(Linear) (Six-base) MAPSORT of: KIEE6515.seq  Check: 2373  from: 1  to: 459



KIEE6515.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     459 
   Size:         66     393
 
AfeI AGC'GCT

Cuts at:      0      87     459 
   Size:         87     372
 
AvaI C'yCGr_G

Cuts at:      0     140     287     459 
   Size:        140     147     172

  Fragments arranged by size: 

                 172     147     140
 
BanII G_rGCy'C

Cuts at:      0     352     459 
   Size:        352     107
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      19     459 
   Size:         19     440
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     149     302     459 
   Size:        149     153     157

  Fragments arranged by size: 

                 157     153     149
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     459 
   Size:        316     143
 
Bpu10I CC'TnA_GC

Cuts at:      0     119     448     459 
   Size:        119     329      11

  Fragments arranged by size: 

                 329     119      11
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     458     459 
   Size:        458       1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     340     343     459 
   Size:        340       3     116

  Fragments arranged by size: 

                 340     116       3
 
BseYI C'CCAG_C

Cuts at:      0     354     367     459 
   Size:        354      13      92

  Fragments arranged by size: 

                 354      92      13
 
BsiHKAI G_wGCw'C

Cuts at:      0     352     459 
   Size:        352     107
 
Bsp1286I G_dGCh'C

Cuts at:      0     352     459 
   Size:        352     107
 
BstYI r'GATC_y

Cuts at:      0     109     459 
   Size:        109     350
 
BtgI C'CryG_G

Cuts at:      0     191     459 
   Size:        191     268
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     386     459 
   Size:        386      73
 
BtsI GCAGTG_nn'

Cuts at:      0      56     341     459 
   Size:         56     285     118

  Fragments arranged by size: 

                 285     118      56
 
EaeI y'GGCC_r

Cuts at:      0       5     302     459 
   Size:          5     297     157

  Fragments arranged by size: 

                 297     157       5
 
EcoICRI GAG'CTC

Cuts at:      0     350     459 
   Size:        350     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     316     459 
   Size:         66     250     143

  Fragments arranged by size: 

                 250     143      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     145     459 
   Size:        145     314
 
HaeII r_GCGC'y

Cuts at:      0      89     295     459 
   Size:         89     206     164

  Fragments arranged by size: 

                 206     164      89
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       6      38     459 
   Size:          6      32     421

  Fragments arranged by size: 

                 421      32       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     459 
   Size:         87     372
 
MscI TGG'CCA

Cuts at:      0       7     304     459 
   Size:          7     297     155

  Fragments arranged by size: 

                 297     155       7
 
NcoI C'CATG_G

Cuts at:      0     191     459 
   Size:        191     268
 
PspXI vC'TCGA_Gb

Cuts at:      0     140     459 
   Size:        140     319
 
PstI C_TGCA'G

Cuts at:      0     445     459 
   Size:        445      14
 
SacI G_AGCT'C

Cuts at:      0     352     459 
   Size:        352     107
 
SfcI C'TryA_G

Cuts at:      0     180     441     459 
   Size:        180     261      18

  Fragments arranged by size: 

                 261     180      18
 
SmlI C'TyrA_G

Cuts at:      0     140     459 
   Size:        140     319
 
StyI C'CwwG_G

Cuts at:      0     191     459 
   Size:        191     268
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     289     459 
   Size:        289     170
 
XhoI C'TCGA_G

Cuts at:      0     140     459 
   Size:        140     319
 

 Enzymes that do cut:

     AcuI     AfeI     AvaI    BanII     BbsI     BplI     BpmI   Bpu10I
     BsaI    BseRI    BseYI  BsiHKAI Bsp1286I    BstYI     BtgI    BtgZI
     BtsI     EaeI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I     MmeI
     MscI     NcoI    PspXI     PstI     SacI     SfcI     SmlI     StyI
    TaqII     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI