Restriction map for
pFN21AE9645
(Linear) (Six-base) MAPSORT of: KIEE9645.seq Check: 3772 from: 1 to: 7476
KIEE9645.orf
With 182 enzymes: SgfI *
May 29, 2012 11:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5593 6570 7476
Size: 5593 977 906
Fragments arranged by size:
5593 977 906
AccI GT'mk_AC
Cuts at: 0 7125 7476
Size: 7125 351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 185 203 1550 2231 2570 2651 2954
Size: 107 78 18 1347 681 339 81 303
Cuts at: 2954 2969 2987 3212 3506 3716 3857 4004 6317
Size: 15 18 225 294 210 141 147 2313
Cuts at: 6317 6706 6821 7476
Size: 389 115 655
Fragments arranged by size:
2313 1347 681 655 389 339 303 294
225 210 147 141 115 107 81 78
18 18 15
AflII C'TTAA_G
Cuts at: 0 1174 4489 7476
Size: 1174 3315 2987
Fragments arranged by size:
3315 2987 1174
AflIII A'CryG_T
Cuts at: 0 1971 2857 7476
Size: 1971 886 4619
Fragments arranged by size:
4619 1971 886
AgeI A'CCGG_T
Cuts at: 0 1465 7476
Size: 1465 6011
AhdI GACnn_n'nnGTC
Cuts at: 0 529 7476
Size: 529 6947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2759 7476
Size: 2759 4717
AlwNI CAG_nnn'CTG
Cuts at: 0 2170 2191 2254 3763 3982 5137 5743 6025
Size: 2170 21 63 1509 219 1155 606 282
Cuts at: 6025 6552 7390 7476
Size: 527 838 86
Fragments arranged by size:
2170 1509 1155 838 606 527 282 219
86 63 21
ApaLI G'TGCA_C
Cuts at: 0 7421 7476
Size: 7421 55
ApoI r'AATT_y
Cuts at: 0 330 433 855 1114 1239 2181 2267 2519
Size: 330 103 422 259 125 942 86 252
Cuts at: 2519 2598 2899 3071 3414 3639 3693 4436 4909
Size: 79 301 172 343 225 54 743 473
Cuts at: 4909 5205 5301 5473 5725 5874 6074 6301 7326
Size: 296 96 172 252 149 200 227 1025
Cuts at: 7326 7476
Size: 150
Fragments arranged by size:
1025 942 743 473 422 343 330 301
296 259 252 252 227 225 200 172
172 150 149 125 103 96 86 79
54
AseI AT'TA_AT
Cuts at: 0 2261 6371 6983 7476
Size: 2261 4110 612 493
Fragments arranged by size:
4110 2261 612 493
AvaI C'yCGr_G
Cuts at: 0 5885 7476
Size: 5885 1591
BamHI G'GATC_C
Cuts at: 0 5311 7476
Size: 5311 2165
BanII G_rGCy'C
Cuts at: 0 14 2401 6719 6945 7476
Size: 14 2387 4318 226 531
Fragments arranged by size:
4318 2387 531 226 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 413 961 1238 1583 2432 2611 3698
Size: 209 204 548 277 345 849 179 1087
Cuts at: 3698 5717 6187 7476
Size: 2019 470 1289
Fragments arranged by size:
2019 1289 1087 849 548 470 345 277
209 204 179
BbvCI CC'TCA_GC
Cuts at: 0 3473 6032 7476
Size: 3473 2559 1444
Fragments arranged by size:
3473 2559 1444
BciVI GTATCCnnnnn_n'
Cuts at: 0 6672 6693 6742 7476
Size: 6672 21 49 734
Fragments arranged by size:
6672 734 49 21
BclI T'GATC_A
Cuts at: 0 1626 3633 5036 6570 7331 7476
Size: 1626 2007 1403 1534 761 145
Fragments arranged by size:
2007 1626 1534 1403 761 145
BfrBI ATG'CAT
Cuts at: 0 728 1422 4769 7476
Size: 728 694 3347 2707
Fragments arranged by size:
3347 2707 728 694
BglII A'GATC_T
Cuts at: 0 5881 7476
Size: 5881 1595
BlpI GC'TnA_GC
Cuts at: 0 8 7476
Size: 8 7468
Bme1580I G_kGCm'C
Cuts at: 0 1046 2594 7425 7476
Size: 1046 1548 4831 51
Fragments arranged by size:
4831 1548 1046 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 2424 6887 7306 7476
Size: 2424 4463 419 170
Fragments arranged by size:
4463 2424 419 170
BmtI G_CTAG'C
Cuts at: 0 2206 7476
Size: 2206 5270
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2679 6698 7476
Size: 2679 4019 778
Fragments arranged by size:
