(Linear) (Six-base) MAPSORT of: KIEE6512.seq Check: 6225 from: 1 to: 489 KIEE6512.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 489 Size: 115 374 AfeI AGC'GCT Cuts at: 0 199 489 Size: 199 290 AflIII A'CryG_T Cuts at: 0 81 489 Size: 81 408 AlwNI CAG_nnn'CTG Cuts at: 0 60 297 489 Size: 60 237 192 Fragments arranged by size: 237 192 60 BanI G'GyrC_C Cuts at: 0 244 489 Size: 244 245 BanII G_rGCy'C Cuts at: 0 452 489 Size: 452 37 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 489 Size: 388 101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 489 Size: 445 44 BsaHI Gr'CG_yC Cuts at: 0 262 362 489 Size: 262 100 127 Fragments arranged by size: 262 127 100 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 139 169 370 400 489 Size: 139 30 201 30 89 Fragments arranged by size: 201 139 89 30 30 BseYI C'CCAG_C Cuts at: 0 173 489 Size: 173 316 Bsp1286I G_dGCh'C Cuts at: 0 452 489 Size: 452 37 BspHI T'CATG_A Cuts at: 0 281 489 Size: 281 208 BstEII G'GTnAC_C Cuts at: 0 231 489 Size: 231 258 BstYI r'GATC_y Cuts at: 0 401 489 Size: 401 88 BtgI C'CryG_G Cuts at: 0 470 489 Size: 470 19 BtsI GCAGTG_nn' Cuts at: 0 305 489 Size: 305 184 EaeI y'GGCC_r Cuts at: 0 395 489 Size: 395 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 489 Size: 19 470 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 388 489 Size: 115 273 101 Fragments arranged by size: 273 115 101 EcoNI CCTnn'n_nnAGG Cuts at: 0 52 489 Size: 52 437 EcoO109I rG'GnC_Cy Cuts at: 0 57 489 Size: 57 432 HaeII r_GCGC'y Cuts at: 0 117 201 384 489 Size: 117 84 183 105 Fragments arranged by size: 183 117 105 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 370 402 489 Size: 370 32 87 Fragments arranged by size: 370 87 32 MluI A'CGCG_T Cuts at: 0 81 489 Size: 81 408 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 368 489 Size: 368 121 MscI TGG'CCA Cuts at: 0 397 489 Size: 397 92 MspA1I CmG'CkG Cuts at: 0 30 42 145 183 294 489 Size: 30 12 103 38 111 195 Fragments arranged by size: 195 111 103 38 30 12 NspI r_CATG'y Cuts at: 0 39 489 Size: 39 450 PflMI CCAn_nnn'nTGG Cuts at: 0 297 317 347 489 Size: 297 20 30 142 Fragments arranged by size: 297 142 30 20 PfoI T'CCnGG_A Cuts at: 0 47 489 Size: 47 442 PstI C_TGCA'G Cuts at: 0 140 489 Size: 140 349 PvuII CAG'CTG Cuts at: 0 30 294 489 Size: 30 264 195 Fragments arranged by size: 264 195 30 SfcI C'TryA_G Cuts at: 0 136 187 489 Size: 136 51 302 Fragments arranged by size: 302 136 51 SmlI C'TyrA_G Cuts at: 0 460 489 Size: 460 29 StuI AGG'CCT Cuts at: 0 466 489 Size: 466 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 187 489 Size: 187 302 TatI w'GTAC_w Cuts at: 0 416 489 Size: 416 73 Enzymes that do cut: AcuI AfeI AflIII AlwNI BanI BanII BpmI BpuEI BsaHI BsaXI BseYI Bsp1286I BspHI BstEII BstYI BtgI BtsI EaeI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I MluI MmeI MscI MspA1I NspI PflMI PfoI PstI PvuII SfcI SmlI StuI TaqII TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI