################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeII MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TspRI Tth111I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 962 GTCGAC GT^[AC][GT]AC Alw44I 1094 GTGCAC G^TGCAC ApaLI 1094 GTGCAC G^TGCAC Asp700I 308 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 383 GGCACC G^G[CT][GA]CC BbvI 190 GCTGC GCAGC(8/12) BglII 328 AGATCT A^GATCT BpmI 606 CTGGAG CTGGAG(16/14) BsaBI 655 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 282 GAGACC GGTCTC(1/5) BseRI 482 CTCCTC GAGGAG(10/8) BsmI 186 GAATGC GAATGC(1/-1) BspHI 996 TCATGA T^CATGA BsrI 445 CCAGT ACTGG(1/-1) BstAPI 1096 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 328 AGATCT [GA]^GATC[CT] Cac8I 23 GCAAGC GC[ACGT]^[ACGT]GC EarI 560 GAAGAG CTCTTC(1/4) EcoNI 1102 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 190 GCTGC GC^[ACGT]GC HindIII 25 AAGCTT A^AGCTT ItaI 190 GCTGC GC^[ACGT]GC Ksp632I 560 GAAGAG CTCTTC(1/4) MamI 655 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 328 AGATCT [GA]^GATC[CT] NcoI 933 CCATGG C^CATGG PstI 173 CTGCAG CTGCA^G RcaI 996 TCATGA T^CATGA SalI 962 GTCGAC G^TCGAC TfiI 1254 GAATC G^A[AT]TC TseI 190 GCTGC G^C[AT]GC Tsp45I 552 GTCAC ^GT[GC]AC Tsp509I 568 AATT ^AATT XhoII 328 AGATCT [GA]^GATC[CT] XmnI 308 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 1258 CCGC CCGC(-3/-1) 279 GCGG AlwI 1285 GATCC GGATC(4/5) 123 GGATC AspHI 1094 GTGCAC G[AT]GC[AT]^C 1276 GTGCTC BsiHKAI 1094 GTGCAC G[AT]GC[AT]^C 1276 GTGCTC BsmAI 282 GAGAC GTCTC(1/5) 1361 GAGAC BspMI 1101 ACCTGC ACCTGC(4/8) 1138 ACCTGC BssSI 1160 CACGAG CACGAG(-5/-1) 1289 CTCGTG DsaI 345 CCGTGG C^C[GA][CT]GG 933 CCATGG EcoT22I 713 ATGCAT ATGCA^T 824 ATGCAT HincII 624 GTCAAC GT[CT]^[GA]AC 962 GTCGAC HindII 624 GTCAAC GT[CT]^[GA]AC 962 GTCGAC HphI 1009 GGTGA GGTGA(8/7) 1204 GGTGA MaeIII 552 GTCAC ^GT[ACGT]AC 1190 GTTAC MseI 71 TTAA T^TAA 449 TTAA NsiI 713 ATGCAT ATGCA^T 824 ATGCAT PaeR7I 1194 CTCGAG C^TCGAG 1365 CTCGAG SfcI 173 CTGCAG C^T[GA][CT]AG 1325 CTATAG Tru9I 71 TTAA T^TAA 449 TTAA XhoI 1194 CTCGAG C^TCGAG 1365 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AluI AvaI AvaII BciVI BfaI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FokI HaeIII HinfI MaeI MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TthHB8I XbaI