(Circular) (Six-base) MAPSORT of: pFN21AE5834.seq Check: 2393 from: 1 to: 6082 pFN21AE5834 6082 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3115 3152 3115 Size: 37 6045 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3892 278 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2968 3425 1937 Size: 1031 457 4594 Fragments arranged by size: 4594 1031 457 Acc65I G'GTAC_C Cuts at: 3403 3403 Size: 6082 AclI AA'CG_TT Cuts at: 1563 4209 4582 1563 Size: 2646 373 3063 Fragments arranged by size: 3063 2646 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2304 3059 4145 5529 5797 1632 Size: 672 755 1086 1384 268 1917 Fragments arranged by size: 1917 1384 1086 755 672 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5885 AflIII A'CryG_T Cuts at: 1203 4981 5958 1203 Size: 3778 977 1327 Fragments arranged by size: 3778 1327 977 AgeI A'CCGG_T Cuts at: 4913 4913 Size: 6082 AhdI GACnn_n'nnGTC Cuts at: 4811 4811 Size: 6082 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6082 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6082 AlwNI CAG_nnn'CTG Cuts at: 3825 5397 3825 Size: 1572 4510 ApaLI G'TGCA_C Cuts at: 3099 4139 5295 3099 Size: 1040 1156 3886 Fragments arranged by size: 3886 1156 1040 ApoI r'AATT_y Cuts at: 1493 3391 3617 3769 5878 1493 Size: 1898 226 152 2109 1697 Fragments arranged by size: 2109 1898 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4636 160 Size: 872 3604 1606 Fragments arranged by size: 3604 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 AvaI C'yCGr_G Cuts at: 1870 1943 2790 2901 3199 3370 3407 1870 Size: 73 847 111 298 171 37 4545 Fragments arranged by size: 4545 847 298 171 111 73 37 BamHI G'GATC_C Cuts at: 3412 3779 3412 Size: 367 5715 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2388 3403 4858 618 Size: 325 217 627 601 1015 1455 1842 Fragments arranged by size: 1842 1455 1015 627 601 325 217 BanII G_rGCy'C Cuts at: 1391 3401 1391 Size: 2010 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4273 4307 1903 Size: 34 2336 34 3678 Fragments arranged by size: 3678 2336 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2399 2873 2936 3974 5184 2399 Size: 474 63 1038 1210 3297 Fragments arranged by size: 3297 1210 1038 474 63 BclI T'GATC_A Cuts at: 1548 1548 Size: 6082 BfrBI ATG'CAT Cuts at: 2720 2831 2720 Size: 111 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4693 136 Size: 107 122 71 631 605 3021 1525 Fragments arranged by size: 3021 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2333 6077 2333 Size: 3744 2338 BlpI GC'TnA_GC Cuts at: 3499 3499 Size: 6082 Bme1580I G_kGCm'C Cuts at: 1790 2391 3103 4143 5299 1790 Size: 601 712 1040 1156 2573 Fragments arranged by size: 2573 1156 1040 712 601 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6082 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3813 4771 442 Size: 416 53 83 399 24 2396 958 1753 Fragments arranged by size: 2396 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6082 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3214 3214 Size: 6082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2632 4742 1239 Size: 1393 2110 2579 Fragments arranged by size: 2579 2110 1393 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2405 3298 4207 5072 5370 2405 Size: 893 909 865 298 3117 Fragments arranged by size: 3117 909 893 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2281 4745 882 Size: 617 782 2464 2219 Fragments arranged by size: 2464 2219 782 617 BsaAI yAC'GTr Cuts at: 493 1204 3873 493 Size: 711 2669 2702 Fragments arranged by size: 2702 2669 711 BsaBI GATnn'nnATC Cuts at: 2664 6076 2664 Size: 3412 2670 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3889 4271 4906 275 Size: 53 83 186 936 2356 382 635 1451 Fragments arranged by size: 2356 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4515 4913 5187 5334 5745 4515 Size: 398 274 147 411 4852 Fragments arranged by size: 4852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5857 Fragments arranged by size: 5857 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2478 1186 Size: 1292 4790 BseYI C'CCAG_C Cuts at: 5285 5781 5285 Size: 496 5586 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5509 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3532 4293 5321 1540 Size: 275 186 1531 761 1028 2301 Fragments arranged by size: 2301 1531 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3103 3285 4143 4228 5299 3103 Size: 182 858 85 1071 3886 Fragments arranged by size: 3886 1071 858 182 85 BsmI GAATG_Cn' Cuts at: 2197 3593 3686 2197 Size: 1396 93 4593 Fragments arranged by size: 4593 1396 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 3103 3285 3401 4143 4228 5299 Size: 399 601 712 182 116 742 85 1071 Cuts at: 5299 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 712 601 399 182 116 85 BspEI T'CCGG_A Cuts at: 5745 5745 Size: 6082 BspHI T'CATG_A Cuts at: 3001 3971 5701 3001 Size: 970 1730 3382 Fragments arranged by size: 3382 1730 970 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3115 3152 3437 844 Size: 2271 37 285 3489 Fragments arranged by size: 3489 2271 285 37 BsrBI CCG'CTC Cuts at: 1394 1823 3969 1394 Size: 429 2146 3507 Fragments arranged by size: 3507 2146 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4577 4751 65 Size: 1183 3329 174 1396 Fragments arranged by size: 3329 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4726 4913 1700 Size: 110 2916 187 2869 Fragments arranged by size: 2916 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6082 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6082 BssSI C'ACGA_G Cuts at: 3165 3294 4142 5154 3165 Size: 129 848 1012 4093 Fragments arranged by size: 4093 1012 848 129 BstAPI GCAn_nnn'nTGC Cuts at: 794 3107 794 Size: 2313 3769 BstBI TT'CG_AA Cuts at: 4886 4886 Size: 6082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6082 BstYI r'GATC_y Cuts at: 1972 2333 3412 3779 4164 4181 5633 5719 5731 Size: 361 1079 367 385 17 1452 86 12 Cuts at: 5731 6077 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1079 385 367 361 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2350 2938 513 Size: 552 939 346 588 3657 Fragments arranged by size: 3657 939 588 552 346 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5343 Fragments arranged by size: 5343 683 56 BtsI GCAGTG_nn' Cuts at: 3598 4392 4412 3598 Size: 794 20 5268 Fragments arranged by size: 5268 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6082 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3303 3338 529 Size: 35 2739 35 3273 Fragments arranged by size: 3273 2739 35 35 DraI TTT'AAA Cuts at: 3386 3386 Size: 6082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5089 809 Size: 507 3773 1802 Fragments arranged by size: 3773 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3529 4418 6047 Size: 54 1425 50 275 18 1699 889 1629 Cuts at: 6047 8 Size: 43 Fragments arranged by size: 1699 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3529 1537 Size: 275 1717 4090 Fragments arranged by size: 4090 1717 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2560 4012 1353 Size: 1207 1452 3423 Fragments arranged by size: 3423 1452 1207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2299 3252 4669 5043 5189 387 Size: 1064 138 710 953 1417 374 146 1280 Fragments arranged by size: 1417 1280 1064 953 710 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2304 2632 3059 4145 4742 5529 5797 Size: 393 672 328 427 1086 597 787 268 Cuts at: 5797 1239 Size: 1524 Fragments arranged by size: 1524 1086 787 672 597 427 393 328 268 EcoNI CCTnn'n_nnAGG Cuts at: 3111 3111 Size: 6082 EcoO109I rG'GnC_Cy Cuts at: 2483 2549 3019 3087 3116 2483 Size: 66 470 68 29 5449 Fragments arranged by size: 5449 470 68 66 29 EcoRI G'AATT_C Cuts at: 3391 3769 3391 Size: 378 5704 FspI TGC'GCA Cuts at: 4588 4588 Size: 6082 HaeII r_GCGC'y Cuts at: 1678 5229 1678 Size: 3551 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2145 2605 2612 2637 Size: 32 135 32 541 32 460 7 25 Cuts at: 2637 2644 3338 3370 4723 4755 4797 4829 1373 Size: 7 694 32 1353 32 42 32 2626 Fragments arranged by size: 2626 1353 694 541 460 135 42 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2631 2969 3426 3672 669 Size: 988 281 693 338 457 246 3079 Fragments arranged by size: 3079 988 693 457 338 281 246 HindIII A'AGCT_T Cuts at: 748 2030 748 Size: 1282 4800 HpaI GTT'AAC Cuts at: 3672 3672 Size: 6082 KpnI G_GTAC'C Cuts at: 3407 3407 Size: 6082 MfeI C'AATT_G Cuts at: 3681 3681 Size: 6082 MluI A'CGCG_T Cuts at: 5958 5958 Size: 6082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2513 5196 5380 1305 Size: 1208 2683 184 2007 Fragments arranged by size: 2683 2007 1208 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4260 Fragments arranged by size: 4260 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2394 3048 3222 4040 4399 4558 518 Size: 648 1228 654 174 818 359 159 2042 Fragments arranged by size: 2042 1228 818 654 648 359 174 159 MspA1I CmG'CkG Cuts at: 1606 3498 4175 5323 5568 1606 Size: 1892 677 1148 245 2120 Fragments arranged by size: 2120 1892 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2938 513 Size: 552 939 934 3657 Fragments arranged by size: 3657 939 934 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6082 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6082 NotI GC'GGCC_GC Cuts at: 3529 3529 Size: 6082 NsiI A_TGCA'T Cuts at: 2722 2833 2722 Size: 111 5971 NspI r_CATG'y Cuts at: 1132 1229 2629 3239 3306 3440 4985 1132 Size: 97 1400 610 67 134 1545 2229 Fragments arranged by size: 2229 1545 1400 610 134 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6082 PciI A'CATG_T Cuts at: 4981 4981 Size: 6082 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6082 PfoI T'CCnGG_A Cuts at: 2609 2609 Size: 6082 PmeI GTTT'AAAC Cuts at: 3386 3386 Size: 6082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4151 4183 5664 5696 4151 Size: 32 1481 32 4537 Fragments arranged by size: 4537 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2483 2549 3087 2483 Size: 66 538 5478 Fragments arranged by size: 5478 538 66 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6082 PsiI TTA'TAA Cuts at: 3652 3652 Size: 6082 PspXI vC'TCGA_Gb Cuts at: 3199 3370 3199 Size: 171 5911 PstI C_TGCA'G Cuts at: 830 2182 3434 830 Size: 1352 1252 3478 Fragments arranged by size: 3478 1352 1252 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6082 SalI G'TCGA_C Cuts at: 1936 2967 3424 1936 Size: 1031 457 4594 Fragments arranged by size: 4594 1031 457 SbfI CC_TGCA'GG Cuts at: 3434 3434 Size: 6082 ScaI AGT'ACT Cuts at: 4330 6042 4330 Size: 1712 4370 SfcI C'TryA_G Cuts at: 826 1045 2178 3330 3430 4565 5246 5437 826 Size: 219 1133 1152 100 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 1133 681 219 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6082 SmaI CCC'GGG Cuts at: 1872 3409 1872 Size: 1537 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 3199 3313 3370 4186 5087 Size: 197 926 477 779 114 57 816 901 Cuts at: 5087 5349 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 816 779 477 262 197 114 57 SnaBI TAC'GTA Cuts at: 493 3873 493 Size: 3380 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6082 SphI G_CATG'C Cuts at: 1132 3440 1132 Size: 2308 3774 SspI AAT'ATT Cuts at: 5 52 4006 5 Size: 47 3954 2081 Fragments arranged by size: 3954 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6082 StyI C'CwwG_G Cuts at: 513 1065 2004 2545 2938 3022 3521 513 Size: 552 939 541 393 84 499 3074 Fragments arranged by size: 3074 939 552 541 499 393 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4109 4126 4279 4464 4909 325 Size: 1255 2529 17 153 185 445 1498 Fragments arranged by size: 2529 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4328 6040 96 Size: 274 80 33 51 1443 2351 1712 138 Fragments arranged by size: 2351 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6042 6074 6042 Size: 32 6050 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6082 XbaI T'CTAG_A Cuts at: 2708 2762 2819 2873 2930 2984 3418 2708 Size: 54 57 54 57 54 434 5372 Fragments arranged by size: 5372 434 57 57 54 54 54 XhoI C'TCGA_G Cuts at: 1943 3199 3370 1943 Size: 1256 171 4655 Fragments arranged by size: 4655 1256 171 XmaI C'CCGG_G Cuts at: 1870 3407 1870 Size: 1537 4545 XmnI GAAnn'nnTTC Cuts at: 2317 4211 2317 Size: 1894 4188 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3890 276 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI