(Circular) (Six-base) MAPSORT of: pFN21AE2388.seq Check: 375 from: 1 to: 9157 pFN21AE2388 9157 bp With 182 enzymes: SgfI * February 7, 2012 16:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4669 4669 Size: 9157 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6967 278 Size: 53 83 186 936 5431 2468 Fragments arranged by size: 5431 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3155 3563 4983 6500 1937 Size: 1218 408 1420 1517 4594 Fragments arranged by size: 4594 1517 1420 1218 408 Acc65I G'GTAC_C Cuts at: 6478 6478 Size: 9157 AclI AA'CG_TT Cuts at: 1563 5321 7284 7657 1563 Size: 3758 1963 373 3063 Fragments arranged by size: 3758 3063 1963 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2180 4956 7220 8604 8872 1632 Size: 548 2776 2264 1384 268 1917 Fragments arranged by size: 2776 2264 1917 1384 548 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8960 AflIII A'CryG_T Cuts at: 1203 8056 9033 1203 Size: 6853 977 1327 Fragments arranged by size: 6853 1327 977 AgeI A'CCGG_T Cuts at: 7988 7988 Size: 9157 AhdI GACnn_n'nnGTC Cuts at: 2932 3162 4514 7886 2932 Size: 230 1352 3372 4203 Fragments arranged by size: 4203 3372 1352 230 AleI CACnn'nnGTG Cuts at: 1166 2342 1166 Size: 1176 7981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5339 1184 Size: 4155 5002 AlwNI CAG_nnn'CTG Cuts at: 2326 3995 4841 5861 6900 8472 2326 Size: 1669 846 1020 1039 1572 3011 Fragments arranged by size: 3011 1669 1572 1039 1020 846 ApaI G_GGCC'C Cuts at: 4175 4427 5072 5495 4175 Size: 252 645 423 7837 Fragments arranged by size: 7837 645 423 252 ApaLI G'TGCA_C Cuts at: 7214 8370 7214 Size: 1156 8001 ApoI r'AATT_y Cuts at: 1493 2671 3497 3650 4323 5446 5948 6466 6692 Size: 1178 826 153 673 1123 502 518 226 Cuts at: 6692 6844 8953 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1178 1123 826 673 518 502 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 7711 160 Size: 872 6679 1606 Fragments arranged by size: 6679 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9157 AvaI C'yCGr_G Cuts at: 1870 1943 4037 4497 6482 1870 Size: 73 2094 460 1985 4545 Fragments arranged by size: 4545 2094 1985 460 73 AvrII C'CTAG_G Cuts at: 2912 2912 Size: 9157 BamHI G'GATC_C Cuts at: 4184 6487 6854 4184 Size: 2303 367 6487 Fragments arranged by size: 6487 2303 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3928 4882 6415 6478 7933 Size: 325 217 627 2141 954 1533 63 1455 Cuts at: 7933 618 Size: 1842 Fragments arranged by size: 2141 1842 1533 1455 954 627 325 217 63 BanII G_rGCy'C Cuts at: 1391 2046 2280 3237 4175 4288 4427 5045 5072 Size: 655 234 957 938 113 139 618 27 Cuts at: 5072 5079 5495 5996 6476 1391 Size: 7 416 501 480 4072 Fragments arranged by size: 4072 957 938 655 618 501 480 416 234 139 113 27 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2386 2457 2617 3521 928 Size: 971 487 71 160 904 6564 Fragments arranged by size: 6564 971 904 487 160 71 BbvCI CC'TCA_GC Cuts at: 6117 6117 Size: 9157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2845 2879 3135 3169 4575 4609 7348 Size: 34 908 34 256 34 1406 34 2739 Cuts at: 7348 7382 1903 Size: 34 3678 Fragments arranged