(Linear) (Six-base) MAPSORT of: KIEE2388.seq Check: 9123 from: 1 to: 4452 KIEE2388.orf With 182 enzymes: SgfI * February 7, 2012 15:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2664 4452 Size: 2664 1788 AccI GT'mk_AC Cuts at: 0 1150 1558 2978 4452 Size: 1150 408 1420 1474 Fragments arranged by size: 1474 1420 1150 408 AclI AA'CG_TT Cuts at: 0 3316 4452 Size: 3316 1136 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 2951 4452 Size: 175 2776 1501 Fragments arranged by size: 2776 1501 175 AhdI GACnn_n'nnGTC Cuts at: 0 927 1157 2509 4452 Size: 927 230 1352 1943 Fragments arranged by size: 1943 1352 927 230 AleI CACnn'nnGTG Cuts at: 0 337 4452 Size: 337 4115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3334 4452 Size: 3334 1118 AlwNI CAG_nnn'CTG Cuts at: 0 321 1990 2836 3856 4452 Size: 321 1669 846 1020 596 Fragments arranged by size: 1669 1020 846 596 321 ApaI G_GGCC'C Cuts at: 0 2170 2422 3067 3490 4452 Size: 2170 252 645 423 962 Fragments arranged by size: 2170 962 645 423 252 ApoI r'AATT_y Cuts at: 0 666 1492 1645 2318 3441 3943 4452 Size: 666 826 153 673 1123 502 509 Fragments arranged by size: 1123 826 673 666 509 502 153 AvaI C'yCGr_G Cuts at: 0 2032 2492 4452 Size: 2032 460 1960 Fragments arranged by size: 2032 1960 460 AvrII C'CTAG_G Cuts at: 0 907 4452 Size: 907 3545 BamHI G'GATC_C Cuts at: 0 2179 4452 Size: 2179 2273 BanI G'GyrC_C Cuts at: 0 1923 2877 4410 4452 Size: 1923 954 1533 42 Fragments arranged by size: 1923 1533 954 42 BanII G_rGCy'C Cuts at: 0 41 275 1232 2170 2283 2422 3040 3067 Size: 41 234 957 938 113 139 618 27 Cuts at: 3067 3074 3490 3991 4452 Size: 7 416 501 461 Fragments arranged by size: 957 938 618 501 461 416 234 139 113 41 27 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 381 452 612 1516 4452 Size: 381 71 160 904 2936 Fragments arranged by size: 2936 904 381 160 71 BbvCI CC'TCA_GC Cuts at: 0 4112 4452 Size: 4112 340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 840 874 1130 1164 2570 2604 4452 Size: 840 34 256 34 1406 34 1848 Fragments arranged by size: 1848 1406 840 256 34 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 3308 4452 Size: 3308 1144 BglII A'GATC_T Cuts at: 0 845 4452 Size: 845 3607 Bme1580I G_kGCm'C Cuts at: 0 7 462 1867 2170 2422 2880 3067 3490 Size: 7 455 1405 303 252 458 187 423 Cuts at: 3490 4452 Size: 962 Fragments arranged by size: 1405 962 458 455 423 303 252 187 7 BmgBI CAC'GTC Cuts at: 0 156 1344 4452 Size: 156 1188 3108 Fragments arranged by size: 3108 1188 156 BmrI ACTGGGnnnn_n' Cuts at: 0 2630 4452 Size: 2630 1822 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 60 807 4452 Size: 60 747 3645 Fragments arranged by size: 3645 747 60 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1152 1220 1640 1713 2033 2060 2219 2267 Size: 1152 68 420 73 320 27 159 48 Cuts at: 2267 2606 3590 4452 Size: 339 984 862 Fragments arranged by size: 1152 984 862 420 339 320 159 73 68 48 27 Bpu10I CC'TnA_GC Cuts at: 0 1189 4112 4452 Size: 1189 2923 340 Fragments arranged by size: 2923 1189 340 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 555 1202 4452 Size: 555 647 3250 Fragments arranged by size: 3250 647 555 BsaI GGTCTCn'nnnn_ Cuts at: 0 2496 3176 3693 4452 Size: 2496 680 517 759 Fragments arranged by size: 2496 759 680 517 BsaAI yAC'GTr Cuts at: 0 2481 4452 Size: 2481 1971 BsaWI w'CCGG_w Cuts at: 0 2880 4452 Size: 2880 1572 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 