoI r'AATT_y
Cuts at: 1493 2353 2469 2789 2969 2999 3167 3429 3778
Size: 860 116 320 180 30 168 262 349
Cuts at: 3778 4004 4156 6265 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 860 349 320 262 226 180
168 152 116 30
AseI AT'TA_AT
Cuts at: 160 1032 2952 5023 160
Size: 872 1920 2071 1606
Fragments arranged by size:
2071 1920 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 3794 1870
Size: 73 1851 4545
Fragments arranged by size:
4545 1851 73
BamHI G'GATC_C
Cuts at: 3799 4166 3799
Size: 367 6102
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3790 5245 618
Size: 325 217 627 2003 1455 1842
Fragments arranged by size:
2003 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2406 3788 1391
Size: 1015 1382 4072
Fragments arranged by size:
4072 1382 1015
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3447 928
Size: 971 1548 3950
Fragments arranged by size:
3950 1548 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4660 4694 1903
Size: 34 2723 34 3678
Fragments arranged by size:
3678 2723 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 2512 1548
Size: 964 5505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5080 136
Size: 107 122 71 631 605 337 3071 1525
Fragments arranged by size:
3071 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2333 6464 2333
Size: 4131 2338
BlpI GC'TnA_GC
Cuts at: 3886 3886
Size: 6469
Bme1580I G_kGCm'C
Cuts at: 1790 3646 4530 5686 1790
Size: 1856 884 1156 2573
Fragments arranged by size:
2573 1856 1156 884
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6469
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4200 5158 442
Size: 416 53 83 399 24 2783 958 1753
Fragments arranged by size:
2783 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3315 5129 1239
Size: 2076 1814 2579
Fragments arranged by size:
2579 2076 1814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2350 2677 2839 4594 5459 5757 2350
Size: 327 162 1755 865 298 3062
Fragments arranged by size:
3062 1755 865 327 298 162
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3726 5132 882
Size: 617 2227 1406 2219
Fragments arranged by size:
2227 2219 1406 617
BsaAI yAC'GTr
Cuts at: 493 1204 2678 4260 493
Size: 711 1474 1582 2702
Fragments arranged by size:
2702 1582 1474 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4276 4658 5293 275
Size: 53 83 186 936 2743 382 635 1451
Fragments arranged by size:
2743 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4902 5300 5574 5721 6132 4902
Size: 398 274 147 411 5239
Fragments arranged by size:
5239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2025 2045 2055 2075 1178
Size: 30 165 30 622 20 10 20 5572
Fragments arranged by size:
5572 622 165 30 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2066 2230 3620 1186
Size: 847 33 164 1390 4035
Fragments arranged by size:
4035 1390 847 164 33
BseYI C'CCAG_C
Cuts at: 5672 6168 5672
Size: 496 5973
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5896
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3919 4680 5708 1540
Size: 275 186 1918 761 1028 2301
Fragments arranged by size:
2301 1918 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2406 2784 3646 4530 4615 5686 2406
Size: 378 862 884 85 1071 3189
Fragments arranged by size:
3189 1071 884 862 378 85
BsmI GAATG_Cn'
Cuts at: 3980 4073 3980
Size: 93 6376
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2406 2784 3646 3788 4530 4615 5686
Size: 399 616 378 862 142 742 85 1071
Cuts at: 5686 1391
Size: 2174
Fragments arranged by size:
2174 1071 862 742 616 399 378 142
85
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2900 3824 844
Size: 2056 924 3489
Fragments arranged by size:
3489 2056 924
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 1394
Size: 429 2533 3507
Fragments arranged by size:
3507 2533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2064 2262 2803 4964 5138 65
Size: 1183 816 198 541 2161 174 1396
Fragments arranged by size:
2161 1396 1183 816 541 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5113 5300 1700
Size: 110 3303 187 2869
Fragments arranged by size:
3303 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 4529 5541 4529
Size: 1012 5457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6469
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6469
BstXI CCAn_nnnn'nTGG
Cuts at: 2810 2810
Size: 6469
BstYI r'GATC_y
Cuts at: 1972 2333 3799 4166 4551 4568 6020 6106 6118
Size: 361 1466 367 385 17 1452 86 12
Cuts at: 6118 6464 1972
Size: 346 1977
Fragments arranged by size:
1977 1466 1452 385 367 361 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3048 3048
Size: 6469
BtgI C'CryG_G
Cuts at: 513 1065 2004 2277 3687 513
Size: 552 939 273 1410 3295
Fragments arranged by size:
3295 1410 939 552 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5730
Fragments arranged by size:
5730 683 56
BtsI GCAGTG_nn'
Cuts at: 2994 3985 4779 4799 2994
Size: 991 794 20 4664
Fragments arranged by size:
4664 991 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6434
DraI TTT'AAA
Cuts at: 2340 3201 3773 2340
Size: 861 572 5036
Fragments arranged by size:
5036 861 572
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5476 809
Size: 507 4160 1802
Fragments arranged by size:
4160 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2901 3916 4805
Size: 54 1425 50 275 18 1071 1015 889
Cuts at: 4805 6434 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1071 1015 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2010 2480 2739 4399 1353
Size: 657 470 259 1660 3423
Fragments arranged by size:
3423 1660 657 470 259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 5056 5430 5576 387
Size: 1064 138 438 3029 374 146 1280
Fragments arranged by size:
3029 1280 1064 438 374 146 138
EcoICRI GAG'CTC
Cuts at: 2404 2404
Size: 6469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 3315 3730 4532 5129 5916 6184
Size: 393 743 940 415 802 597 787 268
Cuts at: 6184 1239
Size: 1524
Fragments arranged by size:
1524 940 802 787 743 597 415 393
268
EcoO109I rG'GnC_Cy
Cuts at: 3724 3724
Size: 6469
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
EcoRV GAT'ATC
Cuts at: 3041 3041
Size: 6469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2718 2718
Size: 6469
FspI TGC'GCA
Cuts at: 4975 4975
Size: 6469
HaeII r_GCGC'y
Cuts at: 1678 5616 1678
Size: 3938 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2400 2432 5110 5142 5184
Size: 32 135 32 828 32 2678 32 42
Cuts at: 5184 5216 1373
Size: 32 2626
Fragments arranged by size:
2678 2626 828 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3265 3813 4059 669
Size: 988 281 1327 548 246 3079
Fragments arranged by size:
3079 1327 988 548 281 246
HindIII A'AGCT_T
Cuts at: 748 2536 748
Size: 1788 4681
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KpnI G_GTAC'C
Cuts at: 3794 3794
Size: 6469
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2431 2693 2824 3506 3630 5583 5767 1305
Size: 1126 262 131 682 124 1953 184 2007
Fragments arranged by size:
2007 1953 1126 682 262 184 131 124
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2903 10
Size: 54 1425 343 1071 3576
Fragments arranged by size:
3576 1425 1071 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3090 3618 3686 4427 4786 4945 518
Size: 648 1924 528 68 741 359 159 2042
Fragments arranged by size:
2042 1924 741 648 528 359 159 68
MspA1I CmG'CkG
Cuts at: 1606 3885 4562 5710 5955 1606
Size: 2279 677 1148 245 2120
Fragments arranged by size:
2279 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2277 3687 513
Size: 552 939 273 1410 3295
Fragments arranged by size:
3295 1410 939 552 273
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 3827 5372 1132
Size: 97 2598 1545 2229
Fragments arranged by size:
2598 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3737 1348
Size: 2389 4080
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6469
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PmlI CAC'GTG
Cuts at: 2678 2678
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 2975 4039 2975
Size: 1064 5405
PstI C_TGCA'G
Cuts at: 830 2431 3521 3821 830
Size: 1601 1090 300 3478
Fragments arranged by size:
3478 1601 1090 300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
SacI G_AGCT'C
Cuts at: 2406 2406
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SapI GCTCTTCn'nnn_
Cuts at: 2739 2739
Size: 6469
SbfI CC_TGCA'GG
Cuts at: 3821 3821
Size: 6469
ScaI AGT'ACT
Cuts at: 3390 3601 4717 6429 3390
Size: 211 1116 1712 3430
Fragments arranged by size:
3430 1712 1116 211
SfcI C'TryA_G
Cuts at: 826 1045 2427 3131 3517 3817 4952 5633 5824
Size: 219 1382 704 386 300 1135 681 191
Cuts at: 5824 826
Size: 1471
Fragments arranged by size:
1471 1382 1135 704 681 386 300 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2329 2420 2692 2818 4573 5474
Size: 197 926 386 91 272 126 1755 901
Cuts at: 5474 5736 820
Size: 262 1553
Fragments arranged by size:
1755 1553 926 901 386 272 262 197
126 91
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 3827 1132
Size: 2695 3774
SspI AAT'ATT
Cuts at: 5 52 2839 3080 4393 5
Size: 47 2787 241 1313 2081
Fragments arranged by size:
2787 2081 1313 241 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6469
StyI C'CwwG_G
Cuts at: 513 1065 2004 2277 3230 3687 3908 513
Size: 552 939 273 953 457 221 3074
Fragments arranged by size:
3074 953 939 552 457 273 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4496 4513 4666 4851 5296 325
Size: 1255 2916 17 153 185 445 1498
Fragments arranged by size:
2916 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3388 3599 4715
Size: 274 80 33 51 1443 1411 211 1116
Cuts at: 4715 6427 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1411 1116 274 211 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2025 2045 2057 2077 6429 6461 2025
Size: 20 12 20 4352 32 2033
Fragments arranged by size:
4352 2033 32 20 20 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 3344 3805 3344
Size: 461 6008
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6469
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 2170 3081 4598 2170
Size: 911 1517 4041
Fragments arranged by size:
4041 1517 911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI