(Circular) (Six-base) MAPSORT of: pFN21AE9641.seq Check: 4825 from: 1 to: 5893 pFN21AE9641 5893 bp With 182 enzymes: SgfI * March 28, 2012 12:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2867 3703 278 Size: 53 83 186 936 1331 836 2468 Fragments arranged by size: 2468 1331 936 836 186 83 53 AccI GT'mk_AC Cuts at: 1937 2343 3021 3236 1937 Size: 406 678 215 4594 Fragments arranged by size: 4594 678 406 215 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 2711 3956 5340 5608 1632 Size: 567 512 1245 1384 268 1917 Fragments arranged by size: 1917 1384 1245 567 512 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 4792 5769 1203 Size: 3589 977 1327 Fragments arranged by size: 3589 1327 977 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5893 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2226 1184 Size: 1042 4851 AlwNI CAG_nnn'CTG Cuts at: 2689 2774 3636 5208 2689 Size: 85 862 1572 3374 Fragments arranged by size: 3374 1572 862 85 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 3202 3428 3580 5689 1493 Size: 1709 226 152 2109 1697 Fragments arranged by size: 2109 1709 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 3218 1870 Size: 73 1275 4545 Fragments arranged by size: 4545 1275 73 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3214 4669 618 Size: 325 217 627 1427 1455 1842 Fragments arranged by size: 1842 1455 1427 627 325 217 BanII G_rGCy'C Cuts at: 1391 3212 1391 Size: 1821 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4922 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4084 4118 1903 Size: 34 2147 34 3678 Fragments arranged by size: 3678 2147 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4504 136 Size: 107 122 71 631 605 2832 1525 Fragments arranged by size: 2832 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2140 5888 2140 Size: 3748 2145 BlpI GC'TnA_GC Cuts at: 3310 3310 Size: 5893 Bme1580I G_kGCm'C Cuts at: 1790 3954 5110 1790 Size: 2164 1156 2573 Fragments arranged by size: 2573 2164 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3624 4582 442 Size: 416 53 83 399 24 2207 958 1753 Fragments arranged by size: 2207 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2907 3061 4553 1239 Size: 1668 154 1492 2579 Fragments arranged by size: 2579 1668 1492 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2279 3075 3148 4018 4883 5181 2279 Size: 796 73 870 865 298 2991 Fragments arranged by size: 2991 870 865 796 298 73 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4556 882 Size: 617 3057 2219 Fragments arranged by size: 3057 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2989 3684 493 Size: 711 1785 695 2702 Fragments arranged by size: 2702 1785 711 695 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2864 3700 4082 4717 Size: 53 83 186 936 1331 836 382 635 Cuts at: 4717 275 Size: 1451 Fragments arranged by size: 1451 1331 936 836 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4326 4724 4998 5145 5556 4326 Size: 398 274 147 411 4663 Fragments arranged by size: 4663 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 3059 3089 1178 Size: 30 165 30 714 30 912 30 3982 Fragments arranged by size: 3982 912 714 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 2839 2972 2988 3110 1186 Size: 1417 236 133 16 122 3969 Fragments arranged by size: 3969 1417 236 133 122 16 BseYI C'CCAG_C Cuts at: 2347 5096 5592 2347 Size: 2749 496 2648 Fragments arranged by size: 2749 2648 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2196 1163 Size: 573 460 4860 Fragments arranged by size: 4860 573 460 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3954 4039 5110 3954 Size: 85 1071 4737 Fragments arranged by size: 4737 1071 85 BsmI GAATG_Cn' Cuts at: 2496 3404 3497 2496 Size: 908 93 4892 Fragments arranged by size: 4892 908 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3212 3954 4039 5110 1391 Size: 399 1422 742 85 1071 2174 Fragments arranged by size: 2174 1422 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5556 5556 Size: 5893 BspHI T'CATG_A Cuts at: 3082 3782 5512 3082 Size: 700 1730 3463 Fragments arranged by size: 3463 1730 700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3248 844 Size: 2404 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4388 4562 65 Size: 1183 3140 174 1396 Fragments arranged by size: 3140 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4537 4724 1700 Size: 110 2727 187 2869 Fragments arranged by size: 2869 2727 187 110 BsrGI T'GTAC_A Cuts at: 96 2113 3175 96 Size: 2017 1062 2814 Fragments arranged by size: 2814 2017 1062 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstYI r'GATC_y Cuts at: 1972 2140 3223 3590 3975 3992 5444 5530 5542 Size: 168 1083 367 385 17 1452 86 12 Cuts at: 5542 5888 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1083 385 367 346 168 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3030 513 Size: 552 1965 3376 Fragments arranged by size: 3376 1965 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 3043 3111 3409 4203 4223 3043 Size: 68 298 794 20 4713 Fragments arranged by size: 4713 794 298 68 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2869 4900 809 Size: 507 1553 2031 1802 Fragments arranged by size: 2031 1802 1553 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858 Size: 54 1425 50 275 18 1510 889 1629 Cuts at: 5858 8 Size: 43 Fragments arranged by size: 1629 1510 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 3823 1353 Size: 675 1795 3423 Fragments arranged by size: 3423 1795 675 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2711 2907 3061 3956 4553 5340 Size: 393 567 512 196 154 895 597 787 Cuts at: 5340 5608 1239 Size: 268 1524 Fragments arranged by size: 1524 895 787 597 567 512 393 268 196 154 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2039 2039 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 3059 3091 4534 Size: 32 135 32 560 32 895 32 1443 Cuts at: 4534 4566 4608 4640 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 895 560 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3237 3483 669 Size: 988 281 1299 246 3079 Fragments arranged by size: 3079 1299 988 281 246 HindIII A'AGCT_T Cuts at: 748 2118 748 Size: 1370 4523 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2970 5007 5191 1305 Size: 1665 2037 184 2007 Fragments arranged by size: 2037 2007 1665 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3851 4210 4369 518 Size: 648 2685 359 159 2042 Fragments arranged by size: 2685 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2527 2689 3309 3986 5134 5379 1606 Size: 921 162 620 677 1148 245 2120 Fragments arranged by size: 2120 1148 921 677 620 245 162 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5341 NdeI CA'TA_TG Cuts at: 387 2110 387 Size: 1723 4170 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 3251 4796 1132 Size: 97 2022 1545 2229 Fragments arranged by size: 2229 2022 1545 97 PasI CC'CwG_GG Cuts at: 1348 2533 1348 Size: 1185 4708 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 2710 2840 2930 1108 Size: 1602 130 90 4071 Fragments arranged by size: 4071 1602 130 90 PfoI T'CCnGG_A Cuts at: 2499 2499 Size: 5893 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2814 3078 1613 Size: 1201 264 4428 Fragments arranged by size: 4428 1201 264 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PstI C_TGCA'G Cuts at: 830 2682 2816 3245 830 Size: 1852 134 429 3478 Fragments arranged by size: 3478 1852 429 134 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 PvuII CAG'CTG Cuts at: 2527 2689 2527 Size: 162 5731 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SfcI C'TryA_G Cuts at: 826 1045 2678 2766 2781 2812 3241 4376 5057 Size: 219 1633 88 15 31 429 1135 681 Cuts at: 5057 5248 826 Size: 191 1471 Fragments arranged by size: 1633 1471 1135 681 429 219 191 88 31 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2294 3090 3163 3997 4898 5160 Size: 197 926 351 796 73 834 901 262 Cuts at: 5160 820 Size: 1553 Fragments arranged by size: 1553 926 901 834 796 351 262 197 73 SnaBI TAC'GTA Cuts at: 493 2989 3684 493 Size: 2496 695 2702 Fragments arranged by size: 2702 2496 695 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 2741 1517 Size: 1224 4669 StyI C'CwwG_G Cuts at: 513 1065 2250 3332 513 Size: 552 1185 1082 3074 Fragments arranged by size: 3074 1185 1082 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3920 3937 4090 4275 4720 325 Size: 1255 2340 17 153 185 445 1498 Fragments arranged by size: 2340 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2113 2133 2273 Size: 274 80 33 51 1443 136 20 140 Cuts at: 2273 3175 4139 5851 96 Size: 902 964 1712 138 Fragments arranged by size: 1712 1443 964 902 274 140 138 136 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5853 5885 5853 Size: 32 5861 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2864 3149 2864 Size: 285 5608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 2022 2621 4022 2022 Size: 599 1401 3893 Fragments arranged by size: 3893 1401 599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2865 3701 276 Size: 53 83 186 936 1331 836 2468 Fragments arranged by size: 2468 1331 936 836 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI