(Circular) (Six-base) MAPSORT of: pFN21AE4414.seq Check: 2443 from: 1 to: 5986 pFN21AE4414 5986 bp With 182 enzymes: SgfI * February 2, 2012 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2924 3329 1937 Size: 987 405 4594 Fragments arranged by size: 4594 987 405 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4049 5433 5701 1632 Size: 2417 1384 268 1917 Fragments arranged by size: 2417 1917 1384 268 AfeI AGC'GCT Cuts at: 2054 2454 2054 Size: 400 5586 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 3051 4885 5862 1203 Size: 1848 1834 977 1327 Fragments arranged by size: 1848 1834 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2858 1184 Size: 1674 4312 AlwNI CAG_nnn'CTG Cuts at: 2108 3157 3729 5301 2108 Size: 1049 572 1572 2793 Fragments arranged by size: 2793 1572 1049 572 ApaI G_GGCC'C Cuts at: 2378 3178 2378 Size: 800 5186 ApaLI G'TGCA_C Cuts at: 2809 2897 4043 5199 2809 Size: 88 1146 1156 3596 Fragments arranged by size: 3596 1156 1146 88 ApoI r'AATT_y Cuts at: 1493 3295 3521 3673 5782 1493 Size: 1802 226 152 2109 1697 Fragments arranged by size: 2109 1802 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2952 3311 1870 Size: 73 1009 359 4545 Fragments arranged by size: 4545 1009 359 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2362 2401 2491 2709 2773 Size: 325 217 627 575 39 90 218 64 Cuts at: 2773 2780 3307 4762 618 Size: 7 527 1455 1842 Fragments arranged by size: 1842 1455 627 575 527 325 218 217 90 64 39 7 BanII G_rGCy'C Cuts at: 1391 2378 3178 3305 1391 Size: 987 800 127 4072 Fragments arranged by size: 4072 987 800 127 BbeI G_GCGC'C Cuts at: 2366 2405 2366 Size: 39 5947 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2087 2858 4597 Size: 107 122 71 631 605 415 771 1739 Cuts at: 4597 136 Size: 1525 Fragments arranged by size: 1739 1525 771 631 605 415 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 3233 3403 3233 Size: 170 5816 Bme1580I G_kGCm'C Cuts at: 1790 2092 2378 2496 2712 2783 2813 2901 3178 Size: 302 286 118 216 71 30 88 277 Cuts at: 3178 4047 5203 1790 Size: 869 1156 2573 Fragments arranged by size: 2573 1156 869 302 286 277 216 118 88 71 30 BmgBI CAC'GTC Cuts at: 1318 2770 1318 Size: 1452 4534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2715 3717 4675 Size: 416 53 83 399 24 1298 1002 958 Cuts at: 4675 442 Size: 1753 Fragments arranged by size: 1753 1298 1002 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2354 2835 2891 3200 4646 1239 Size: 842 273 481 56 309 1446 2579 Fragments arranged by size: 2579 1446 842 481 309 273 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2059 4111 4976 5274 2059 Size: 2052 865 298 2771 Fragments arranged by size: 2771 2052 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4649 882 Size: 617 3150 2219 Fragments arranged by size: 3150 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2896 3777 493 Size: 711 1692 881 2702 Fragments arranged by size: 2702 1692 881 711 BsaBI GATnn'nnATC Cuts at: 2738 2915 5980 2738 Size: 177 3065 2744 Fragments arranged by size: 3065 2744 177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2044 2363 2402 2520 Size: 53 83 186 936 511 319 39 118 Cuts at: 2520 3793 4175 4810 275 Size: 1273 382 635 1451 Fragments arranged by size: 1451 1273 936 635 511 382 319 186 118 83 53 39 BsaWI w'CCGG_w Cuts at: 3078 4419 4817 5091 5238 5649 3078 Size: 1341 398 274 147 411 3415 Fragments arranged by size: 3415 1341 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5761 Fragments arranged by size: 5761 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 1186 Size: 1024 4962 BseYI C'CCAG_C Cuts at: 2085 5189 5685 2085 Size: 3104 496 2386 Fragments arranged by size: 3104 2386 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 2408 2818 1163 Size: 573 603 69 410 4331 Fragments arranged by size: 4331 603 573 410 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2162 2508 2813 2901 4047 4132 5203 2162 Size: 346 305 88 1146 85 1071 2945 Fragments arranged by size: 2945 1146 1071 346 305 88 85 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 BsmBI CGTCTCn'nnnn_ Cuts at: 2035 2486 2035 Size: 451 5535 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 2162 2378 2496 2508 2712 2783 Size: 399 302 70 216 118 12 204 71 Cuts at: 2783 2813 2901 3178 3305 4047 4132 5203 1391 Size: 30 88 277 127 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 302 277 216 204 127 118 88 85 71 70 30 12 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 2265 2907 3875 5605 2265 Size: 642 968 1730 2646 Fragments arranged by size: 2646 1730 968 642 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3153 3341 844 Size: 2309 188 3489 Fragments arranged by size: 3489 2309 188 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4481 4655 65 Size: 1183 3233 174 1396 Fragments arranged by size: 3233 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 4630 4817 1700 Size: 110 271 2549 187 2869 Fragments arranged by size: 2869 2549 271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 2161 4046 5058 2161 Size: 1885 1012 3089 Fragments arranged by size: 3089 1885 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2329 794 Size: 1535 4451 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2014 3316 3683 4068 4085 5537 5623 5635 Size: 42 1302 367 385 17 1452 86 12 Cuts at: 5635 5981 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1302 385 367 346 86 42 17 12 BstZ17I GTA'TAC Cuts at: 2925 2925 Size: 5986 BtgI C'CryG_G Cuts at: 513 1065 2004 2676 2853 3105 513 Size: 552 939 672 177 252 3394 Fragments arranged by size: 3394 939 672 552 252 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 3237 3502 4296 4316 3237 Size: 265 794 20 4907 Fragments arranged by size: 4907 794 265 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DraIII CAC_nnn'GTG Cuts at: 2787 2787 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2426 2466 Size: 54 1425 50 275 18 249 347 40 Cuts at: 2466 2589 2617 2679 2850 3039 3433 4322 5951 Size: 123 28 62 171 189 394 889 1629 Cuts at: 5951 8 Size: 43 Fragments arranged by size: 1629 1425 889 394 347 275 249 189 171 123 62 54 50 43 40 28 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3916 1353 Size: 2563 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2564 3047 4573 4947 5093 387 Size: 1064 138 975 483 1526 374 146 1280 Fragments arranged by size: 1526 1280 1064 975 483 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2354 2835 2891 3200 4049 4646 Size: 393 449 273 481 56 309 849 597 Cuts at: 4646 5433 5701 1239 Size: 787 268 1524 Fragments arranged by size: 1524 849 787 597 481 449 393 309 273 268 56 EcoO109I rG'GnC_Cy Cuts at: 2034 2374 3175 2034 Size: 340 801 4845 Fragments arranged by size: 4845 801 340 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 EcoRV GAT'ATC Cuts at: 2917 2917 Size: 5986 FspI TGC'GCA Cuts at: 2513 4492 2513 Size: 1979 4007 HaeII r_GCGC'y Cuts at: 1678 2056 2171 2303 2366 2405 2456 2556 5133 Size: 378 115 132 63 39 51 100 2577 Cuts at: 5133 1678 Size: 2531 Fragments arranged by size: 2577 2531 378 132 115 100 63 51 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4627 4659 4701 4733 1373 Size: 32 135 32 3055 32 42 32 2626 Fragments arranged by size: 3055 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3330 3576 669 Size: 988 281 1392 246 3079 Fragments arranged by size: 3079 1392 988 281 246 HindIII A'AGCT_T Cuts at: 748 2594 748 Size: 1846 4140 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KasI G'GCGC_C Cuts at: 2362 2401 2362 Size: 39 5947 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 3051 5862 3051 Size: 2811 3175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2468 2591 10 Size: 54 1425 343 636 123 3405 Fragments arranged by size: 3405 1425 636 343 123 54 MslI CAynn'nnrTG Cuts at: 518 1166 3944 4303 4462 518 Size: 648 2778 359 159 2042 Fragments arranged by size: 2778 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2260 2407 2517 2948 3402 4079 5227 5472 Size: 654 147 110 431 454 677 1148 245 Cuts at: 5472 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 654 454 431 245 147 110 NaeI GCC'GGC Cuts at: 2083 2083 Size: 5986 NarI GG'CG_CC Cuts at: 2363 2402 2363 Size: 39 5947 NcoI C'CATG_G Cuts at: 513 1065 2004 3105 513 Size: 552 939 1101 3394 Fragments arranged by size: 3394 1101 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NgoMIV G'CCGG_C Cuts at: 2081 2081 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 2135 3344 4889 1132 Size: 97 906 1209 1545 2229 Fragments arranged by size: 2229 1545 1209 906 97 PasI CC'CwG_GG Cuts at: 1348 3244 1348 Size: 1896 4090 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2664 1108 Size: 1556 4430 PfoI T'CCnGG_A Cuts at: 3087 3087 Size: 5986 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PmlI CAC'GTG Cuts at: 2896 2896 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PspOMI G'GGCC_C Cuts at: 2374 3174 2374 Size: 800 5186 PstI C_TGCA'G Cuts at: 830 3150 3338 830 Size: 2320 188 3478 Fragments arranged by size: 3478 2320 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2260 2260 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SexAI A'CCwGG_T Cuts at: 2487 2487 Size: 5986 SfcI C'TryA_G Cuts at: 826 1045 3146 3334 4469 5150 5341 826 Size: 219 2101 188 1135 681 191 1471 Fragments arranged by size: 2101 1471 1135 681 219 191 188 SfiI GGCCn_nnn'nGGCC Cuts at: 2858 2858 Size: 5986 SfoI GGC'GCC Cuts at: 2364 2403 2364 Size: 39 5947 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2038 4090 4991 5253 820 Size: 197 926 95 2052 901 262 1553 Fragments arranged by size: 2052 1553 926 901 262 197 95 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 2135 3344 1132 Size: 1003 1209 3774 Fragments arranged by size: 3774 1209 1003 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2004 2443 3105 3282 3425 513 Size: 552 939 439 662 177 143 3074 Fragments arranged by size: 3074 939 662 552 439 177 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 2656 2813 4013 4030 4183 4368 Size: 1255 705 371 157 1200 17 153 185 Cuts at: 4368 4813 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1200 705 445 371 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4232 5944 96 Size: 274 80 33 51 1443 2255 1712 138 Fragments arranged by size: 2255 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2109 2141 5946 5978 2109 Size: 32 3805 32 2117 Fragments arranged by size: 3805 2117 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 4115 4115 Size: 5986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I EcoICRI EcoNI FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SrfI SwaI XcmI