628 658 3380 3410 4083 4113 4452 Size: 628 30 2722 30 673 30 339 Fragments arranged by size: 2722 673 628 339 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 139 649 811 967 1012 1099 1194 Size: 25 114 510 162 156 45 87 95 Cuts at: 1194 1998 2137 2724 3075 4013 4420 4452 Size: 804 139 587 351 938 407 32 Fragments arranged by size: 938 804 587 510 407 351 162 156 139 114 95 87 45 32 25 BseYI C'CCAG_C Cuts at: 0 30 266 1693 1915 2326 3159 3364 3510 Size: 30 236 1427 222 411 833 205 146 Cuts at: 3510 3524 3735 4193 4452 Size: 14 211 458 259 Fragments arranged by size: 1427 833 458 411 259 236 222 211 205 146 30 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 1051 1134 2065 2698 2765 3009 4452 Size: 334 717 83 931 633 67 244 1443 Fragments arranged by size: 1443 931 717 633 334 244 83 67 BsiEI CG_ry'CG Cuts at: 0 26 2344 2584 4120 4452 Size: 26 2318 240 1536 332 Fragments arranged by size: 2318 1536 332 240 26 BsiHKAI G_wGCw'C Cuts at: 0 41 275 1232 2655 3040 3074 3991 4452 Size: 41 234 957 1423 385 34 917 461 Fragments arranged by size: 1423 957 917 461 385 234 41 34 BsmI GAATG_Cn' Cuts at: 0 3786 3822 4343 4452 Size: 3786 36 521 109 Fragments arranged by size: 3786 521 109 36 Bsp1286I G_dGCh'C Cuts at: 0 7 41 275 462 1232 1867 2170 2283 Size: 7 34 234 187 770 635 303 113 Cuts at: 2283 2422 2655 2880 3040 3067 3074 3490 3991 Size: 139 233 225 160 27 7 416 501 Cuts at: 3991 4452 Size: 461 Fragments arranged by size: 770 635 501 461 416 303 234 233 225 187 160 139 113 34 27 7 7 BspHI T'CATG_A Cuts at: 0 2498 4452 Size: 2498 1954 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 329 2664 2802 4452 Size: 329 2335 138 1650 Fragments arranged by size: 2335 1650 329 138 BsrBI CCG'CTC Cuts at: 0 44 139 1727 4452 Size: 44 95 1588 2725 Fragments arranged by size: 2725 1588 95 44 BsrDI GCAATG_nn' Cuts at: 0 1607 3119 4452 Size: 1607 1512 1333 Fragments arranged by size: 1607 1512 1333 BsrFI r'CCGG_y Cuts at: 0 368 4119 4452 Size: 368 3751 333 Fragments arranged by size: 3751 368 333 BsrGI T'GTAC_A Cuts at: 0 491 4322 4452 Size: 491 3831 130 Fragments arranged by size: 3831 491 130 BstAPI GCAn_nnn'nTGC Cuts at: 0 3856 4452 Size: 3856 596 BstEII G'GTnAC_C Cuts at: 0 765 4452 Size: 765 3687 BstYI r'GATC_y Cuts at: 0 344 845 1986 2179 4452 Size: 344 501 1141 193 2273 Fragments arranged by size: 2273 1141 501 344 193 BstZ17I GTA'TAC Cuts at: 0 1559 4452 Size: 1559 2893 Bsu36I CC'TnA_GG Cuts at: 0 2568 4452 Size: 2568 1884 BtgI C'CryG_G Cuts at: 0 1433 1766 1852 2391 3191 3785 4452 Size: 1433 333 86 539 800 594 667 Fragments arranged by size: 1433 800 667 594 539 333 86 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 51 2734 4452 Size: 51 2683 1718 Fragments arranged by size: 2683 1718 51 BtsI GCAGTG_nn' Cuts at: 0 1034 1106 1122 1319 3285 3857 4452 Size: 1034 72 16 197 1966 572 595 Fragments arranged by size: 1966 1034 595 572 197 72 16 ClaI AT'CG_AT Cuts at: 0 2340 4452 Size: 2340 2112 DraI TTT'AAA Cuts at: 0 1934 4452 Size: 1934 2518 DraIII CAC_nnn'GTG Cuts at: 0 2956 4080 4452 Size: 2956 1124 372 Fragments arranged by size: 2956 1124 372 EaeI y'GGCC_r Cuts at: 0 23 62 1875 2290 3788 4117 4121 4452 Size: 23 39 1813 415 1498 329 4 331 Fragments arranged by size: 1813 1498 415 331 329 39 23 4 EagI C'GGCC_G Cuts at: 0 23 4117 4452 Size: 23 4094 335 Fragments arranged by size: 4094 335 23 EarI CTCTTCn'nnn_ Cuts at: 0 146 204 332 594 743 1001 1058 1171 Size: 146 58 128 262 149 258 57 113 Cuts at: 1171 1818 2672 2936 3772 3893 4155 4452 Size: 647 854 264 836 121 262 297 Fragments arranged by size: 854 836 647 297 264 262 262 258 149 146 128 121 113 58 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 91 4016 4452 Size: 91 3925 436 Fragments arranged by size: 3925 436 91 EcoICRI GAG'CTC Cuts at: 0 39 273 1230 3038 3072 3989 4452 Size: 39 234 957 1808 34 917 463 Fragments arranged by size: 1808 957 917 463 234 39 34 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 1152 1220 1640 1713 2033 2060 2219 Size: 175 977 68 420 73 320 27 159 Cuts at: 2219 2267 2606 2951 3590 4452 Size: 48 339 345 639 862 Fragments arranged by size: 977 862 639 420 345 339 320 175 159 73 68 48 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 190 826 1976 2567 4210 4452 Size: 190 636 1150 591 1643 242 Fragments arranged by size: 1643 1150 636 591 242 190 EcoO109I rG'GnC_Cy Cuts at: 0 57 318 405 501 701 788 1428 1734 Size: 57 261 87 96 200 87 640 306 Cuts at: 1734 2101 2410 2449 3267 3487 3761 4096 4219 Size: 367 309 39 818 220 274 335 123 Cuts at: 4219 4306 4452 Size: 87 146 Fragments arranged by size: 818 640 367 335 309 306 274 261 220 200 146 123 96 87 87 87 57 39 EcoRI G'AATT_C Cuts at: 0 3441 4452 Size: 3441 1011 EcoRV GAT'ATC Cuts at: 0 216 4452 Size: 216 4236 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 157 4325 4452 Size: 157 4168 127 Fragments arranged by size: 4168 157 127 FseI GG_CCGG'CC Cuts at: 0 4123 4452 Size: 4123 329 HaeII r_GCGC'y Cuts at: 0 1810 3212 4452 Size: 1810 1402 1240 Fragments arranged by size: 1810 1402 1240 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 913 945 1143 1175 2495 2527 4083 4115 Size: 913 32 198 32 1320 32 1556 32 Cuts at: 4115 4452 Size: 337 Fragments arranged by size: 1556 1320 913 337 198 32 32 32 32 HincII GTy'rAC Cuts at: 0 1151 4452 Size: 1151 3301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1061 1286 2061 3859 4452 Size: 1061 225 775 1798 593 Fragments arranged by size: 1798 1061 775 593 225 MscI TGG'CCA Cuts at: 0 3790 4452 Size: 3790 662 MslI CAynn'nnrTG Cuts at: 0 149 337 1324 1904 4452 Size: 149 188 987 580 2548 Fragments arranged by size: 2548 987 580 188 149 MspA1I CmG'CkG Cuts at: 0 266 1854 3414 3448 4116 4452 Size: 266 1588 1560 34 668 336 Fragments arranged by size: 1588 1560 668 336 266 34 NaeI GCC'GGC Cuts at: 0 4121 4452 Size: 4121 331 NcoI C'CATG_G Cuts at: 0 1433 1766 2391 3785 4452 Size: 1433 333 625 1394 667 Fragments arranged by size: 1433 1394 667 625 333 NdeI CA'TA_TG Cuts at: 0 2333 4452 Size: 2333 2119 NgoMIV G'CCGG_C Cuts at: 0 4119 4452 Size: 4119 333 NruI TCG'CGA Cuts at: 0 782 4452 Size: 782 3670 NspI r_CATG'y Cuts at: 0 1145 2229 2580 4452 Size: 1145 1084 351 1872 Fragments arranged by size: 1872 1145 1084 351 PasI CC'CwG_GG Cuts at: 0 2859 2869 3714 4139 4183 4452 Size: 2859 10 845 425 44 269 Fragments arranged by size: 2859 845 425 269 44 10 PflMI CCAn_nnn'nTGG Cuts at: 0 2176 2525 2959 3568 4452 Size: 2176 349 434 609 884 Fragments arranged by size: 2176 884 609 434 349 PfoI T'CCnGG_A Cuts at: 0 314 579 1617 2018 4313 4452 Size: 314 265 1038 401 2295 139 Fragments arranged by size: 2295 1038 401 314 265 139 PmlI CAC'GTG Cuts at: 0 2481 4452 Size: 2481 1971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 794 826 4452 Size: 794 32 3626 Fragments arranged by size: 3626 794 32 PpuMI rG'GwC_Cy Cuts at: 0 57 318 501 701 788 1734 2101 2410 Size: 57 261 183 200 87 946 367 309 Cuts at: 2410 2449 3761 4219 4306 4452 Size: 39 1312 458 87 146 Fragments arranged by size: 1312 946 458 367 309 261 200 183 146 87 87 57 39 PshAI GACnn'nnGTC Cuts at: 0 699 4452 Size: 699 3753 PspOMI G'GGCC_C Cuts at: 0 2166 2418 3063 3486 4452 Size: 2166 252 645 423 966 Fragments arranged by size: 2166 966 645 423 252 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3267 3299 4452 Size: 3267 32 1153 Fragments arranged by size: 3267 1153 32 PstI C_TGCA'G Cuts at: 0 632 3017 3479 4091 4452 Size: 632 2385 462 612 361 Fragments arranged by size: 2385 632 612 462 361 PvuI CG_AT'CG Cuts at: 0 2344 4452 Size: 2344 2108 PvuII CAG'CTG Cuts at: 0 3448 4452 Size: 3448 1004 SacI G_AGCT'C Cuts at: 0 41 275 1232 3040 3074 3991 4452 Size: 41 234 957 1808 34 917 461 Fragments arranged by size: 1808 957 917 461 234 41 34 SacII CC_GC'GG Cuts at: 0 1855 4452 Size: 1855 2597 SalI G'TCGA_C Cuts at: 0 1149 4452 Size: 1149 3303 SanDI GG'GwC_CC Cuts at: 0 701 1734 4219 4452 Size: 701 1033 2485 233 Fragments arranged by size: 2485 1033 701 233 SapI GCTCTTCn'nnn_ Cuts at: 0 146 1001 2672 4452 Size: 146 855 1671 1780 Fragments arranged by size: 1780 1671 855 146 SbfI CC_TGCA'GG Cuts at: 0 3479 4452 Size: 3479 973 SexAI A'CCwGG_T Cuts at: 0 2952 4452 Size: 2952 1500 SfcI C'TryA_G Cuts at: 0 628 1163 3013 3475 4087 4452 Size: 628 535 1850 462 612 365 Fragments arranged by size: 1850 628 612 535 462 365 SmaI CCC'GGG Cuts at: 0 2034 4452 Size: 2034 2418 SmlI C'TyrA_G Cuts at: 0 570 1217 4452 Size: 570 647 3235 Fragments arranged by size: 3235 647 570 StuI AGG'CCT Cuts at: 0 3473 4247 4366 4452 Size: 3473 774 119 86 Fragments arranged by size: 3473 774 119 86 StyI C'CwwG_G Cuts at: 0 671 705 717 907 1433 1484 1766 2391 Size: 671 34 12 190 526 51 282 625 Cuts at: 2391 2406 2414 3168 3270 3538 3757 3785 4452 Size: 15 8 754 102 268 219 28 667 Fragments arranged by size: 754 671 667 625 526 282 268 219 190 102 51 34 28 15 12 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 430 1929 2464 2649 2837 3575 3635 4196 Size: 430 1499 535 185 188 738 60 561 Cuts at: 4196 4207 4452 Size: 11 245 Fragments arranged by size: 1499 738 561 535 430 245 188 185 60 11 TatI w'GTAC_w Cuts at: 0 491 2364 4322 4452 Size: 491 1873 1958 130 Fragments arranged by size: 1958 1873 491 130 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1096 1128 1348 1380 2164 2196 2379 2411 Size: 1096 32 220 32 784 32 183 32 Cuts at: 2411 3380 3412 4452 Size: 969 32 1040 Fragments arranged by size: 1096 1040 969 784 220 183 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 385 1685 4452 Size: 385 1300 2767 Fragments arranged by size: 2767 1300 385 XbaI T'CTAG_A Cuts at: 0 1338 4452 Size: 1338 3114 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2856 3565 4452 Size: 2856 709 887 Fragments arranged by size: 2856 887 709 XmaI C'CCGG_G Cuts at: 0 2032 4452 Size: 2032 2420 Enzymes that do cut: AarI AccI AclI AcuI AhdI AleI AlfI AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NruI NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII Acc65I AfeI AflII AflIII AgeI AloI ApaLI AscI AseI AsiSI BaeI BbeI BciVI BclI BfrBI BlpI BmtI BsaBI BsaHI BsiWI BsmBI BspEI BssHII BssSI BstBI BstXI CspCI DrdI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NheI NotI NsiI PacI PciI PmeI PsiI PspXI RsrII ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XhoI XmnI ZraI