by size: 3678 2739 1406 908 256 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7049 8259 7049 Size: 1210 7947 BclI T'GATC_A Cuts at: 1548 1548 Size: 9157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5313 7768 136 Size: 107 122 71 631 605 3641 2455 1525 Fragments arranged by size: 3641 2455 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2850 9152 2850 Size: 6302 2855 BlpI GC'TnA_GC Cuts at: 6574 6574 Size: 9157 Bme1580I G_kGCm'C Cuts at: 1790 2012 2467 3872 4175 4427 4885 5072 5495 Size: 222 455 1405 303 252 458 187 423 Cuts at: 5495 7218 8374 1790 Size: 1723 1156 2573 Fragments arranged by size: 2573 1723 1405 1156 458 455 423 303 252 222 187 BmgBI CAC'GTC Cuts at: 1318 2161 3349 1318 Size: 843 1188 7126 Fragments arranged by size: 7126 1188 843 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4635 6888 7846 Size: 416 53 83 399 24 3218 2253 958 Cuts at: 7846 442 Size: 1753 Fragments arranged by size: 3218 2253 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9157 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2065 2812 2065 Size: 747 8410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3157 3225 3645 3718 4038 4065 4224 4272 Size: 1918 68 420 73 320 27 159 48 Cuts at: 4272 4611 5595 7817 1239 Size: 339 984 2222 2579 Fragments arranged by size: 2579 2222 1918 984 420 339 320 159 73 68 48 27 Bpu10I CC'TnA_GC Cuts at: 3194 6117 3194 Size: 2923 6234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2560 3207 7282 8147 8445 2560 Size: 647 4075 865 298 3272 Fragments arranged by size: 4075 3272 865 647 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4501 5181 5698 7820 882 Size: 617 3002 680 517 2122 2219 Fragments arranged by size: 3002 2219 2122 680 617 517 BsaAI yAC'GTr Cuts at: 493 1204 4486 6948 493 Size: 711 3282 2462 2702 Fragments arranged by size: 3282 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 9151 9151 Size: 9157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6964 7346 7981 275 Size: 53 83 186 936 5431 382 635 1451 Fragments arranged by size: 5431 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4885 7590 7988 8262 8409 8820 4885 Size: 2705 398 274 147 411 5222 Fragments arranged by size: 5222 2705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2633 2663 5385 5415 6088 Size: 30 165 30 1230 30 2722 30 673 Cuts at: 6088 6118 1178 Size: 30 4217 Fragments arranged by size: 4217 2722 1230 673 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2144 2654 2816 2972 3017 3104 3199 Size: 844 114 510 162 156 45 87 95 Cuts at: 3199 4003 4142 4729 5080 6018 6425 1186 Size: 804 139 587 351 938 407 3918 Fragments arranged by size: 3918 938 844 804 587 510 407 351 162 156 139 114 95 87 45 BseYI C'CCAG_C Cuts at: 2035 2271 3698 3920 4331 5164 5369 5515 5529 Size: 236 1427 222 411 833 205 146 14 Cuts at: 5529 5740 6198 8360 8856 2035 Size: 211 458 2162 496 2336 Fragments arranged by size: 2336 2162 1427 833 496 458 411 236 222 211 205 146 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 3056 3139 4070 4703 4770 5014 Size: 573 603 717 83 931 633 67 244 Cuts at: 5014 1163 Size: 5306 Fragments arranged by size: 5306 931 717 633 603 573 244 83 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 4349 4589 6125 6607 7368 Size: 275 186 30 2318 240 1536 482 761 Cuts at: 7368 8396 1540 Size: 1028 2301 Fragments arranged by size: 2318 2301 1536 1028 761 482 275 240 186 30 BsiHKAI G_wGCw'C Cuts at: 2046 2280 3237 4660 5045 5079 5996 7218 7303 Size: 234 957 1423 385 34 917 1222 85 Cuts at: 7303 8374 2046 Size: 1071 2829 Fragments arranged by size: 2829 1423 1222 1071 957 917 385 234 85 34 BsmI GAATG_Cn' Cuts at: 5791 5827 6348 6668 6761 5791 Size: 36 521 320 93 8187 Fragments arranged by size: 8187 521 320 93 36 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2046 2280 2467 3237 3872 4175 Size: 399 222 34 234 187 770 635 303 Cuts at: 4175 4288 4427 4660 4885 5045 5072 5079 5495 Size: 113 139 233 225 160 27 7 416 Cuts at: 5495 5996 6476 7218 7303 8374 1391 Size: 501 480 742 85 1071 2174 Fragments arranged by size: 2174 1071 770 742 635 501 480 416 399 303 234 233 225 222 187 160 139 113 85 34 27 7 BspEI T'CCGG_A Cuts at: 8820 8820 Size: 9157 BspHI T'CATG_A Cuts at: 4503 7046 8776 4503 Size: 2543 1730 4884 Fragments arranged by size: 4884 2543 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 4669 4807 6512 844 Size: 1490 2335 138 1705 3489 Fragments arranged by size: 3489 2335 1705 1490 138 BsrBI CCG'CTC Cuts at: 1394 1823 2049 2144 3732 7044 1394 Size: 429 226 95 1588 3312 3507 Fragments arranged by size: 3507 3312 1588 429 226 95 BsrDI GCAATG_nn' Cuts at: 65 1248 3612 5124 7652 7826 65 Size: 1183 2364 1512 2528 174 1396 Fragments arranged by size: 2528 2364 1512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2373 6124 7801 7988 1700 Size: 110 563 3751 1677 187 2869 Fragments arranged by size: 3751 2869 1677 563 187 110 BsrGI T'GTAC_A Cuts at: 96 2496 6327 96 Size: 2400 3831 2926 Fragments arranged by size: 3831 2926 2400 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9157 BssSI C'ACGA_G Cuts at: 7217 8229 7217 Size: 1012 8145 BstAPI GCAn_nnn'nTGC Cuts at: 794 5861 794 Size: 5067 4090 BstBI TT'CG_AA Cuts at: 7961 7961 Size: 9157 BstEII G'GTnAC_C Cuts at: 1184 2770 1184 Size: 1586 7571 BstYI r'GATC_y Cuts at: 1972 2349 2850 3991 4184 6487 6854 7239 7256 Size: 377 501 1141 193 2303 367 385 17 Cuts at: 7256 8708 8794 8806 9152 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2303 1977 1452 1141 501 385 377 367 346 193 86 17 12 BstZ17I GTA'TAC Cuts at: 3564 3564 Size: 9157 Bsu36I CC'TnA_GG Cuts at: 4573 4573 Size: 9157 BtgI C'CryG_G Cuts at: 513 1065 3438 3771 3857 4396 5196 5790 513 Size: 552 2373 333 86 539 800 594 3880 Fragments arranged by size: 3880 2373 800 594 552 539 333 86 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2056 4739 487 Size: 683 56 830 2683 4905 Fragments arranged by size: 4905 2683 830 683 56 BtsI GCAGTG_nn' Cuts at: 3039 3111 3127 3324 5290 5862 6673 7467 7487 Size: 72 16 197 1966 572 811 794 20 Cuts at: 7487 3039 Size: 4709 Fragments arranged by size: 4709 1966 811 794 572 197 72 20 16 ClaI AT'CG_AT Cuts at: 1554 4345 1554 Size: 2791 6366 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9122 DraI TTT'AAA Cuts at: 3939 6461 3939 Size: 2522 6635 DraIII CAC_nnn'GTG Cuts at: 4961 6085 4961 Size: 1124 8033 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8164 809 Size: 507 6848 1802 Fragments arranged by size: 6848 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2067 3880 Size: 54 1425 50 275 18 198 39 1813 Cuts at: 3880 4295 5793 6122 6126 6604 7493 9122 8 Size: 415 1498 329 4 478 889 1629 43 Fragments arranged by size: 1813 1629 1498 1425 889 478 415 329 275 198 54 50 43 39 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2028 6122 6604 1537 Size: 275 216 4094 482 4090 Fragments arranged by size: 4094 4090 482 275 216 EarI CTCTTCn'nnn_ Cuts at: 1353 2151 2209 2337 2599 2748 3006 3063 3176 Size: 798 58 128 262 149 258 57 113 Cuts at: 3176 3823 4677 4941 5777 5898 6160 7087 1353 Size: 647 854 264 836 121 262 927 3423 Fragments arranged by size: 3423 927 854 836 798 647 264 262 262 258 149 128 121 113 58 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2096 6021 7744 8118 8264 387 Size: 1064 138 507 3925 1723 374 146 1280 Fragments arranged by size: 3925 1723 1280 1064 507 374 146 138 EcoICRI GAG'CTC Cuts at: 2044 2278 3235 5043 5077 5994 2044 Size: 234 957 1808 34 917 5207 Fragments arranged by size: 5207 1808 957 917 234 34 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 3157 3225 3645 3718 4038 4065 Size: 393 548 977 68 420 73 320 27 Cuts at: 4065 4224 4272 4611 4956 5595 7220 7817 8604 Size: 159 48 339 345 639 1625 597 787 Cuts at: 8604 8872 1239 Size: 268 1524 Fragments arranged by size: 1625 1524 977 787 639 597 548 420 393 345 339 320 268 159 73 68 48 27 EcoNI CCTnn'n_nnAGG Cuts at: 2195 2831 3981 4572 6215 2195 Size: 636 1150 591 1643 5137 Fragments arranged by size: 5137 1643 1150 636 591 EcoO109I rG'GnC_Cy Cuts at: 2062 2323 2410 2506 2706 2793 3433 3739 4106 Size: 261 87 96 200 87 640 306 367 Cuts at: 4106 4415 4454 5272 5492 5766 6101 6224 6311 Size: 309 39 818 220 274 335 123 87 Cuts at: 6311 2062 Size: 4908 Fragments arranged by size: 4908 818 640 367 335 309 306 274 261 220 200 123 96 87 87 87 39 EcoRI G'AATT_C Cuts at: 5446 6466 6844 5446 Size: 1020 378 7759 Fragments arranged by size: 7759 1020 378 EcoRV GAT'ATC Cuts at: 2221 2221 Size: 9157 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2162 6330 2162 Size: 4168 4989 FseI GG_CCGG'CC Cuts at: 6128 6128 Size: 9157 FspI TGC'GCA Cuts at: 7663 7663 Size: 9157 HaeII r_GCGC'y Cuts at: 1678 3815 5217 8304 1678 Size: 2137 1402 3087 2531 Fragments arranged by size: 3087 2531 2137 1402 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2918 2950 3148 3180 4500 Size: 32 135 32 1346 32 198 32 1320 Cuts at: 4500 4532 6088 6120 7798 7830 7872 7904 1373 Size: 32 1556 32 1678 32 42 32 2626 Fragments arranged by size: 2626 1678 1556 1346 1320 198 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3156 6501 6747 669 Size: 988 281 1218 3345 246 3079 Fragments arranged by size: 3345 3079 1218 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9157 HpaI GTT'AAC Cuts at: 6747 6747 Size: 9157 KpnI G_GTAC'C Cuts at: 6482 6482 Size: 9157 MfeI C'AATT_G Cuts at: 6756 6756 Size: 9157 MluI A'CGCG_T Cuts at: 9033 9033 Size: 9157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3066 3291 4066 5864 6458 8271 8455 1305 Size: 1761 225 775 1798 594 1813 184 2007 Fragments arranged by size: 2007 1813 1798 1761 775 594 225 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5795 10 Size: 54 1425 343 3963 3372 Fragments arranged by size: 3963 3372 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2154 2342 3329 3909 7115 7474 7633 Size: 648 988 188 987 580 3206 359 159 Cuts at: 7633 518 Size: 2042 Fragments arranged by size: 3206 2042 988 987 648 580 359 188 159 MspA1I CmG'CkG Cuts at: 1606 2271 3859 5419 5453 6121 6573 7250 8398 Size: 665 1588 1560 34 668 452 677 1148 Cuts at: 8398 8643 1606 Size: 245 2120 Fragments arranged by size: 2120 1588 1560 1148 677 668 665 452 245 34 NaeI GCC'GGC Cuts at: 6126 6126 Size: 9157 NcoI C'CATG_G Cuts at: 513 1065 3438 3771 4396 5790 513 Size: 552 2373 333 625 1394 3880 Fragments arranged by size: 3880 2373 1394 625 552 333 NdeI CA'TA_TG Cuts at: 387 4338 387 Size: 3951 5206 NgoMIV G'CCGG_C Cuts at: 6124 6124 Size: 9157 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9157 NotI GC'GGCC_GC Cuts at: 6604 6604 Size: 9157 NruI TCG'CGA Cuts at: 2787 2787 Size: 9157 NspI r_CATG'y Cuts at: 1132 1229 3150 4234 4585 6515 8060 1132 Size: 97 1921 1084 351 1930 1545 2229 Fragments arranged by size: 2229 1930 1921 1545 1084 351 97 PasI CC'CwG_GG Cuts at: 1348 4864 4874 5719 6144 6188 1348 Size: 3516 10 845 425 44 4317 Fragments arranged by size: 4317 3516 845 425 44 10 PciI A'CATG_T Cuts at: 8056 8056 Size: 9157 PflMI CCAn_nnn'nTGG Cuts at: 1108 4181 4530 4964 5573 1108 Size: 3073 349 434 609 4692 Fragments arranged by size: 4692 3073 609 434 349 PfoI T'CCnGG_A Cuts at: 2319 2584 3622 4023 6318 2319 Size: 265 1038 401 2295 5158 Fragments arranged by size: 5158 2295 1038 401 265 PmeI GTTT'AAAC Cuts at: 6461 6461 Size: 9157 PmlI CAC'GTG Cuts at: 4486 4486 Size: 9157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2799 2831 7226 7258 8739 8771 2799 Size: 32 4395 32 1481 32 3185 Fragments arranged by size: 4395 3185 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2323 2506 2706 2793 3739 4106 4415 4454 Size: 261 183 200 87 946 367 309 39 Cuts at: 4454 5766 6224 6311 2062 Size: 1312 458 87 4908 Fragments arranged by size: 4908 1312 946 458 367 309 261 200 183 87 87 39 PshAI GACnn'nnGTC Cuts at: 1613 2704 1613 Size: 1091 8066 PsiI TTA'TAA Cuts at: 6727 6727 Size: 9157 PspOMI G'GGCC_C Cuts at: 4171 4423 5068 5491 4171 Size: 252 645 423 7837 Fragments arranged by size: 7837 645 423 252 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5272 5304 5272 Size: 32 9125 PstI C_TGCA'G Cuts at: 830 2637 5022 5484 6096 6509 830 Size: 1807 2385 462 612 413 3478 Fragments arranged by size: 3478 2385 1807 612 462 413 PvuI CG_AT'CG Cuts at: 2001 4349 2001 Size: 2348 6809 PvuII CAG'CTG Cuts at: 5453 5453 Size: 9157 SacI G_AGCT'C Cuts at: 2046 2280 3237 5045 5079 5996 2046 Size: 234 957 1808 34 917 5207 Fragments arranged by size: 5207 1808 957 917 234 34 SacII CC_GC'GG Cuts at: 3860 3860 Size: 9157 SalI G'TCGA_C Cuts at: 1936 3154 6499 1936 Size: 1218 3345 4594 Fragments arranged by size: 4594 3345 1218 SanDI GG'GwC_CC Cuts at: 2706 3739 6224 2706 Size: 1033 2485 5639 Fragments arranged by size: 5639 2485 1033 SapI GCTCTTCn'nnn_ Cuts at: 2151 3006 4677 2151 Size: 855 1671 6631 Fragments arranged by size: 6631 1671 855 SbfI CC_TGCA'GG Cuts at: 5484 6509 5484 Size: 1025 8132 ScaI AGT'ACT Cuts at: 7405 9117 7405 Size: 1712 7445 SexAI A'CCwGG_T Cuts at: 4957 4957 Size: 9157 SfcI C'TryA_G Cuts at: 826 1045 2633 3168 5018 5480 6092 6505 7640 Size: 219 1588 535 1850 462 612 413 1135 Cuts at: 7640 8321 8512 826 Size: 681 191 1471 Fragments arranged by size: 1850 1588 1471 1135 681 612 535 462 413 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9157 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9157 SmaI CCC'GGG Cuts at: 1872 4039 6484 1872 Size: 2167 2445 4545 Fragments arranged by size: 4545 2445 2167 SmlI C'TyrA_G Cuts at: 820 1017 1943 2575 3222 7261 8162 8424 820 Size: 197 926 632 647 4039 901 262 1553 Fragments arranged by size: 4039 1553 926 901 647 632 262 197 SnaBI TAC'GTA Cuts at: 493 6948 493 Size: 6455 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9157 SphI G_CATG'C Cuts at: 1132 6515 1132 Size: 5383 3774 SspI AAT'ATT Cuts at: 5 52 7081 5 Size: 47 7029 2081 Fragments arranged by size: 7029 2081 47 StuI AGG'CCT Cuts at: 1517 5478 6252 6371 1517 Size: 3961 774 119 4303 Fragments arranged by size: 4303 3961 774 119 StyI C'CwwG_G Cuts at: 513 1065 2676 2710 2722 2912 3438 3489 3771 Size: 552 1611 34 12 190 526 51 282 Cuts at: 3771 4396 4411 4419 5173 5275 5543 5762 5790 Size: 625 15 8 754 102 268 219 28 Cuts at: 5790 6596 513 Size: 806 3074 Fragments arranged by size: 3074 1611 806 754 625 552 526 282 268 219 190 102 51 34 28 15 12 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2435 3934 4469 4654 4842 5580 5640 Size: 1255 855 1499 535 185 188 738 60 Cuts at: 5640 6201 6212 7184 7201 7354 7539 7984 325 Size: 561 11 972 17 153 185 445 1498 Fragments arranged by size: 1499 1498 1255 972 855 738 561 535 445 188 185 185 153 60 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 4369 6327 Size: 274 80 33 51 1443 519 1873 1958 Cuts at: 6327 7403 9115 96 Size: 1076 1712 138 Fragments arranged by size: 1958 1873 1712 1443 1076 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3101 3133 3353 3385 4169 4201 4384 4416 5385 Size: 32 220 32 784 32 183 32 969 Cuts at: 5385 5417 9117 9149 3101 Size: 32 3700 32 3109 Fragments arranged by size: 3700 3109 969 784 220 183 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2390 3690 1316 Size: 1074 1300 6783 Fragments arranged by size: 6783 1300 1074 XbaI T'CTAG_A Cuts at: 3343 6493 3343 Size: 3150 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4861 5570 4861 Size: 709 8448 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9157 XmaI C'CCGG_G Cuts at: 1870 4037 6482 1870 Size: 2167 2445 4545 Fragments arranged by size: 4545 2445 2167 XmnI GAAnn'nnTTC Cuts at: 7286 7286 Size: 9157 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6965 276 Size: 53 83 186 936 5431 2468 Fragments arranged by size: 5431 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI FspAI KasI NarI NsiI PacI PspXI RsrII SfiI SfoI SrfI SwaI