Restriction map for
pFN21AE6512
(Circular) (Six-base) MAPSORT of: pFN21AE6512.seq Check: 8510 from: 1 to: 5194
pFN21AE6512 5194 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3004 278
Size: 53 83 186 936 1468 2468
Fragments arranged by size:
2468 1468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2537 1937
Size: 600 4594
Acc65I G'GTAC_C
Cuts at: 2515 2515
Size: 5194
AclI AA'CG_TT
Cuts at: 1563 3321 3694 1563
Size: 1758 373 3063
Fragments arranged by size:
3063 1758 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 3257 4641 4909 1632
Size: 488 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1137 488 268
AfeI AGC'GCT
Cuts at: 2204 2204
Size: 5194
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4997
AflIII A'CryG_T
Cuts at: 1203 2086 4093 5070 1203
Size: 883 2007 977 1327
Fragments arranged by size:
2007 1327 977 883
AgeI A'CCGG_T
Cuts at: 4025 4025
Size: 5194
AhdI GACnn_n'nnGTC
Cuts at: 3923 3923
Size: 5194
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5194
AlwNI CAG_nnn'CTG
Cuts at: 2065 2302 2937 4509 2065
Size: 237 635 1572 2750
Fragments arranged by size:
2750 1572 635 237
ApaLI G'TGCA_C
Cuts at: 3251 4407 3251
Size: 1156 4038
ApoI r'AATT_y
Cuts at: 1493 2503 2729 2881 4990 1493
Size: 1010 226 152 2109 1697
Fragments arranged by size:
2109 1697 1010 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3748 160
Size: 872 2716 1606
Fragments arranged by size:
2716 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5194
AvaI C'yCGr_G
Cuts at: 1870 1943 2519 1870
Size: 73 576 4545
Fragments arranged by size:
4545 576 73
BamHI G'GATC_C
Cuts at: 2524 2891 2524
Size: 367 4827
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2515 3970 618
Size: 325 217 627 462 266 1455 1842
Fragments arranged by size:
1842 1455 627 462 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2457 2513 1391
Size: 1066 56 4072
Fragments arranged by size:
4072 1066 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3385 3419 1903
Size: 34 1448 34 3678
Fragments arranged by size:
3678 1448 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3086 4296 3086
Size: 1210 3984
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5194
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3805 136
Size: 107 122 71 631 605 2133 1525
Fragments arranged by size:
2133 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5189 5189
Size: 5194
BlpI GC'TnA_GC
Cuts at: 2611 2611
Size: 5194
Bme1580I G_kGCm'C
Cuts at: 1790 3255 4411 1790
Size: 1465 1156 2573
Fragments arranged by size:
2573 1465 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5194
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2925 3883 442
Size: 416 53 83 399 24 1508 958 1753
Fragments arranged by size:
1753 1508 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 3854 1239
Size: 1154 1461 2579
Fragments arranged by size:
2579 1461 1154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 3319 4184 4482 2450
Size: 869 865 298 3162
Fragments arranged by size:
3162 869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3857 882
Size: 617 2358 2219
Fragments arranged by size:
2358 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2985 493
Size: 711 1781 2702
Fragments arranged by size:
2702 1781 711
BsaBI GATnn'nnATC
Cuts at: 5188 5188
Size: 5194
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2267 2367 3001 3383
Size: 53 83 186 936 734 100 634 382
Cuts at: 3383 4018 275
Size: 635 1451
Fragments arranged by size:
1451 936 734 635 634 382 186 100
83 53
BsaWI w'CCGG_w
Cuts at: 3627 4025 4299 4446 4857 3627
Size: 398 274 147 411 3964
Fragments arranged by size:
3964 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2144 2174 2375 2405 1178
Size: 30 165 30 741 30 201 30 3967
Fragments arranged by size:
3967 741 201 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5194
BseYI C'CCAG_C
Cuts at: 2178 4397 4893 2178
Size: 2219 496 2479
Fragments arranged by size:
2479 2219 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4621
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2644 3405 4433 1540
Size: 275 186 643 761 1028 2301
Fragments arranged by size:
2301 1028 761 643 275 186
BsiHKAI G_wGCw'C
Cuts at: 3255 3340 4411 3255
Size: 85 1071 4038
Fragments arranged by size:
4038 1071 85
BsmI GAATG_Cn'
Cuts at: 2705 2798 2705
Size: 93 5101
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2457 2513 3255 3340 4411 1391
Size: 399 667 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 667 399 85 56
BspEI T'CCGG_A
Cuts at: 4857 4857
Size: 5194
BspHI T'CATG_A
Cuts at: 2286 3083 4813 2286
Size: 797 1730 2667
Fragments arranged by size:
2667 1730 797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2549 844
Size: 1705 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3081 1394
Size: 429 1258 3507
Fragments arranged by size:
3507 1258 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3689 3863 65
Size: 1183 2441 174 1396
Fragments arranged by size:
2441 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3838 4025 1700
Size: 110 2028 187 2869
Fragments arranged by size:
2869 2028 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5194
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5194
BssSI C'ACGA_G
Cuts at: 3254 4266 3254
Size: 1012 4182
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5194
BstBI TT'CG_AA
Cuts at: 3998 3998
Size: 5194
BstEII G'GTnAC_C
Cuts at: 1184 2236 1184
Size: 1052 4142
BstYI r'GATC_y
Cuts at: 1972 2406 2524 2891 3276 3293 4745 4831 4843
Size: 434 118 367 385 17 1452 86 12
Cuts at: 4843 5189 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 434 385 367 346 118 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2475 513
Size: 552 939 471 3232
Fragments arranged by size:
3232 939 552 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4455
Fragments arranged by size:
4455 683 56
BtsI GCAGTG_nn'
Cuts at: 2310 2710 3504 3524 2310
Size: 400 794 20 3980
Fragments arranged by size:
3980 794 400 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5194
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5159
DraI TTT'AAA
Cuts at: 2498 2498
Size: 5194
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4201 809
Size: 507 2885 1802
Fragments arranged by size:
2885 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2641 3530
Size: 54 1425 50 275 18 570 241 889
Cuts at: 3530 5159 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 570 275 241 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2641 1537
Size: 275 829 4090
Fragments arranged by size:
4090 829 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3124 1353
Size: 1771 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3781 4155 4301 387
Size: 1064 138 435 1757 374 146 1280
Fragments arranged by size:
1757 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2393 3257 3854 4641 4909 1239
Size: 393 488 273 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 597 488 393 273 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2057
Size: 5194
EcoO109I rG'GnC_Cy
Cuts at: 2062 2062
Size: 5194
EcoRI G'AATT_C
Cuts at: 2503 2881 2503
Size: 378 4816
FspI TGC'GCA
Cuts at: 3700 3700
Size: 5194
HaeII r_GCGC'y
Cuts at: 1678 2122 2206 2389 4341 1678
Size: 444 84 183 1952 2531
Fragments arranged by size:
2531 1952 444 183 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 3835 3867 3909
Size: 32 135 32 803 32 1428 32 42
Cuts at: 3909 3941 1373
Size: 32 2626
Fragments arranged by size:
2626 1428 803 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2538 2784 669
Size: 988 281 600 246 3079
Fragments arranged by size:
3079 988 600 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5194
HpaI GTT'AAC
Cuts at: 2784 2784
Size: 5194
KpnI G_GTAC'C
Cuts at: 2519 2519
Size: 5194
MfeI C'AATT_G
Cuts at: 2793 2793
Size: 5194
MluI A'CGCG_T
Cuts at: 2086 5070 2086
Size: 2984 2210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2373 4308 4492 1305
Size: 1068 1935 184 2007
Fragments arranged by size:
2007 1935 1068 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2402 10
Size: 54 1425 343 570 2802
Fragments arranged by size:
2802 1425 570 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3152 3511 3670 518
Size: 648 1986 359 159 2042
Fragments arranged by size:
2042 1986 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2047 2150 2188 2299 2610 3287 4435
Size: 429 12 103 38 111 311 677 1148
Cuts at: 4435 4680 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 429 311 245 111 103
38 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3703
Fragments arranged by size:
3703 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5194
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5194
NotI GC'GGCC_GC
Cuts at: 2641 2641
Size: 5194
NspI r_CATG'y
Cuts at: 1132 1229 2044 2552 4097 1132
Size: 97 815 508 1545 2229
Fragments arranged by size:
2229 1545 815 508 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5194
PciI A'CATG_T
Cuts at: 4093 4093
Size: 5194
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 2322 2352 1108
Size: 1194 20 30 3950
Fragments arranged by size:
3950 1194 30 20
PfoI T'CCnGG_A
Cuts at: 2052 2052
Size: 5194
PmeI GTTT'AAAC
Cuts at: 2498 2498
Size: 5194
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 4776 4808 3263
Size: 32 1481 32 3649
Fragments arranged by size:
3649 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5194
PsiI TTA'TAA
Cuts at: 2764 2764
Size: 5194
PstI C_TGCA'G
Cuts at: 830 2145 2546 830
Size: 1315 401 3478
Fragments arranged by size:
3478 1315 401
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5194
PvuII CAG'CTG
Cuts at: 2035 2299 2035
Size: 264 4930
SalI G'TCGA_C
Cuts at: 1936 2536 1936
Size: 600 4594
SbfI CC_TGCA'GG
Cuts at: 2546 2546
Size: 5194
ScaI AGT'ACT
Cuts at: 3442 5154 3442
Size: 1712 3482
SfcI C'TryA_G
Cuts at: 826 1045 2141 2192 2542 3677 4358 4549 826
Size: 219 1096 51 350 1135 681 191 1471
Fragments arranged by size:
1471 1135 1096 681 350 219 191 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5194
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5194
SmaI CCC'GGG
Cuts at: 1872 2521 1872
Size: 649 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 3298 4199 4461 820
Size: 197 926 522 833 901 262 1553
Fragments arranged by size:
1553 926 901 833 522 262 197
SnaBI TAC'GTA
Cuts at: 493 2985 493
Size: 2492 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5194
SphI G_CATG'C
Cuts at: 1132 2552 1132
Size: 1420 3774
SspI AAT'ATT
Cuts at: 5 52 3118 5
Size: 47 3066 2081
Fragments arranged by size:
3066 2081 47
StuI AGG'CCT
Cuts at: 1517 2471 1517
Size: 954 4240
StyI C'CwwG_G
Cuts at: 513 1065 2004 2633 513
Size: 552 939 629 3074
Fragments arranged by size:
3074 939 629 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 3221 3238 3391 3576 4021 325
Size: 1255 612 1029 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1029 612 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 3440 5152
Size: 274 80 33 51 1443 444 1019 1712
Cuts at: 5152 96
Size: 138
Fragments arranged by size:
1712 1443 1019 444 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5154 5186 5154
Size: 32 5162
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5194
XbaI T'CTAG_A
Cuts at: 2530 2530
Size: 5194
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5194
XmaI C'CCGG_G
Cuts at: 1870 2519 1870
Size: 649 4545
XmnI GAAnn'nnTTC
Cuts at: 3323 3323
Size: 5194
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3002 276
Size: 53 83 186 936 1468 2468
Fragments arranged by size:
2468 1468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI