(Circular) (Six-base) MAPSORT of: pFN21AE9645.seq Check: 2788 from: 1 to: 12181 pFN21AE9645 12181 bp With 182 enzymes: SgfI * February 28, 2012 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7598 8575 7598 Size: 977 11204 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 9991 278 Size: 53 83 186 936 8455 2468 Fragments arranged by size: 8455 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 9130 9524 1937 Size: 7193 394 4594 Fragments arranged by size: 7193 4594 394 Acc65I G'GTAC_C Cuts at: 9502 9502 Size: 12181 AclI AA'CG_TT Cuts at: 1563 10308 10681 1563 Size: 8745 373 3063 Fragments arranged by size: 8745 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2112 2190 2208 3555 4236 4575 4656 4959 Size: 480 78 18 1347 681 339 81 303 Cuts at: 4959 4974 4992 5217 5511 5721 5862 6009 8322 Size: 15 18 225 294 210 141 147 2313 Cuts at: 8322 8711 8826 10244 11628 11896 1632 Size: 389 115 1418 1384 268 1917 Fragments arranged by size: 2313 1917 1418 1384 1347 681 480 389 339 303 294 268 225 210 147 141 115 81 78 18 18 15 AflII C'TTAA_G Cuts at: 820 1017 3179 6494 820 Size: 197 2162 3315 6507 Fragments arranged by size: 6507 3315 2162 197 AflIII A'CryG_T Cuts at: 1203 3976 4862 11080 12057 1203 Size: 2773 886 6218 977 1327 Fragments arranged by size: 6218 2773 1327 977 886 AgeI A'CCGG_T Cuts at: 3470 11012 3470 Size: 7542 4639 AhdI GACnn_n'nnGTC Cuts at: 2534 10910 2534 Size: 8376 3805 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 12181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4764 1184 Size: 3580 8601 AlwNI CAG_nnn'CTG Cuts at: 4175 4196 4259 5768 5987 7142 7748 8030 8557 Size: 21 63 1509 219 1155 606 282 527 Cuts at: 8557 9395 9924 11496 4175 Size: 838 529 1572 4860 Fragments arranged by size: 4860 1572 1509 1155 838 606 529 527 282 219 63 21 ApaLI G'TGCA_C Cuts at: 9426 10238 11394 9426 Size: 812 1156 10213 Fragments arranged by size: 10213 1156 812 ApoI r'AATT_y Cuts at: 1493 2335 2438 2860 3119 3244 4186 4272 4524 Size: 842 103 422 259 125 942 86 252 Cuts at: 4524 4603 4904 5076 5419 5644 5698 6441 6914 Size: 79 301 172 343 225 54 743 473 Cuts at: 6914 7210 7306 7478 7730 7879 8079 8306 9331 Size: 296 96 172 252 149 200 227 1025 Cuts at: 9331 9490 9716 9868 11977 1493 Size: 159 226 152 2109 1697 Fragments arranged by size: 2109 1697 1025 942 842 743 473 422 343 301 296 259 252 252 227 226 225 200 172 172 159 152 149 125 103 96 86 79 54 AseI AT'TA_AT Cuts at: 160 1032 4266 8376 8988 10735 160 Size: 872 3234 4110 612 1747 1606 Fragments arranged by size: 4110 3234 1747 1606 872 612 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12181 AvaI C'yCGr_G Cuts at: 1870 1943 7890 9506 1870 Size: 73 5947 1616 4545 Fragments arranged by size: 5947 4545 1616 73 BamHI G'GATC_C Cuts at: 7316 9511 9878 7316 Size: 2195 367 9619 Fragments arranged by size: 9619 2195 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 9502 10957 618 Size: 325 217 627 7715 1455 1842 Fragments arranged by size: 7715 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2019 4406 8724 8950 9500 1391 Size: 628 2387 4318 226 550 4072 Fragments arranged by size: 4318 4072 2387 628 550 226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 2418 2966 3243 3588 4437 4616 Size: 971 315 204 548 277 345 849 179 Cuts at: 4616 5703 7722 8192 928 Size: 1087 2019 470 4917 Fragments arranged by size: 4917 2019 1087 971 849 548 470 345 315 277 204 179 BbvCI CC'TCA_GC Cuts at: 5478 8037 5478 Size: 2559 9622 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 10372 10406 1903 Size: 34 8435 34 3678 Fragments arranged by size: 8435 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8677 8698 8747 10073 11283 8677 Size: 21 49 1326 1210 9575 Fragments arranged by size: 9575 1326 1210 49 21 BclI T'GATC_A Cuts at: 1548 3631 5638 7041 8575 9336 1548 Size: 2083 2007 1403 1534 761 4393 Fragments arranged by size: 4393 2083 2007 1534 1403 761 BfrBI ATG'CAT Cuts at: 2733 3427 6774 2733 Size: 694 3347 8140 Fragments arranged by size: 8140 3347 694 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 10792 136 Size: 107 122 71 631 605 9120 1525 Fragments arranged by size: 9120 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7886 12176 7886 Size: 4290 7891 BlpI GC'TnA_GC Cuts at: 2013 9598 2013 Size: 7585 4596 Bme1580I G_kGCm'C Cuts at: 1790 3051 4599 9430 10242 11398 1790 Size: 1261 1548 4831 812 1156 2573 Fragments arranged by size: 4831 2573 1548 1261 1156 812 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 12181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4429 8892 9311 Size: 416 53 83 399 24 3012 4463 419 Cuts at: 9311 9912 10870 442 Size: 601 958 1753 Fragments arranged by size: 4463 3012 1753 958 601 419 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4211 1056 Size: 3155 9026 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4684 8703 4684 Size: 4019 8162 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3091 6081 6744 7983 8996 9230 10841 1239 Size: 1852 2990 663 1239 1013 234 1611 2579 Fragments arranged by size: 2990 2579 1852 1611 1239 1013 663 234 Bpu10I CC'TnA_GC Cuts at: 4137 5064 5478 8037 8505 4137 Size: 927 414 2559 468 7813 Fragments arranged by size: 7813 2559 927 468 414 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3620 4015 6977 7059 7816 9074 10306 11171 11469 Size: 395 2962 82 757 1258 1232 865 298 Cuts at: 11469 3620 Size: 4332 Fragments arranged by size: 4332 2962 1258 1232 865 757 395 298 82 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3080 10844 882 Size: 617 1581 7764 2219 Fragments arranged by size: 7764 2219 1581 617 BsaAI yAC'GTr Cuts at: 493 1204 3977 9972 493 Size: 711 2773 5995 2702 Fragments arranged by size: 5995 2773 2702 711 BsaBI GATnn'nnATC Cuts at: 5637 9206 12175 5637 Size: 3569 2969 5643 Fragments arranged by size: 5643 3569 2969 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 9988 10370 11005 275 Size: 53 83 186 936 8455 382 635 1451 Fragments arranged by size: 8455 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3470 10614 11012 11286 11433 11844 3470 Size: 7144 398 274 147 411 3807 Fragments arranged by size: 7144 3807 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2089 2119 3089 3119 1178 Size: 30 165 30 686 30 970 30 10240 Fragments arranged by size: 10240 970 686 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2097 3732 4802 6356 6371 6374 6383 6386 Size: 911 1635 1070 1554 15 3 9 3 Cuts at: 6386 6389 6392 6401 6404 7243 1186 Size: 3 3 9 3 839 6124 Fragments arranged by size: 6124 1635 1554 1070 911 839 15 9 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 7993 11384 11880 7993 Size: 3391 496 8294 Fragments arranged by size: 8294 3391 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 2500 6674 7392 8262 1163 Size: 573 333 431 4174 718 870 5082 Fragments arranged by size: 5082 4174 870 718 573 431 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4004 9631 10392 11420 1540 Size: 275 186 2003 5627 761 1028 2301 Fragments arranged by size: 5627 2301 2003 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3423 4406 7417 8724 9430 10242 10327 11398 3423 Size: 983 3011 1307 706 812 85 1071 4206 Fragments arranged by size: 4206 3011 1307 1071 983 812 706 85 BsmI GAATG_Cn' Cuts at: 2733 6774 8050 9692 9785 2733 Size: 4041 1276 1642 93 5129 Fragments arranged by size: 5129 4041 1642 1276 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6505 6505 Size: 12181 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 3051 3423 4406 4599 7417 8724 Size: 399 229 1032 372 983 193 2818 1307 Cuts at: 8724 8950 9430 9500 10242 10327 11398 1391 Size: 226 480 70 742 85 1071 2174 Fragments arranged by size: 2818 2174 1307 1071 1032 983 742 480 399 372 229 226 193 85 70 BspEI T'CCGG_A Cuts at: 11844 11844 Size: 12181 BspHI T'CATG_A Cuts at: 2059 9312 9339 10070 11800 2059 Size: 7253 27 731 1730 2440 Fragments arranged by size: 7253 2440 1730 731 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3529 6646 7598 8575 9536 844 Size: 2685 3117 952 977 961 3489 Fragments arranged by size: 3489 3117 2685 977 961 952 BsrBI CCG'CTC Cuts at: 1394 1823 10068 1394 Size: 429 8245 3507 Fragments arranged by size: 8245 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 2576 8964 10676 10850 65 Size: 1183 879 449 6388 1712 174 1396 Fragments arranged by size: 6388 1712 1396 1183 879 449 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3470 10825 11012 1700 Size: 110 1660 7355 187 2869 Fragments arranged by size: 7355 2869 1660 187 110 BsrGI T'GTAC_A Cuts at: 96 2386 96 Size: 2290 9891 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12181 BssSI C'ACGA_G Cuts at: 10241 11253 10241 Size: 1012 11169 BstAPI GCAn_nnn'nTGC Cuts at: 794 2365 2730 794 Size: 1571 365 10245 Fragments arranged by size: 10245 1571 365 BstBI TT'CG_AA Cuts at: 3964 8086 8239 10985 3964 Size: 4122 153 2746 5160 Fragments arranged by size: 5160 4122 2746 153 BstEII G'GTnAC_C Cuts at: 1184 9305 1184 Size: 8121 4060 BstXI CCAn_nnnn'nTGG Cuts at: 2773 2773 Size: 12181 BstYI r'GATC_y Cuts at: 1972 3968 5873 7316 7886 8363 9511 9878 10263 Size: 1996 1905 1443 570 477 1148 367 385 Cuts at: 10263 10280 11732 11818 11830 12176 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1996 1977 1905 1452 1443 1148 570 477 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5091 6990 5091 Size: 1899 10282 BtgI C'CryG_G Cuts at: 513 1065 7383 8514 513 Size: 552 6318 1131 4180 Fragments arranged by size: 6318 4180 1131 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3275 4941 5682 487 Size: 683 56 2049 1666 741 6986 Fragments arranged by size: 6986 2049 1666 741 683 56 BtsI GCAGTG_nn' Cuts at: 2517 2835 3658 6136 7852 9042 9697 10491 10511 Size: 318 823 2478 1716 1190 655 794 20 Cuts at: 10511 2517 Size: 4187 Fragments arranged by size: 4187 2478 1716 1190 823 794 655 318 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2648 2683 529 Size: 35 2084 35 10027 Fragments arranged by size: 10027 2084 35 35 DraI TTT'AAA Cuts at: 2428 3126 4305 7947 9485 2428 Size: 698 1179 3642 1538 5124 Fragments arranged by size: 5124 3642 1538 1179 698 DraIII CAC_nnn'GTG Cuts at: 6594 6594 Size: 12181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 11188 809 Size: 507 9872 1802 Fragments arranged by size: 9872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 9628 10517 12146 Size: 54 1425 50 275 18 7798 889 1629 Cuts at: 12146 8 Size: 43 Fragments arranged by size: 7798 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 9628 1537 Size: 275 7816 4090 Fragments arranged by size: 7816 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2209 4555 4675 5236 5306 5380 6333 Size: 831 25 2346 120 561 70 74 953 Cuts at: 6333 6348 6360 6378 6495 6501 7085 7591 8672 Size: 15 12 18 117 6 584 506 1081 Cuts at: 8672 8790 8992 9157 10111 1353 Size: 118 202 165 954 3423 Fragments arranged by size: 3423 2346 1081 954 953 831 584 561 506 202 165 120 118 117 74 70 25 18 15 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 10768 11142 11288 387 Size: 1064 138 9179 374 146 1280 Fragments arranged by size: 9179 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4404 8722 4404 Size: 4318 7863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2190 2208 3091 3555 4236 4575 Size: 