################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssHII BstBI BstEII BstPI BstXI Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI HincII HindII HpaI KpnI Ksp632I KspI MaeI MboII MfeI MroI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 918 GTATAC GT^[AC][GT]AC AflIII 1046 ACGCGT A^C[GA][CT]GT AvaI 947 CCCGAG C^[CT]CG[GA]G AvaII 249 GGTCC G^G[AT]CC AviII 506 TGCGCA TGC^GCA BbrPI 889 CACGTG CAC^GTG BlpI 1227 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1227 GCTTAGC GC^T[ACGT]AGC BsaAI 889 CACGTG [CT]AC^GT[GA] BsaWI 1073 ACCGGA [AT]^CCGG[AT] BseRI 214 CTCCTC GAGGAG(10/8) BsoBI 947 CCCGAG C^[CT]CG[GA]G BspMI 1139 ACCTGC ACCTGC(4/8) BsrFI 76 GCCGGC [GA]^CCGG[CT] BssSI 156 CTCGTG CACGAG(-5/-1) Bst1107I 918 GTATAC GTA^TAC BstAPI 318 GCAGATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 9 GGATCT [GA]^GATC[CT] BstZ17I 918 GTATAC GTA^TAC CelII 1227 GCTTAGC GC^T[ACGT]AGC Cfr10I 76 GCCGGC [GA]^CCGG[CT] DraIII 777 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 910 GATATC GAT^ATC FspI 506 TGCGCA TGC^GCA HindIII 589 AAGCTT A^AGCTT HphI 760 GGTGA GGTGA(8/7) MaeIII 761 GTGAC ^GT[ACGT]AC MflI 9 GGATCT [GA]^GATC[CT] MluI 1046 ACGCGT A^CGCGT NaeI 76 GCCGGC GCC^GGC NcoI 1100 CCATGG C^CATGG NgoMIV 76 GCCGGC G^CCGGC NspI 126 GCATGC [GA]CATG^[CT] PflMI 653 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 889 CACGTG CAC^GTG PmlI 889 CACGTG CAC^GTG PstI 1141 CTGCAG CTGCA^G PvuII 253 CAGCTG CAG^CTG SexAI 482 ACCTGGT A^CC[AT]GGT SfcI 1141 CTGCAG C^T[GA][CT]AG SfiI 846 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 33 CTTGAG C^T[CT][GA]AG SphI 126 GCATGC GCATG^C Tsp45I 761 GTGAC ^GT[GC]AC Van91I 653 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 9 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Alw44I 804 GTGCAC G^TGCAC 892 GTGCAC AlwNI 98 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 1147 CAGAATCTG Aor51HI 47 AGCGCT AGC^GCT 447 AGCGCT ApaI 369 GGGCCC GGGCC^C 1169 GGGCCC ApaLI 804 GTGCAC G^TGCAC 892 GTGCAC BalI 461 TGGCCA TGG^CCA 584 TGGCCA BanII 369 GGGCCC G[GA]GC[CT]^C 1169 GGGCCC BbeI 357 GGCGCC GGCGC^C 396 GGCGCC BglI 76 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 847 GCCGTGGTGGC BsaBI 729 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 906 GATGGATATC BsmBI 475 CGTCTC CGTCTC(1/5) 36 GAGACG BsmFI 281 GGGAC GGGAC(10/14) 456 GTCCC BspHI 260 TCATGA T^CATGA 902 TCATGA BstF5I 968 GGATG GGATG(2/0) 1209 GGATG Eco47III 47 AGCGCT AGC^GCT 447 AGCGCT EcoT14I 438 CCAAGG C^C[AT][AT]GG 1100 CCATGG FokI 968 GGATG GGATG(9/13) 1209 GGATG KasI 357 GGCGCC G^GCGCC 396 GGCGCC MaeII 764 ACGT A^CGT 890 ACGT MamI 729 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 906 GATGGATATC MluNI 461 TGGCCA TGG^CCA 584 TGGCCA MscI 461 TGGCCA TGG^CCA 584 TGGCCA NarI 357 GGCGCC GG^CGCC 396 GGCGCC RcaI 260 TCATGA T^CATGA 902 TCATGA SfoI 357 GGCGCC GGC^GCC 396 GGCGCC StyI 438 CCAAGG C^C[AT][AT]GG 1100 CCATGG TfiI 428 GATTC G^A[AT]TC 1149 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI BanI BbvI BcnI BmyI BpmI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MboI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI TspRI