################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflIII 81 ACGCGT A^C[GA][CT]GT AlwI 402 GATCC GGATC(4/5) Aor51HI 197 AGCGCT AGC^GCT BalI 395 TGGCCA TGG^CCA BanI 244 GGCACC G^G[CT][GA]CC BanII 448 GAGCCC G[GA]GC[CT]^C BcnI 223 CCGGG CC^[GC]GG BmyI 448 GAGCCC G[AGT]GC[ACT]^C BpmI 367 CTGGAG CTGGAG(16/14) Bsp1286I 448 GAGCCC G[AGT]GC[ACT]^C BspHI 281 TCATGA T^CATGA BsrI 257 ACTGG ACTGG(1/-1) BstEII 231 GGTCACC G^GT[ACGT]ACC BstPI 231 GGTCACC G^GT[ACGT]ACC BstYI 401 AGATCC [GA]^GATC[CT] DpnII 402 GATC ^GATC DraII 56 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 470 CCGTGG C^C[GA][CT]GG EaeI 395 TGGCCA [CT]^GGCC[GA] Eco47III 197 AGCGCT AGC^GCT EcoNI 48 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 56 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 231 GGTCACC G^GT[ACGT]ACC HapII 223 CCGG C^CGG HpaII 223 CCGG C^CGG MboI 402 GATC ^GATC MboII 314 TCTTC GAAGA(8/7) MflI 401 AGATCC [GA]^GATC[CT] MluI 81 ACGCGT A^CGCGT MluNI 395 TGGCCA TGG^CCA MscI 395 TGGCCA TGG^CCA MseI 479 TTAA T^TAA MspI 223 CCGG C^CGG NciI 223 CCGGG CC^[GC]GG NdeII 402 GATC ^GATC NspI 35 ACATGC [GA]CATG^[CT] PstI 136 CTGCAG CTGCA^G RcaI 281 TCATGA T^CATGA Sau3AI 402 GATC ^GATC SmlI 460 CTCAAG C^T[CT][GA]AG StuI 464 AGGCCT AGG^CCT TaqI 194 TCGA T^CGA Tru9I 479 TTAA T^TAA TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 194 TCGA T^CGA XhoII 401 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 82 CGCG CG^CG 103 CGCG AcyI 261 GACGCC G[GA]^CG[CT]C 361 GACGCC AfaI 156 GTAC GT^AC 417 GTAC AlwNI 55 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 292 CAGCTGCTG BsaHI 261 GACGCC G[GA]^CG[CT]C 361 GACGCC BstF5I 120 GGATG GGATG(2/0) 336 GGATG BstUI 82 CGCG CG^CG 103 CGCG FauI 451 CCCGC CCCGC(4/6) 104 GCGGG FokI 120 GGATG GGATG(9/13) 336 GGATG HgaI 261 GACGC GACGC(5/10) 361 GACGC Hin1I 261 GACGCC G[GA]^CG[CT]C 361 GACGCC HinfI 148 GAGTC G^A[ACGT]TC 331 GACTC MaeIII 232 GTCAC ^GT[ACGT]AC 427 GTCAC MvnI 82 CGCG CG^CG 103 CGCG PleI 331 GACTC GAGTC(4/5) 148 GAGTC PvuII 28 CAGCTG CAG^CTG 292 CAGCTG RsaI 156 GTAC GT^AC 417 GTAC SfaNI 72 GATGC GCATC(5/9) 337 GATGC SfcI 136 CTGCAG C^T[GA][CT]AG 187 CTACAG Tsp45I 232 GTCAC ^GT[GC]AC 427 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I CfoI Cfr13I EcoRII Fnu4HI HaeII HaeIII HhaI HinP1I HphI ItaI MnlI MspA1I MvaI MwoI NlaIII NlaIV PflMI Sau96I ScrFI TseI Van91I