393 480 78 18 883 464 681 339 Cuts at: 4575 4656 4959 4974 4992 5217 5511 5721 5862 Size: 81 303 15 18 225 294 210 141 Cuts at: 5862 6009 6081 6744 7983 8322 8711 8826 8996 Size: 147 72 663 1239 339 389 115 170 Cuts at: 8996 9230 10244 10841 11628 11896 1239 Size: 234 1014 597 787 268 1524 Fragments arranged by size: 1524 1239 1014 883 787 681 663 597 480 464 393 389 339 339 303 294 268 234 225 210 170 147 141 115 81 78 72 18 18 15 EcoNI CCTnn'n_nnAGG Cuts at: 4220 6426 9465 4220 Size: 2206 3039 6936 Fragments arranged by size: 6936 3039 2206 EcoO109I rG'GnC_Cy Cuts at: 2432 7139 8980 9458 2432 Size: 4707 1841 478 5155 Fragments arranged by size: 5155 4707 1841 478 EcoRI G'AATT_C Cuts at: 4524 5644 7210 7879 9490 9868 4524 Size: 1120 1566 669 1611 378 6837 Fragments arranged by size: 6837 1611 1566 1120 669 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2513 2620 3410 5870 8180 2513 Size: 107 790 2460 2310 6514 Fragments arranged by size: 6514 2460 2310 790 107 FspI TGC'GCA Cuts at: 10687 10687 Size: 12181 HaeII r_GCGC'y Cuts at: 1678 11328 1678 Size: 9650 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2520 2552 3542 3574 3665 Size: 32 135 32 948 32 990 32 91 Cuts at: 3665 3697 4165 4197 4532 4564 4831 4863 4871 Size: 32 468 32 335 32 267 32 8 Cuts at: 4871 4903 5168 5200 5312 5344 5591 5623 5858 Size: 32 265 32 112 32 247 32 235 Cuts at: 5858 5890 6041 6073 6401 6433 7742 7774 7814 Size: 32 151 32 328 32 1309 32 40 Cuts at: 7814 7846 8328 8360 8518 8550 9034 9066 9156 Size: 32 482 32 158 32 484 32 90 Cuts at: 9156 9188 9328 9360 10822 10854 10896 10928 1373 Size: 32 140 32 1462 32 42 32 2626 Fragments arranged by size: 2626 1462 1309 990 948 484 482 468 335 328 267 265 247 235 158 151 140 135 112 91 90 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 2905 5205 8802 9525 9771 669 Size: 988 281 967 2300 3597 723 246 3079 Fragments arranged by size: 3597 3079 2300 988 967 723 281 246 HindIII A'AGCT_T Cuts at: 748 2054 4772 6953 7122 7616 748 Size: 1306 2718 2181 169 494 5313 Fragments arranged by size: 5313 2718 2181 1306 494 169 HpaI GTT'AAC Cuts at: 9771 9771 Size: 12181 KpnI G_GTAC'C Cuts at: 9506 9506 Size: 12181 MfeI C'AATT_G Cuts at: 2256 9780 2256 Size: 7524 4657 MluI A'CGCG_T Cuts at: 12057 12057 Size: 12181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2805 3464 6219 7076 11295 11479 1305 Size: 1500 659 2755 857 4219 184 2007 Fragments arranged by size: 4219 2755 2007 1500 857 659 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 10359 Fragments arranged by size: 10359 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2771 3432 7500 8763 10139 10498 10657 Size: 648 1605 661 4068 1263 1376 359 159 Cuts at: 10657 518 Size: 2042 Fragments arranged by size: 4068 2042 1605 1376 1263 661 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 6205 6253 8974 9071 9213 9597 10274 Size: 406 4193 48 2721 97 142 384 677 Cuts at: 10274 11422 11667 1606 Size: 1148 245 2120 Fragments arranged by size: 4193 2721 2120 1148 677 406 384 245 142 97 48 NcoI C'CATG_G Cuts at: 513 1065 7383 8514 513 Size: 552 6318 1131 4180 Fragments arranged by size: 6318 4180 1131 552 NdeI CA'TA_TG Cuts at: 387 5263 387 Size: 4876 7305 NheI G'CTAG_C Cuts at: 1052 4207 1052 Size: 3155 9026 NotI GC'GGCC_GC Cuts at: 9628 9628 Size: 12181 NsiI A_TGCA'T Cuts at: 2735 3429 6776 2735 Size: 694 3347 8140 Fragments arranged by size: 8140 3347 694 NspI r_CATG'y Cuts at: 1132 1229 2844 3427 4866 7009 9539 11084 1132 Size: 97 1615 583 1439 2143 2530 1545 2229 Fragments arranged by size: 2530 2229 2143 1615 1545 1439 583 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 12181 PciI A'CATG_T Cuts at: 4862 11080 4862 Size: 6218 5963 PflMI CCAn_nnn'nTGG Cuts at: 1108 6790 9395 1108 Size: 5682 2605 3894 Fragments arranged by size: 5682 3894 2605 PfoI T'CCnGG_A Cuts at: 6421 9454 6421 Size: 3033 9148 PmeI GTTT'AAAC Cuts at: 9485 9485 Size: 12181 PmlI CAC'GTG Cuts at: 3977 3977 Size: 12181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4228 4260 10250 10282 11763 11795 4228 Size: 32 5990 32 1481 32 4614 Fragments arranged by size: 5990 4614 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 8980 9458 8980 Size: 478 11703 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12181 PsiI TTA'TAA Cuts at: 7346 8217 9751 7346 Size: 871 1534 9776 Fragments arranged by size: 9776 1534 871 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6690 6722 6690 Size: 32 12149 PstI C_TGCA'G Cuts at: 830 7014 9533 830 Size: 6184 2519 3478 Fragments arranged by size: 6184 3478 2519 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12181 PvuII CAG'CTG Cuts at: 8974 9071 9213 8974 Size: 97 142 11942 Fragments arranged by size: 11942 142 97 SacI G_AGCT'C Cuts at: 4406 8724 4406 Size: 4318 7863 SalI G'TCGA_C Cuts at: 1936 9523 1936 Size: 7587 4594 SapI GCTCTTCn'nnn_ Cuts at: 2209 2209 Size: 12181 SbfI CC_TGCA'GG Cuts at: 9533 9533 Size: 12181 ScaI AGT'ACT Cuts at: 7550 10429 12141 7550 Size: 2879 1712 7590 Fragments arranged by size: 7590 2879 1712 SfcI C'TryA_G Cuts at: 826 1045 3738 4125 5649 7010 9529 10664 11345 Size: 219 2693 387 1524 1361 2519 1135 681 Cuts at: 11345 11536 826 Size: 191 1471 Fragments arranged by size: 2693 2519 1524 1471 1361 1135 681 387 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12181 SmaI CCC'GGG Cuts at: 1872 9508 1872 Size: 7636 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3179 3599 3994 6494 6956 7074 Size: 197 926 1236 420 395 2500 462 118 Cuts at: 7074 7831 9053 10285 11186 11448 820 Size: 757 1222 1232 901 262 1553 Fragments arranged by size: 2500 1553 1236 1232 1222 926 901 757 462 420 395 262 197 118 SnaBI TAC'GTA Cuts at: 493 9972 493 Size: 9479 2702 SpeI A'CTAG_T Cuts at: 152 2963 152 Size: 2811 9370 SphI G_CATG'C Cuts at: 1132 9539 1132 Size: 8407 3774 SspI AAT'ATT Cuts at: 5 52 3697 5348 7113 10105 5 Size: 47 3645 1651 1765 2992 2081 Fragments arranged by size: 3645 2992 2081 1765 1651 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 12181 StyI C'CwwG_G Cuts at: 513 1065 2746 6796 7383 7661 8514 9466 9620 Size: 552 1681 4050 587 278 853 952 154 Cuts at: 9620 513 Size: 3074 Fragments arranged by size: 4050 3074 1681 952 853 587 552 278 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2826 4018 6601 10208 10225 10378 10563 Size: 1255 1246 1192 2583 3607 17 153 185 Cuts at: 10563 11008 325 Size: 445 1498 Fragments arranged by size: 3607 2583 1498 1255 1246 1192 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2386 4873 7548 Size: 274 80 33 51 1443 409 2487 2675 Cuts at: 7548 9109 9255 10427 12139 96 Size: 1561 146 1172 1712 138 Fragments arranged by size: 2675 2487 1712 1561 1443 1172 409 274 146 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6580 6612 7071 7103 12141 12173 6580 Size: 32 459 32 5038 32 6588 Fragments arranged by size: 6588 5038 459 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2831 1316 Size: 1515 10666 XbaI T'CTAG_A Cuts at: 9517 9517 Size: 12181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6698 8001 6698 Size: 1303 10878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 12181 XmaI C'CCGG_G Cuts at: 1870 9506 1870 Size: 7636 4545 XmnI GAAnn'nnTTC Cuts at: 4351 5302 7522 8082 8160 10310 4351 Size: 951 2220 560 78 2150 6222 Fragments arranged by size: 6222 2220 2150 951 560 78 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 9989 276 Size: 53 83 186 936 8455 2468 Fragments arranged by size: 8455 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI