################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AatII 858 GACGTC GACGT^C AcyI 858 GACGTC G[GA]^CG[CT]C AvaII 826 GGACC G^G[AT]CC BglII 135 AGATCT A^GATCT BsaAI 982 TACGTA [CT]AC^GT[GA] BsaHI 858 GACGTC G[GA]^CG[CT]C BsgI 206 CTGCAC GTGCAG(16/14) BsmI 485 GAATGC GAATGC(1/-1) BspHI 1077 TCATGA T^CATGA BstYI 135 AGATCT [GA]^GATC[CT] Cfr13I 826 GGACC G^G[ACGT]CC DrdI 858 GACGTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1025 CCACGG C^C[GA][CT]GG EarI 17 CTCTTC CTCTTC(1/4) EcoT14I 245 CCAAGG C^C[AT][AT]GG Hin1I 858 GACGTC G[GA]^CG[CT]C HindIII 113 AAGCTT A^AGCTT HphI 376 GGTGA GGTGA(8/7) Ksp632I 17 CTCTTC CTCTTC(1/4) MflI 135 AGATCT [GA]^GATC[CT] NdeI 104 CATATG CA^TATG NlaIII 1078 CATG CATG^ NlaIV 1129 GGTTCC GG[ACGT]^[ACGT]CC RcaI 1077 TCATGA T^CATGA Sau96I 826 GGACC G^G[ACGT]CC SfaNI 258 GATGC GCATC(5/9) SnaBI 982 TACGTA TAC^GTA StuI 734 AGGCCT AGG^CCT StyI 245 CCAAGG C^C[AT][AT]GG TfiI 501 GAATC G^A[AT]TC Tsp509I 78 AATT ^AATT XhoII 135 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 337 GTAGAC GT^[AC][GT]AC 1015 GTAGAC AlwNI 679 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 764 CAGCTTCTG Asp700I 13 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 612 GAATGACTTC BfaI 238 CTAG C^TAG 667 CTAG BpmI 917 CTCCAG CTGGAG(16/14) 1071 CTCCAG BsmAI 691 GAGAC GTCTC(1/5) 729 GTCTC BsmFI 531 GGGAC GGGAC(10/14) 525 GTCCC Bsp1407I 108 TGTACA T^GTACA 1170 TGTACA BsrGI 108 TGTACA T^GTACA 1170 TGTACA Cac8I 208 GCACGC GC[ACGT]^[ACGT]GC 698 GCCAGC MaeI 238 CTAG C^TAG 667 CTAG MaeIII 154 GTAAC ^GT[ACGT]AC 980 GTTAC MspA1I 520 CAGCTG C[AC]G^C[GT]G 682 CAGCTG PshAI 805 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1069 GACTCCAGTC PstI 673 CTGCAG CTGCA^G 807 CTGCAG PvuII 520 CAGCTG CAG^CTG 682 CAGCTG SspBI 108 TGTACA T^GTACA 1170 TGTACA XcmI 852 CCAGAGGACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1137 CCACGTTCAGAATGG XmnI 13 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 612 GAATGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI BbvI BsaJI BseRI BsiYI BslI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HinfI ItaI MaeII MboI MboII MnlI MseI MvaI MwoI NdeII PflMI PleI RsaI Sau3AI ScrFI SfcI SmlI TaqI Tru9I TseI TspRI TthHB8I Van91I