################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccIII AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII BbeI BciVI BclI BfrI BlpI Bpu1102I BsaBI BsaHI BseAI BsiWI BsmBI BspEI BspLU11I BssHII BssSI BstBI BstXI CelII CpoI DrdI Eco47III EcoT22I FbaI FspI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NheI NotI NsiI NspV PacI PaeR7I PinAI PmeI PshBI RsrII ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AclI 3315 AACGTT AA^CGTT AocI 2567 CCTCAGG CC^T[ACGT]AGG AvrII 907 CCTAGG C^CTAGG BalI 3788 TGGCCA TGG^CCA BamHI 2179 GGATCC G^GATCC BbrPI 2479 CACGTG CAC^GTG BbvCI 4111 CCTCAGC CCTCAGC(-5/-2) BglI 3302 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 845 AGATCT A^GATCT BlnI 907 CCTAGG C^CTAGG BsaAI 2479 CACGTG [CT]AC^GT[GA] BsaWI 2880 ACCGGA [AT]^CCGG[AT] BspDI 2339 ATCGAT AT^CGAT BspHI 2498 TCATGA T^CATGA Bst1107I 1557 GTATAC GTA^TAC BstAPI 3850 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 765 GGTCACC G^GT[ACGT]ACC BstPI 765 GGTCACC G^GT[ACGT]ACC BstZ17I 1557 GTATAC GTA^TAC Bsu36I 2567 CCTCAGG CC^T[ACGT]AGG ClaI 2339 ATCGAT AT^CGAT DraI 1932 TTTAAA TTT^AAA Eco81I 2567 CCTCAGG CC^T[ACGT]AGG EcoO65I 765 GGTCACC G^GT[ACGT]ACC EcoRI 3441 GAATTC G^AATTC EcoRV 214 GATATC GAT^ATC FseI 4118 GGCCGGCC GGCCGG^CC HincII 1149 GTCGAC GT[CT]^[GA]AC HindII 1149 GTCGAC GT[CT]^[GA]AC KspI 1852 CCGCGG CCGC^GG MluNI 3788 TGGCCA TGG^CCA MscI 3788 TGGCCA TGG^CCA NaeI 4119 GCCGGC GCC^GGC NdeI 2332 CATATG CA^TATG NgoMIV 4119 GCCGGC G^CCGGC NruI 780 TCGCGA TCG^CGA PmaCI 2479 CACGTG CAC^GTG PmlI 2479 CACGTG CAC^GTG PshAI 695 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3315 AACGTT AA^CGTT PvuI 2341 CGATCG CGAT^CG PvuII 3446 CAGCTG CAG^CTG RcaI 2498 TCATGA T^CATGA SacII 1852 CCGCGG CCGC^GG SalI 1149 GTCGAC G^TCGAC SbfI 3474 CCTGCAGG CCTGCA^GG SexAI 2952 ACCAGGT A^CC[AT]GGT SmaI 2032 CCCGGG CCC^GGG Sse8387I 3474 CCTGCAGG CCTGCA^GG XbaI 1338 TCTAGA T^CTAGA XmaCI 2032 CCCGGG C^CCGGG XmaI 2032 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspI 382 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1682 GACGGAGTC AvaI 2032 CCCGGG C^[CT]CG[GA]G 2492 CCCGAG BsoBI 2032 CCCGGG C^[CT]CG[GA]G 2492 CCCGAG Bsp1407I 491 TGTACA T^GTACA 4322 TGTACA BsrDI 1600 GCAATG GCAATG(2/0) 3112 GCAATG BsrFI 368 ACCGGC [GA]^CCGG[CT] 4119 GCCGGC BsrGI 491 TGTACA T^GTACA 4322 TGTACA Cfr10I 368 ACCGGC [GA]^CCGG[CT] 4119 GCCGGC DraIII 2951 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 4075 CACAGTGTG EagI 23 CGGCCG C^GGCCG 4117 CGGCCG EclXI 23 CGGCCG C^GGCCG 4117 CGGCCG Eco52I 23 CGGCCG C^GGCCG 4117 CGGCCG HaeII 1806 AGCGCC [GA]GCGC^[CT] 3208 GGCGCT PflFI 382 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1682 GACGGAGTC SmlI 570 CTCAAG C^T[CT][GA]AG 1217 CTCAAG SspBI 491 TGTACA T^GTACA 4322 TGTACA Tth111I 382 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1682 GACGGAGTC XcmI 2849 CCAAGCCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3558 CCACCCACATTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII