Restriction map for
pFN21AE2405
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
Sau96I SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI StyI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AciI 448 CCGC CCGC(-3/-1)
AlwI 799 GATCC GGATC(4/5)
AlwNI 145 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 158 CCTCAGG CC^T[ACGT]AGG
AspHI 420 GTGCTC G[AT]GC[AT]^C
BalI 1044 TGGCCA TGG^CCA
BbvCI 933 GCTGAGG CCTCAGC(-5/-2)
BciVI 186 GTATCC GTATCC(6/5)
BmyI 420 GTGCTC G[AGT]GC[ACT]^C
BsaJI 80 CCGTGG C^C[ACGT][ACGT]GG
BseRI 135 CTCCTC GAGGAG(10/8)
BsiHKAI 420 GTGCTC G[AT]GC[AT]^C
Bsp1286I 420 GTGCTC G[AGT]GC[ACT]^C
BspHI 926 TCATGA T^CATGA
BstYI 798 AGATCC [GA]^GATC[CT]
Bsu36I 158 CCTCAGG CC^T[ACGT]AGG
DsaI 80 CCGTGG C^C[GA][CT]GG
Eco81I 158 CCTCAGG CC^T[ACGT]AGG
HgaI 96 GACGC GACGC(5/10)
HindIII 371 AAGCTT A^AGCTT
MaeII 260 ACGT A^CGT
MflI 798 AGATCC [GA]^GATC[CT]
MluNI 1044 TGGCCA TGG^CCA
MscI 1044 TGGCCA TGG^CCA
NlaIV 32 GGTTCC GG[ACGT]^[ACGT]CC
PleI 307 GACTC GAGTC(4/5)
RcaI 926 TCATGA T^CATGA
SapI 918 GCTCTTC GCTCTTC(1/4)
ScaI 727 AGTACT AGT^ACT
SpeI 621 ACTAGT A^CTAGT
TfiI 748 GATTC G^A[AT]TC
Tsp45I 115 GTCAC ^GT[GC]AC
XhoII 798 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AflIII 405 ACATGT A^C[GA][CT]GT
945 ACATGT
BbvI 717 GCAGC GCAGC(8/12)
867 GCTGC
BpmI 142 CTCCAG CTGGAG(16/14)
682 CTGGAG
BsaWI 487 TCCGGT [AT]^CCGG[AT]
635 TCCGGT
BsiYI 140 CCCTCCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
155 CCCCCTCAGGG
BslI 140 CCCTCCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
155 CCCCCTCAGGG
BsmAI 664 GAGAC GTCTC(1/5)
733 GTCTC
Bsp1407I 284 TGTACA T^GTACA
1125 TGTACA
BspLU11I 405 ACATGT A^CATGT
945 ACATGT
BsrGI 284 TGTACA T^GTACA
1125 TGTACA
BssKI 366 CCTGG ^CC[ACGT]GG
458 CCTGG
BstF5I 861 CATCC GGATG(2/0)
1155 CATCC
BstNI 366 CCTGG CC^[AT]GG
458 CCTGG
BstXI 74 CCATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
555 CCACACTTATGG
DpnII 799 GATC ^GATC
1001 GATC
DraI 177 TTTAAA TTT^AAA
195 TTTAAA
EaeI 230 CGGCCA [CT]^GGCC[GA]
1044 TGGCCA
EcoRII 366 CCTGG ^CC[AT]GG
458 CCTGG
Fnu4HI 717 GCAGC GC^[ACGT]GC
867 GCTGC
FokI 861 CATCC GGATG(9/13)
1155 CATCC
HapII 488 CCGG C^CGG
636 CCGG
HinfI 307 GACTC G^A[ACGT]TC
748 GATTC
HpaII 488 CCGG C^CGG
636 CCGG
HphI 77 TCACC GGTGA(8/7)
167 TCACC
ItaI 717 GCAGC GC^[ACGT]GC
867 GCTGC
MboI 799 GATC ^GATC
1001 GATC
MseI 178 TTAA T^TAA
196 TTAA
MspI 488 CCGG C^CGG
636 CCGG
MvaI 366 CCTGG CC^[AT]GG
458 CCTGG
NdeII 799 GATC ^GATC
1001 GATC
NspI 405 ACATGT [GA]CATG^[CT]
945 ACATGT
Sau3AI 799 GATC ^GATC
1001 GATC
ScrFI 366 CCTGG CC^[ACGT]GG
458 CCTGG
SfaNI 676 GATGC GCATC(5/9)
860 GCATC
SmlI 704 CTCAAG C^T[CT][GA]AG
794 CTCAAG
SspBI 284 TGTACA T^GTACA
1125 TGTACA
StuI 47 AGGCCT AGG^CCT
603 AGGCCT
Tru9I 178 TTAA T^TAA
196 TTAA
TseI 717 GCAGC G^C[AT]GC
867 GCTGC
TspRI 145 CAGTG CA[GC]TG[ACGT][ACGT]^
249 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BsrI Cac8I DdeI EarI HaeIII
Ksp632I MaeI MaeIII MboII MnlI
MslI MwoI NlaIII RsaI Tsp509I