4019 2679 778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 4076 4739 5978 6991 7225 7476
Size: 1086 2990 663 1239 1013 234 251
Fragments arranged by size:
2990 1239 1086 1013 663 251 234
Bpu10I CC'TnA_GC
Cuts at: 0 2132 3059 3473 6032 6500 7476
Size: 2132 927 414 2559 468 976
Fragments arranged by size:
2559 2132 976 927 468 414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1615 2010 4972 5054 5811 7069 7476
Size: 1615 395 2962 82 757 1258 407
Fragments arranged by size:
2962 1615 1258 757 407 395 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 1075 7476
Size: 1075 6401
BsaAI yAC'GTr
Cuts at: 0 1972 7476
Size: 1972 5504
BsaBI GATnn'nnATC
Cuts at: 0 3632 7201 7476
Size: 3632 3569 275
Fragments arranged by size:
3632 3569 275
BsaWI w'CCGG_w
Cuts at: 0 1465 7476
Size: 1465 6011
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 84 114 1084 1114 7476
Size: 84 30 970 30 6362
Fragments arranged by size:
6362 970 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 92 1727 2797 4351 4366 4369 4378 4381
Size: 92 1635 1070 1554 15 3 9 3
Cuts at: 4381 4384 4387 4396 4399 5238 7476
Size: 3 3 9 3 839 2238
Fragments arranged by size:
2238 1635 1554 1070 839 92 15 9
9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 5988 7476
Size: 5988 1488
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 495 4669 5387 6257 7476
Size: 64 431 4174 718 870 1219
Fragments arranged by size:
4174 1219 870 718 431 64
BsiEI CG_ry'CG
Cuts at: 0 1999 7476
Size: 1999 5477
BsiHKAI G_wGCw'C
Cuts at: 0 1418 2401 5412 6719 7425 7476
Size: 1418 983 3011 1307 706 51
Fragments arranged by size:
3011 1418 1307 983 706 51
BsmI GAATG_Cn'
Cuts at: 0 728 4769 6045 7476
Size: 728 4041 1276 1431
Fragments arranged by size:
4041 1431 1276 728
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4500 7476
Size: 4500 2976
Bsp1286I G_dGCh'C
Cuts at: 0 14 1046 1418 2401 2594 5412 6719 6945
Size: 14 1032 372 983 193 2818 1307 226
Cuts at: 6945 7425 7476
Size: 480 51
Fragments arranged by size:
2818 1307 1032 983 480 372 226 193
51 14
BspHI T'CATG_A
Cuts at: 0 54 7307 7334 7476
Size: 54 7253 27 142
Fragments arranged by size:
7253 142 54 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1524 4641 5593 6570 7476
Size: 1524 3117 952 977 906
Fragments arranged by size:
3117 1524 977 952 906
BsrDI GCAATG_nn'
Cuts at: 0 122 571 6959 7476
Size: 122 449 6388 517
Fragments arranged by size:
6388 517 449 122
BsrFI r'CCGG_y
Cuts at: 0 1465 7476
Size: 1465 6011
BsrGI T'GTAC_A
Cuts at: 0 381 7476
Size: 381 7095
BstAPI GCAn_nnn'nTGC
Cuts at: 0 360 725 7476
Size: 360 365 6751
Fragments arranged by size:
6751 365 360
BstBI TT'CG_AA
Cuts at: 0 1959 6081 6234 7476
Size: 1959 4122 153 1242
Fragments arranged by size:
4122 1959 1242 153
BstEII G'GTnAC_C
Cuts at: 0 7300 7476
Size: 7300 176
BstXI CCAn_nnnn'nTGG
Cuts at: 0 768 7476
Size: 768 6708
BstYI r'GATC_y
Cuts at: 0 1963 3868 5311 5881 6358 7476
Size: 1963 1905 1443 570 477 1118
Fragments arranged by size:
1963 1905 1443 1118 570 477
Bsu36I CC'TnA_GG
Cuts at: 0 3086 4985 7476
Size: 3086 1899 2491
Fragments arranged by size:
3086 2491 1899
BtgI C'CryG_G
Cuts at: 0 5378 6509 7476
Size: 5378 1131 967
Fragments arranged by size:
5378 1131 967
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1270 2936 3677 7476
Size: 1270 1666 741 3799
Fragments arranged by size:
3799 1666 1270 741
BtsI GCAGTG_nn'
Cuts at: 0 512 830 1653 4131 5847 7037 7476
Size: 512 318 823 2478 1716 1190 439
Fragments arranged by size:
2478 1716 1190 823 512 439 318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 643 678 7476
Size: 643 35 6798
Fragments arranged by size:
6798 643 35
DraI TTT'AAA
Cuts at: 0 423 1121 2300 5942 7476
Size: 423 698 1179 3642 1534
Fragments arranged by size:
3642 1534 1179 698 423
DraIII CAC_nnn'GTG
Cuts at: 0 4589 7476
Size: 4589 2887
EarI CTCTTCn'nnn_
Cuts at: 0 179 204 2550 2670 3231 3301 3375 4328
Size: 179 25 2346 120 561 70 74 953
Cuts at: 4328 4343 4355 4373 4490 4496 5080 5586 6667
Size: 15 12 18 117 6 584 506 1081
Cuts at: 6667 6785 6987 7152 7476
Size: 118 202 165 324
Fragments arranged by size:
2346 1081 953 584 561 506 324 202
179 165 120 118 117 74 70 25
18 15 12 6
EcoICRI GAG'CTC
Cuts at: 0 2399 6717 7476
Size: 2399 4318 759
Fragments arranged by size:
4318 2399 759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 185 203 1086 1550 2231 2570 2651
Size: 107 78 18 883 464 681 339 81
Cuts at: 2651 2954 2969 2987 3212 3506 3716 3857 4004
Size: 303 15 18 225 294 210 141 147
Cuts at: 4004 4076 4739 5978 6317 6706 6821 6991 7225
Size: 72 663 1239 339 389 115 170 234
Cuts at: 7225 7476
Size: 251
Fragments arranged by size:
1239 883 681 663 464 389 339 339
303 294 251 234 225 210 170 147
141 115 107 81 78 72 18 18
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2215 4421 7460 7476
Size: 2215 2206 3039 16
Fragments arranged by size:
3039 2215 2206 16
EcoO109I rG'GnC_Cy
Cuts at: 0 427 5134 6975 7453 7476
Size: 427 4707 1841 478 23
Fragments arranged by size:
4707 1841 478 427 23
EcoRI G'AATT_C
Cuts at: 0 2519 3639 5205 5874 7476
Size: 2519 1120 1566 669 1602
Fragments arranged by size:
2519 1602 1566 1120 669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 508 615 1405 3865 6175 7476
Size: 508 107 790 2460 2310 1301
Fragments arranged by size:
2460 2310 1301 790 508 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 515 547 1537 1569 1660 1692 2160 2192
Size: 515 32 990 32 91 32 468 32
Cuts at: 2192 2527 2559 2826 2858 2866 2898 3163 3195
Size: 335 32 267 32 8 32 265 32
Cuts at: 3195 3307 3339 3586 3618 3853 3885 4036 4068
Size: 112 32 247 32 235 32 151 32
Cuts at: 4068 4396 4428 5737 5769 5809 5841 6323 6355
Size: 328 32 1309 32 40 32 482 32
Cuts at: 6355 6513 6545 7029 7061 7151 7183 7323 7355
Size: 158 32 484 32 90 32 140 32
Cuts at: 7355 7476
Size: 121
Fragments arranged by size:
1309 990 515 484 482 468 335 328
267 265 247 235 158 151 140 121
112 91 90 40 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
8
HincII GTy'rAC
Cuts at: 0 900 3200 6797 7476
Size: 900 2300 3597 679
Fragments arranged by size:
3597 2300 900 679
HindIII A'AGCT_T
Cuts at: 0 49 2767 4948 5117 5611 7476
Size: 49 2718 2181 169 494 1865
Fragments arranged by size:
2718 2181 1865 494 169 49
MfeI C'AATT_G
Cuts at: 0 251 7476
Size: 251 7225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 1459 4214 5071 7476
Size: 800 659 2755 857 2405
Fragments arranged by size:
2755 2405 857 800 659
MslI CAynn'nnrTG
Cuts at: 0 766 1427 5495 6758 7476
Size: 766 661 4068 1263 718
Fragments arranged by size:
4068 1263 766 718 661
MspA1I CmG'CkG
Cuts at: 0 7 4200 4248 6969 7066 7208 7476
Size: 7 4193 48 2721 97 142 268
Fragments arranged by size:
4193 2721 268 142 97 48 7
NcoI C'CATG_G
Cuts at: 0 5378 6509 7476
Size: 5378 1131 967
Fragments arranged by size:
5378 1131 967
NdeI CA'TA_TG
Cuts at: 0 3258 7476
Size: 3258 4218
NheI G'CTAG_C
Cuts at: 0 2202 7476
Size: 2202 5274
NsiI A_TGCA'T
Cuts at: 0 730 1424 4771 7476
Size: 730 694 3347 2705
Fragments arranged by size:
3347 2705 730 694
NspI r_CATG'y
Cuts at: 0 839 1422 2861 5004 7476
Size: 839 583 1439 2143 2472
Fragments arranged by size:
2472 2143 1439 839 583
PciI A'CATG_T
Cuts at: 0 2857 7476
Size: 2857 4619
PflMI CCAn_nnn'nTGG
Cuts at: 0 4785 7390 7476
Size: 4785 2605 86
Fragments arranged by size:
4785 2605 86
PfoI T'CCnGG_A
Cuts at: 0 4416 7449 7476
Size: 4416 3033 27
Fragments arranged by size:
4416 3033 27
PmlI CAC'GTG
Cuts at: 0 1972 7476
Size: 1972 5504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2223 2255 7476
Size: 2223 32 5221
Fragments arranged by size:
5221 2223 32
PpuMI rG'GwC_Cy
Cuts at: 0 6975 7453 7476
Size: 6975 478 23
Fragments arranged by size:
6975 478 23
PsiI TTA'TAA
Cuts at: 0 5341 6212 7476
Size: 5341 871 1264
Fragments arranged by size:
5341 1264 871
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4685 4717 7476
Size: 4685 32 2759
Fragments arranged by size:
4685 2759 32
PstI C_TGCA'G
Cuts at: 0 5009 7476
Size: 5009 2467
PvuII CAG'CTG
Cuts at: 0 6969 7066 7208 7476
Size: 6969 97 142 268
Fragments arranged by size:
6969 268 142 97
SacI G_AGCT'C
Cuts at: 0 2401 6719 7476
Size: 2401 4318 757
Fragments arranged by size:
4318 2401 757
SapI GCTCTTCn'nnn_
Cuts at: 0 204 7476
Size: 204 7272
ScaI AGT'ACT
Cuts at: 0 5545 7476
Size: 5545 1931
SfcI C'TryA_G
Cuts at: 0 1733 2120 3644 5005 7476
Size: 1733 387 1524 1361 2471
Fragments arranged by size:
2471 1733 1524 1361 387
SmlI C'TyrA_G
Cuts at: 0 1174 1594 1989 4489 4951 5069 5826 7048
Size: 1174 420 395 2500 462 118 757 1222
Cuts at: 7048 7476
Size: 428
Fragments arranged by size:
2500 1222 1174 757 462 428 420 395
118
SpeI A'CTAG_T
Cuts at: 0 958 7476
Size: 958 6518
SspI AAT'ATT
Cuts at: 0 1692 3343 5108 7476
Size: 1692 1651 1765 2368
Fragments arranged by size:
2368 1765 1692 1651
StyI C'CwwG_G
Cuts at: 0 741 4791 5378 5656 6509 7461 7476
Size: 741 4050 587 278 853 952 15
Fragments arranged by size:
4050 952 853 741 587 278 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 821 2013 4596 7476
Size: 821 1192 2583 2880
Fragments arranged by size:
2880 2583 1192 821
TatI w'GTAC_w
Cuts at: 0 381 2868 5543 7104 7250 7476
Size: 381 2487 2675 1561 146 226
Fragments arranged by size:
2675 2487 1561 381 226 146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4575 4607 5066 5098 7476
Size: 4575 32 459 32 2378
Fragments arranged by size:
4575 2378 459 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 826 7476
Size: 826 6650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4693 5996 7476
Size: 4693 1303 1480
Fragments arranged by size:
4693 1480 1303
XmnI GAAnn'nnTTC
Cuts at: 0 2346 3297 5517 6077 6155 7476
Size: 2346 951 2220 560 78 1321
Fragments arranged by size:
2346 2220 1321 951 560 78
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AvaI BamHI BanII BbsI
BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII HindIII MfeI MmeI MslI
MspA1I NcoI NdeI NheI NsiI NspI PciI PflMI
PfoI PmlI PpiI PpuMI PsiI PsrI PstI PvuII
SacI SapI ScaI SfcI SmlI SpeI SspI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AleI AloI ApaI AscI
AsiSI AvrII BaeI BanI BbeI BcgI BglI BmgBI
BsaHI BsiWI BspEI BsrBI BssHII BssSI BstZ17I ClaI
DrdI EaeI EagI EciI EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MluI MscI NaeI NarI
NgoMIV NotI NruI PacI PasI PmeI PshAI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI XbaI XhoI XmaI ZraI