Restriction map for
pFN21AE6515
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# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MluI MroI MseI MslI MspA1I
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 156 CGCG CG^CG
AfaI 153 GTAC GT^AC
Aor51HI 85 AGCGCT AGC^GCT
AspHI 348 GAGCTC G[AT]GC[AT]^C
BanII 348 GAGCTC G[GA]GC[CT]^C
BbsI 12 GAAGAC GAAGAC(2/6)
BfaI 58 CTAG C^TAG
BmyI 348 GAGCTC G[AGT]GC[ACT]^C
BpmI 295 CTGGAG CTGGAG(16/14)
BpuAI 12 GAAGAC GAAGAC(2/6)
BsaI 452 GGTCTC GGTCTC(1/5)
BsiHKAI 348 GAGCTC G[AT]GC[AT]^C
BsmAI 453 GTCTC GTCTC(1/5)
Bsp1286I 348 GAGCTC G[AGT]GC[ACT]^C
BstUI 156 CGCG CG^CG
BstYI 109 GGATCT [GA]^GATC[CT]
DraII 144 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 191 CCATGG C^C[GA][CT]GG
Eco47III 85 AGCGCT AGC^GCT
EcoO109I 144 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 191 CCATGG C^C[AT][AT]GG
FauI 387 GCGGG CCCGC(4/6)
MaeI 58 CTAG C^TAG
MflI 109 GGATCT [GA]^GATC[CT]
MvnI 156 CGCG CG^CG
NcoI 191 CCATGG C^CATGG
PaeR7I 140 CTCGAG C^TCGAG
PstI 441 CTGCAG CTGCA^G
RsaI 153 GTAC GT^AC
SacI 348 GAGCTC GAGCT^C
SmlI 140 CTCGAG C^T[CT][GA]AG
StyI 191 CCATGG C^C[AT][AT]GG
TaqI 141 TCGA T^CGA
TthHB8I 141 TCGA T^CGA
XhoI 140 CTCGAG C^TCGAG
XhoII 109 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 157 GCGG CCGC(-3/-1)
387 GCGG
AlwI 109 GGATC GGATC(4/5)
426 GGATC
AvaI 140 CTCGAG C^[CT]CG[GA]G
287 CTCGGG
BalI 5 TGGCCA TGG^CCA
302 TGGCCA
BsaJI 148 CCCAGG C^C[ACGT][ACGT]GG
191 CCATGG
BseRI 325 GAGGAG GAGGAG(10/8)
328 GAGGAG
BsoBI 140 CTCGAG C^[CT]CG[GA]G
287 CTCGGG
BssKI 45 CCTGG ^CC[ACGT]GG
149 CCAGG
BstNI 45 CCTGG CC^[AT]GG
149 CCAGG
CfoI 86 GCGC GCG^C
292 GCGC
Cfr13I 145 GGCCC G^G[ACGT]CC
234 GGCCC
DdeI 119 CTAAG C^T[ACGT]AG
448 CTCAG
DpnII 110 GATC ^GATC
427 GATC
EaeI 5 TGGCCA [CT]^GGCC[GA]
302 TGGCCA
EcoRII 45 CCTGG ^CC[AT]GG
149 CCAGG
HaeII 85 AGCGCT [GA]GCGC^[CT]
291 GGCGCT
HhaI 86 GCGC GCG^C
292 GCGC
HinP1I 86 GCGC G^CGC
292 GCGC
HinfI 201 GATTC G^A[ACGT]TC
399 GATTC
MaeIII 77 GTCAC ^GT[ACGT]AC
176 GTGAC
MboI 110 GATC ^GATC
427 GATC
MluNI 5 TGGCCA TGG^CCA
302 TGGCCA
MscI 5 TGGCCA TGG^CCA
302 TGGCCA
MvaI 45 CCTGG CC^[AT]GG
149 CCAGG
MwoI 304 GCCAGTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
387 GCGGGACTTGC
NdeII 110 GATC ^GATC
427 GATC
NlaIII 115 CATG CATG^
192 CATG
NlaIV 145 GGCCCC GG[ACGT]^[ACGT]CC
339 GGAGCC
Sau3AI 110 GATC ^GATC
427 GATC
Sau96I 145 GGCCC G^G[ACGT]CC
234 GGCCC
ScrFI 45 CCTGG CC^[ACGT]GG
149 CCAGG
SfcI 180 CTACAG C^T[GA][CT]AG
441 CTGCAG
TfiI 201 GATTC G^A[AT]TC
399 GATTC
Tsp45I 77 GTCAC ^GT[GC]AC
176 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsmFI
BsrI Cac8I Fnu4HI HaeIII HphI
ItaI MboII MnlI TseI TspRI