Restriction map for
pFN21AE5834
(Linear) (Six-base) MAPSORT of: KIEE5834.seq Check: 3787 from: 1 to: 1377
KIEE5834.orf
With 182 enzymes: SgfI *
December 5, 2014 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1110 1147 1377
Size: 1110 37 230
Fragments arranged by size:
1110 230 37
AccI GT'mk_AC
Cuts at: 0 963 1377
Size: 963 414
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1054 1377
Size: 299 755 323
Fragments arranged by size:
755 323 299
ApaLI G'TGCA_C
Cuts at: 0 1094 1377
Size: 1094 283
AvaI C'yCGr_G
Cuts at: 0 785 896 1194 1365 1377
Size: 785 111 298 171 12
Fragments arranged by size:
785 298 171 111 12
BanI G'GyrC_C
Cuts at: 0 383 1377
Size: 383 994
BciVI GTATCCnnnnn_n'
Cuts at: 0 394 868 931 1377
Size: 394 474 63 446
Fragments arranged by size:
474 446 394 63
BfrBI ATG'CAT
Cuts at: 0 715 826 1377
Size: 715 111 551
Fragments arranged by size:
715 551 111
BglII A'GATC_T
Cuts at: 0 328 1377
Size: 328 1049
Bme1580I G_kGCm'C
Cuts at: 0 386 1098 1377
Size: 386 712 279
Fragments arranged by size:
712 386 279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1209 1377
Size: 1209 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1377
Size: 627 750
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1293 1377
Size: 400 893 84
Fragments arranged by size:
893 400 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 276 1377
Size: 276 1101
BsaBI GATnn'nnATC
Cuts at: 0 659 1377
Size: 659 718
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 473 1377
Size: 473 904
BsiHKAI G_wGCw'C
Cuts at: 0 1098 1280 1377
Size: 1098 182 97
Fragments arranged by size:
1098 182 97
BsmI GAATG_Cn'
Cuts at: 0 192 1377
Size: 192 1185
Bsp1286I G_dGCh'C
Cuts at: 0 386 1098 1280 1377
Size: 386 712 182 97
Fragments arranged by size:
712 386 182 97
BspHI T'CATG_A
Cuts at: 0 996 1377
Size: 996 381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1110 1147 1377
Size: 1110 37 230
Fragments arranged by size:
1110 230 37
BssSI C'ACGA_G
Cuts at: 0 1160 1289 1377
Size: 1160 129 88
Fragments arranged by size:
1160 129 88
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1102 1377
Size: 1102 275
BstYI r'GATC_y
Cuts at: 0 328 1377
Size: 328 1049
BtgI C'CryG_G
Cuts at: 0 345 933 1377
Size: 345 588 444
Fragments arranged by size:
588 444 345
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1298 1333 1377
Size: 1298 35 44
Fragments arranged by size:
1298 44 35
EarI CTCTTCn'nnn_
Cuts at: 0 555 1377
Size: 555 822
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 294 1247 1377
Size: 294 953 130
Fragments arranged by size:
953 294 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 627 1054 1377
Size: 299 328 427 323
Fragments arranged by size:
427 328 323 299
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1106 1377
Size: 1106 271
EcoO109I rG'GnC_Cy
Cuts at: 0 478 544 1014 1082 1111 1377
Size: 478 66 470 68 29 266
Fragments arranged by size:
478 470 266 68 66 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 108 140 600 607 632 639 1333 1365
Size: 108 32 460 7 25 7 694 32
Cuts at: 1365 1377
Size: 12
Fragments arranged by size:
694 460 108 32 32 25 12 7
7
HincII GTy'rAC
Cuts at: 0 626 964 1377
Size: 626 338 413
Fragments arranged by size:
626 413 338
HindIII A'AGCT_T
Cuts at: 0 25 1377
Size: 25 1352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1377
Size: 508 869
MslI CAynn'nnrTG
Cuts at: 0 389 1043 1217 1377
Size: 389 654 174 160
Fragments arranged by size:
654 389 174 160
NcoI C'CATG_G
Cuts at: 0 933 1377
Size: 933 444
NsiI A_TGCA'T
Cuts at: 0 717 828 1377
Size: 717 111 549
Fragments arranged by size:
717 549 111
NspI r_CATG'y
Cuts at: 0 624 1234 1301 1377
Size: 624 610 67 76
Fragments arranged by size:
624 610 76 67
PfoI T'CCnGG_A
Cuts at: 0 604 1377
Size: 604 773
PpuMI rG'GwC_Cy
Cuts at: 0 478 544 1082 1377
Size: 478 66 538 295
Fragments arranged by size:
538 478 295 66
PspXI vC'TCGA_Gb
Cuts at: 0 1194 1365 1377
Size: 1194 171 12
Fragments arranged by size:
1194 171 12
PstI C_TGCA'G
Cuts at: 0 177 1377
Size: 177 1200
SalI G'TCGA_C
Cuts at: 0 962 1377
Size: 962 415
SfcI C'TryA_G
Cuts at: 0 173 1325 1377
Size: 173 1152 52
Fragments arranged by size:
1152 173 52
SmlI C'TyrA_G
Cuts at: 0 415 1194 1308 1365 1377
Size: 415 779 114 57 12
Fragments arranged by size:
779 415 114 57 12
StyI C'CwwG_G
Cuts at: 0 540 933 1017 1377
Size: 540 393 84 360
Fragments arranged by size:
540 393 360 84
XbaI T'CTAG_A
Cuts at: 0 703 757 814 868 925 979 1377
Size: 703 54 57 54 57 54 398
Fragments arranged by size:
703 398 57 57 54 54 54
XhoI C'TCGA_G
Cuts at: 0 1194 1365 1377
Size: 1194 171 12
Fragments arranged by size:
1194 171 12
XmnI GAAnn'nnTTC
Cuts at: 0 312 1377
Size: 312 1065
Enzymes that do cut:
AarI AccI AcuI ApaLI AvaI BanI BciVI BfrBI
BglII Bme1580I BplI BpmI BpuEI BsaI BsaBI BseRI
BsiHKAI BsmI Bsp1286I BspHI BspMI BssSI BstAPI BstYI
BtgI CspCI EarI EciI Eco57MI EcoNI EcoO109I Hin4I
HincII HindIII MmeI MslI NcoI NsiI NspI PfoI
PpuMI PspXI PstI SalI SfcI SmlI StyI XbaI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BclI BglI BlpI BmgBI BmrI BmtI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XcmI
XmaI ZraI