################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AccI AclI AgeI AscI Asp700I AvrII BbeI BlnI BsiWI BspDI BspHI BssHII Bst1107I BstZ17I ClaI CpoI EcoNI FseI HpaI KasI MfeI MluI MunI NarI NheI NotI PacI PaeR7I PinAI PmeI Psp1406I PvuI RcaI RsrII SalI SbfI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 3091 GGTACC G^GTACC AccIII 606 TCCGGA T^CCGGA AflII 4593 CTTAAG C^TTAAG AhdI 4138 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 1546 ATTAAT AT^TAAT AsnI 1546 ATTAAT AT^TAAT Asp718I 3091 GGTACC G^GTACC AspEI 4138 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 764 TGCGCA TGC^GCA BalI 735 TGGCCA TGG^CCA BbrPI 2412 CACGTG CAC^GTG BciVI 2784 GGATAC GTATCC(6/5) BfaI 814 CTAG C^TAG BfrI 4593 CTTAAG C^TTAAG BglII 316 AGATCT A^GATCT BseAI 606 TCCGGA T^CCGGA BspEI 606 TCCGGA T^CCGGA BspLU11I 2406 ACATGT A^CATGT BstBI 5359 TTCGAA TT^CGAA BstEII 4551 GGTCACC G^GT[ACGT]ACC BstPI 4551 GGTCACC G^GT[ACGT]ACC BstXI 7421 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 496 TTTAAA TTT^AAA Eam1105I 4138 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4551 GGTCACC G^GT[ACGT]ACC EcoRI 328 GAATTC G^AATTC EcoT22I 2477 ATGCAT ATGCA^T FspI 764 TGCGCA TGC^GCA KpnI 3091 GGTACC GGTAC^C MaeI 814 CTAG C^TAG MluNI 735 TGGCCA TGG^CCA MroI 606 TCCGGA T^CCGGA MscI 735 TGGCCA TGG^CCA NaeI 13 GCCGGC GCC^GGC NcoI 2387 CCATGG C^CATGG NdeI 6576 CATATG CA^TATG NgoMIV 13 GCCGGC G^CCGGC NruI 5484 TCGCGA TCG^CGA NsiI 2477 ATGCAT ATGCA^T NspV 5359 TTCGAA TT^CGAA PmaCI 2412 CACGTG CAC^GTG PmlI 2412 CACGTG CAC^GTG PshBI 1546 ATTAAT AT^TAAT SexAI 4754 ACCAGGT A^CC[AT]GGT SfuI 5359 TTCGAA TT^CGAA StuI 4843 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 3802 GACGTC GACGT^C 6157 GACGTC AocI 4846 CCTGAGG CC^T[ACGT]AGG 6385 CCTCAGG BbvCI 6821 CCTCAGC CCTCAGC(-5/-2) 7194 CCTCAGC BclI 566 TGATCA T^GATCA 4826 TGATCA BlpI 5249 GCTTAGC GC^T[ACGT]AGC 6927 GCTCAGC Bpu1102I 5249 GCTTAGC GC^T[ACGT]AGC 6927 GCTCAGC BsaBI 553 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2474 GATATGCATC BsmBI 2871 CGTCTC CGTCTC(1/5) 2198 GAGACG Bsp1407I 4772 TGTACA T^GTACA 6286 TGTACA BsrFI 13 GCCGGC [GA]^CCGG[CT] 2563 ACCGGC BsrGI 4772 TGTACA T^GTACA 6286 TGTACA Bsu36I 4846 CCTGAGG CC^T[ACGT]AGG 6385 CCTCAGG CelII 5249 GCTTAGC GC^T[ACGT]AGC 6927 GCTCAGC Cfr10I 13 GCCGGC [GA]^CCGG[CT] 2563 ACCGGC EagI 15 CGGCCG C^GGCCG 5172 CGGCCG EclXI 15 CGGCCG C^GGCCG 5172 CGGCCG Eco52I 15 CGGCCG C^GGCCG 5172 CGGCCG Eco81I 4846 CCTGAGG CC^T[ACGT]AGG 6385 CCTCAGG FbaI 566 TGATCA T^GATCA 4826 TGATCA KspI 3482 CCGCGG CCGC^GG 4002 CCGCGG MamI 553 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2474 GATATGCATC NspI 2406 ACATGT [GA]CATG^[CT] 4897 ACATGC PflMI 3694 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6162 CCAGGTCCTGG PshAI 4375 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5370 GACTCCTGTC SacII 3482 CCGCGG CCGC^GG 4002 CCGCGG ScaI 5239 AGTACT AGT^ACT 6641 AGTACT SspBI 4772 TGTACA T^GTACA 6286 TGTACA Van91I 3694 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6162 CCAGGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AspHI AspI AvaI AvaII BamHI BanI BanII BbsI BbvI BcgI BcnI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmaCI XmaI