(Linear) (Six-base) MAPSORT of: KIEE4414.seq Check: 8591 from: 1 to: 1281 KIEE4414.orf With 182 enzymes: SgfI * February 2, 2012 14:09 .. AccI GT'mk_AC Cuts at: 0 919 1281 Size: 919 362 AfeI AGC'GCT Cuts at: 0 49 449 1281 Size: 49 400 832 Fragments arranged by size: 832 400 49 AflIII A'CryG_T Cuts at: 0 1046 1281 Size: 1046 235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 853 1281 Size: 853 428 AlwNI CAG_nnn'CTG Cuts at: 0 103 1152 1281 Size: 103 1049 129 Fragments arranged by size: 1049 129 103 ApaI G_GGCC'C Cuts at: 0 373 1173 1281 Size: 373 800 108 Fragments arranged by size: 800 373 108 ApaLI G'TGCA_C Cuts at: 0 804 892 1281 Size: 804 88 389 Fragments arranged by size: 804 389 88 AvaI C'yCGr_G Cuts at: 0 947 1281 Size: 947 334 BanI G'GyrC_C Cuts at: 0 357 396 486 704 768 775 1281 Size: 357 39 90 218 64 7 506 Fragments arranged by size: 506 357 218 90 64 39 7 BanII G_rGCy'C Cuts at: 0 373 1173 1281 Size: 373 800 108 Fragments arranged by size: 800 373 108 BbeI G_GCGC'C Cuts at: 0 361 400 1281 Size: 361 39 881 Fragments arranged by size: 881 361 39 BglI GCCn_nnn'nGGC Cuts at: 0 82 853 1281 Size: 82 771 428 Fragments arranged by size: 771 428 82 BlpI GC'TnA_GC Cuts at: 0 1228 1281 Size: 1228 53 Bme1580I G_kGCm'C Cuts at: 0 87 373 491 707 778 808 896 1173 Size: 87 286 118 216 71 30 88 277 Cuts at: 1173 1281 Size: 108 Fragments arranged by size: 286 277 216 118 108 88 87 71 30 BmgBI CAC'GTC Cuts at: 0 765 1281 Size: 765 516 BmrI ACTGGGnnnn_n' Cuts at: 0 710 1281 Size: 710 571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 349 830 886 1195 1281 Size: 76 273 481 56 309 86 Fragments arranged by size: 481 309 273 86 76 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 1281 Size: 54 1227 BsaAI yAC'GTr Cuts at: 0 891 1281 Size: 891 390 BsaBI GATnn'nnATC Cuts at: 0 733 910 1281 Size: 733 177 371 Fragments arranged by size: 733 371 177 BsaHI Gr'CG_yC Cuts at: 0 39 358 397 515 1281 Size: 39 319 39 118 766 Fragments arranged by size: 766 319 118 39 39 BsaWI w'CCGG_w Cuts at: 0 1073 1281 Size: 1073 208 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 205 1281 Size: 205 1076 BseYI C'CCAG_C Cuts at: 0 80 1281 Size: 80 1201 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 403 813 1281 Size: 334 69 410 468 Fragments arranged by size: 468 410 334 69 BsiHKAI G_wGCw'C Cuts at: 0 157 503 808 896 1281 Size: 157 346 305 88 385 Fragments arranged by size: 385 346 305 157 88 BsmBI CGTCTCn'nnnn_ Cuts at: 0 30 481 1281 Size: 30 451 800 Fragments arranged by size: 800 451 30 Bsp1286I G_dGCh'C Cuts at: 0 87 157 373 491 503 707 778 808 Size: 87 70 216 118 12 204 71 30 Cuts at: 808 896 1173 1281 Size: 88 277 108 Fragments arranged by size: 277 216 204 118 108 88 87 71 70 30 12 BspHI T'CATG_A Cuts at: 0 260 902 1281 Size: 260 642 379 Fragments arranged by size: 642 379 260 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1148 1281 Size: 1148 133 BsrFI r'CCGG_y Cuts at: 0 76 1281 Size: 76 1205 BssSI C'ACGA_G Cuts at: 0 156 1281 Size: 156 1125 BstAPI GCAn_nnn'nTGC Cuts at: 0 324 1281 Size: 324 957 BstYI r'GATC_y Cuts at: 0 9 1281 Size: 9 1272 BstZ17I GTA'TAC Cuts at: 0 920 1281 Size: 920 361 BtgI C'CryG_G Cuts at: 0 671 848 1100 1281 Size: 671 177 252 181 Fragments arranged by size: 671 252 181 177 BtsI GCAGTG_nn' Cuts at: 0 1232 1281 Size: 1232 49 DraIII CAC_nnn'GTG Cuts at: 0 782 1281 Size: 782 499 EaeI y'GGCC_r Cuts at: 0 74 421 461 584 612 674 845 1034 Size: 74 347 40 123 28 62 171 189 Cuts at: 1034 1281 Size: 247 Fragments arranged by size: 347 247 189 171 123 74 62 40 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 559 1042 1281 Size: 559 483 239 Fragments arranged by size: 559 483 239 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 349 830 886 1195 1281 Size: 76 273 481 56 309 86 Fragments arranged by size: 481 309 273 86 76 56 EcoO109I rG'GnC_Cy Cuts at: 0 29 369 1170 1281 Size: 29 340 801 111 Fragments arranged by size: 801 340 111 29 EcoRV GAT'ATC Cuts at: 0 912 1281 Size: 912 369 FspI TGC'GCA Cuts at: 0 508 1281 Size: 508 773 HaeII r_GCGC'y Cuts at: 0 51 166 298 361 400 451 551 1281 Size: 51 115 132 63 39 51 100 730 Fragments arranged by size: 730 132 115 100 63 51 51 39 HindIII A'AGCT_T Cuts at: 0 589 1281 Size: 589 692 KasI G'GCGC_C Cuts at: 0 357 396 1281 Size: 357 39 885 Fragments arranged by size: 885 357 39 MluI A'CGCG_T Cuts at: 0 1046 1281 Size: 1046 235 MscI TGG'CCA Cuts at: 0 463 586 1281 Size: 463 123 695 Fragments arranged by size: 695 463 123 MspA1I CmG'CkG Cuts at: 0 255 402 512 943 1281 Size: 255 147 110 431 338 Fragments arranged by size: 431 338 255 147 110 NaeI GCC'GGC Cuts at: 0 78 1281 Size: 78 1203 NarI GG'CG_CC Cuts at: 0 358 397 1281 Size: 358 39 884 Fragments arranged by size: 884 358 39 NcoI C'CATG_G Cuts at: 0 1100 1281 Size: 1100 181 NgoMIV G'CCGG_C Cuts at: 0 76 1281 Size: 76 1205 NspI r_CATG'y Cuts at: 0 130 1281 Size: 130 1151 PasI CC'CwG_GG Cuts at: 0 1239 1281 Size: 1239 42 PflMI CCAn_nnn'nTGG Cuts at: 0 659 1281 Size: 659 622 PfoI T'CCnGG_A Cuts at: 0 1082 1281 Size: 1082 199 PmlI CAC'GTG Cuts at: 0 891 1281 Size: 891 390 PspOMI G'GGCC_C Cuts at: 0 369 1169 1281 Size: 369 800 112 Fragments arranged by size: 800 369 112 PstI C_TGCA'G Cuts at: 0 1145 1281 Size: 1145 136 PvuII CAG'CTG Cuts at: 0 255 1281 Size: 255 1026 SexAI A'CCwGG_T Cuts at: 0 482 1281 Size: 482 799 SfcI C'TryA_G Cuts at: 0 1141 1281 Size: 1141 140 SfiI GGCCn_nnn'nGGCC Cuts at: 0 853 1281 Size: 853 428 SfoI GGC'GCC Cuts at: 0 359 398 1281 Size: 359 39 883 Fragments arranged by size: 883 359 39 SmlI C'TyrA_G Cuts at: 0 33 1281 Size: 33 1248 SphI G_CATG'C Cuts at: 0 130 1281 Size: 130 1151 StyI C'CwwG_G Cuts at: 0 438 1100 1281 Size: 438 662 181 Fragments arranged by size: 662 438 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 280 651 808 1281 Size: 280 371 157 473 Fragments arranged by size: 473 371 280 157 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 104 136 1281 Size: 104 32 1145 Fragments arranged by size: 1145 104 32 Enzymes that do cut: AccI AfeI AflIII AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BssSI BstAPI BstYI BstZ17I BtgI BtsI DraIII EaeI EciI Eco57MI EcoO109I EcoRV FspI HaeII HindIII KasI MluI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PspOMI PstI PvuII SexAI SfcI SfiI SfoI SmlI SphI StyI TaqII TstI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmtI BplI Bpu10I BsaI BsaXI BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoNI EcoRI FalI FseI FspAI Hin4I HincII HpaI KpnI MfeI MmeI MslI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI