Restriction map for
pFN21AE2405
(Circular) (Six-base) MAPSORT of: pFN21AE2405.seq Check: 1829 from: 1 to: 5884
pFN21AE2405 5884 bp
With 182 enzymes: SgfI *
February 28, 2012 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 3205 3205
Size: 5884
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2364 2661 3057 3947 5331 5599 1632
Size: 732 297 396 890 1384 268 1917
Fragments arranged by size:
1917 1384 890 732 396 297 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 2410 2950 4783 5760 1203
Size: 1207 540 1833 977 1327
Fragments arranged by size:
1833 1327 1207 977 540
AgeI A'CCGG_T
Cuts at: 4715 4715
Size: 5884
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 2882 1166
Size: 1716 4168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2888 1184
Size: 1704 4180
AlwNI CAG_nnn'CTG
Cuts at: 2155 3627 5199 2155
Size: 1472 1572 2840
Fragments arranged by size:
2840 1572 1472
ApaLI G'TGCA_C
Cuts at: 3941 5097 3941
Size: 1156 4728
ApoI r'AATT_y
Cuts at: 1493 2206 2510 2575 3193 3419 3571 5680 1493
Size: 713 304 65 618 226 152 2109 1697
Fragments arranged by size:
2109 1697 713 618 304 226 152 65
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 3209 1870
Size: 73 1266 4545
Fragments arranged by size:
4545 1266 73
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3205 4660 618
Size: 325 217 627 1418 1455 1842
Fragments arranged by size:
1842 1455 1418 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3203 1391
Size: 1812 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4913
BbvCI CC'TCA_GC
Cuts at: 2939 2939
Size: 5884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4075 4109 1903
Size: 34 2138 34 3678
Fragments arranged by size:
3678 2138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2202 3776 4986 2202
Size: 1574 1210 3100
Fragments arranged by size:
3100 1574 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5884
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4495 136
Size: 107 122 71 631 605 2823 1525
Fragments arranged by size:
2823 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5879 5879
Size: 5884
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 3945 5101 1790
Size: 2155 1156 2573
Fragments arranged by size:
2573 2155 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3615 4573 442
Size: 416 53 83 399 24 2198 958 1753
Fragments arranged by size:
2198 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2715 2713
Size: 2 5882
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2708 4544 1239
Size: 893 576 1836 2579
Fragments arranged by size:
2579 1836 893 576
Bpu10I CC'TnA_GC
Cuts at: 2939 2939
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2694 2784 4009 4874 5172 2694
Size: 90 1225 865 298 3406
Fragments arranged by size:
3406 1225 865 298 90
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4547 882
Size: 617 3048 2219
Fragments arranged by size:
3048 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3675 493
Size: 711 2471 2702
Fragments arranged by size:
2702 2471 711
BsaBI GATnn'nnATC
Cuts at: 5878 5878
Size: 5884
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3691 4073 4708 275
Size: 53 83 186 936 2158 382 635 1451
Fragments arranged by size:
2158 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2492 2640 4317 4715 4989 5136 5547 2492
Size: 148 1677 398 274 147 411 2829
Fragments arranged by size:
2829 1677 411 398 274 148 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5659
Fragments arranged by size:
5659 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2131 1186
Size: 945 4939
BseYI C'CCAG_C
Cuts at: 5087 5583 5087
Size: 496 5388
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2429 3945 4030 5101 2429
Size: 1516 85 1071 3212
Fragments arranged by size:
3212 1516 1071 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2429 3203 3945 4030 5101 1391
Size: 399 639 774 742 85 1071 2174
Fragments arranged by size:
2174 1071 774 742 639 399 85
BspEI T'CCGG_A
Cuts at: 5547 5547
Size: 5884
BspHI T'CATG_A
Cuts at: 2931 3773 5503 2931
Size: 842 1730 3312
Fragments arranged by size:
3312 1730 842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3239 844
Size: 2395 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4379 4553 65
Size: 1183 3131 174 1396
Fragments arranged by size:
3131 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4528 4715 1700
Size: 110 2718 187 2869
Fragments arranged by size:
2869 2718 187 110
BsrGI T'GTAC_A
Cuts at: 96 2289 3130 96
Size: 2193 841 2850
Fragments arranged by size:
2850 2193 841
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5884
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5884
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2567 2086
Size: 481 5403
BstYI r'GATC_y
Cuts at: 1972 2803 3214 3581 3966 3983 5435 5521 5533
Size: 831 411 367 385 17 1452 86 12
Cuts at: 5533 5879 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 831 411 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2164 2164
Size: 5884
BtgI C'CryG_G
Cuts at: 513 1065 2085 513
Size: 552 1020 4312
Fragments arranged by size:
4312 1020 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5145
Fragments arranged by size:
5145 683 56
BtsI GCAGTG_nn'
Cuts at: 3400 4194 4214 3400
Size: 794 20 5070
Fragments arranged by size:
5070 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 2184 2202 3188 2184
Size: 18 986 4880
Fragments arranged by size:
4880 986 18
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 3049 3331
Size: 54 1425 50 275 18 405 814 282
Cuts at: 3331 4220 5849 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 814 405 282 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2661 2775 2912 2930 3033 3814 1353
Size: 1308 114 137 18 103 781 3423
Fragments arranged by size:
3423 1308 781 137 114 103 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4471 4845 4991 387
Size: 1064 138 2882 374 146 1280
Fragments arranged by size:
2882 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2364 2661 2708 3057 3947 4544
Size: 393 500 232 297 47 349 890 597
Cuts at: 4544 5331 5599 1239
Size: 787 268 1524
Fragments arranged by size:
1524 890 787 597 500 393 349 297
268 232 47
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 5031 1678
Size: 3353 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 4525 4557 4599
Size: 32 135 32 520 32 2401 32 42
Cuts at: 4599 4631 1373
Size: 32 2626
Fragments arranged by size:
2626 2401 520 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2376 748
Size: 1628 4256
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KpnI G_GTAC'C
Cuts at: 3209 3209
Size: 5884
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2623 4998 5182 1305
Size: 1318 2375 184 2007
Fragments arranged by size:
2375 2007 1318 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3051 10
Size: 54 1425 343 1219 2843
Fragments arranged by size:
2843 1425 1219 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2255 2486 2565 2882 3842 4201
Size: 648 918 171 231 79 317 960 359
Cuts at: 4201 4360 518
Size: 159 2042
Fragments arranged by size:
2042 960 918 648 359 317 231 171
159 79
MspA1I CmG'CkG
Cuts at: 1606 3300 3977 5125 5370 1606
Size: 1694 677 1148 245 2120
Fragments arranged by size:
2120 1694 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5332
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 2414 2954 3242 4787 1132
Size: 97 1185 540 288 1545 2229
Fragments arranged by size:
2229 1545 1185 540 288 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5884
PciI A'CATG_T
Cuts at: 2410 2950 4783 2410
Size: 540 1833 3511
Fragments arranged by size:
3511 1833 540
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4982
PfoI T'CCnGG_A
Cuts at: 2370 2370
Size: 5884
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2400 2432 2400
Size: 32 5852
PstI C_TGCA'G
Cuts at: 830 3236 830
Size: 2406 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SapI GCTCTTCn'nnn_
Cuts at: 2930 2930
Size: 5884
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 2734 4132 5844 2734
Size: 1398 1712 2774
Fragments arranged by size:
2774 1712 1398
SfcI C'TryA_G
Cuts at: 826 1045 3232 4367 5048 5239 826
Size: 219 2187 1135 681 191 1471
Fragments arranged by size:
2187 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 3211 1872
Size: 1339 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2709 2799 3988 4889 5151 820
Size: 197 926 766 90 1189 901 262 1553
Fragments arranged by size:
1553 1189 926 901 766 262 197 90
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 2626 152
Size: 2474 3410
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 2054 2610 1517
Size: 537 556 4791
Fragments arranged by size:
4791 556 537
StyI C'CwwG_G
Cuts at: 513 1065 3323 513
Size: 552 2258 3074
Fragments arranged by size:
3074 2258 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3911 3928 4081 4266 4711 325
Size: 1255 2331 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 2416 2732
Size: 274 80 33 51 1443 312 127 316
Cuts at: 2732 2759 3096 3130 4130 5842 96
Size: 27 337 34 1000 1712 138
Fragments arranged by size:
1712 1443 1000 337 316 312 274 138
127 80 51 34 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2788 2820 5844 5876 2788
Size: 32 3024 32 2796
Fragments arranged by size:
3024 2796 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 3209 1870
Size: 1339 4545
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI