################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tsp45I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AfaI 592 GTAC GT^AC AlwI 153 GATCC GGATC(4/5) AspI 827 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 171 GGACC G^G[AT]CC BalI 575 TGGCCA TGG^CCA BclI 308 TGATCA T^GATCA BcnI 36 CCGGG CC^[GC]GG BpmI 80 CTGGAG CTGGAG(16/14) BsmAI 98 GAGAC GTCTC(1/5) Bsp1407I 591 TGTACA T^GTACA BsrGI 591 TGTACA T^GTACA BstF5I 421 GGATG GGATG(2/0) BstXI 614 CCAAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 152 AGATCC [GA]^GATC[CT] Cfr13I 171 GGACC G^G[ACGT]CC DsaI 13 CCGTGG C^C[GA][CT]GG EaeI 575 TGGCCA [CT]^GGCC[GA] EarI 6 CTCTTC CTCTTC(1/4) EcoRI 90 GAATTC G^AATTC EcoT14I 252 CCTTGG C^C[AT][AT]GG FbaI 308 TGATCA T^GATCA FokI 421 GGATG GGATG(9/13) HaeII 940 GGCGCT [GA]GCGC^[CT] HapII 36 CCGG C^CGG HincII 298 GTTGAC GT[CT]^[GA]AC HindII 298 GTTGAC GT[CT]^[GA]AC HinfI 827 GACTC G^A[ACGT]TC HpaII 36 CCGG C^CGG HphI 333 TCACC GGTGA(8/7) Ksp632I 6 CTCTTC CTCTTC(1/4) MaeII 767 ACGT A^CGT MflI 152 AGATCC [GA]^GATC[CT] MluNI 575 TGGCCA TGG^CCA MscI 575 TGGCCA TGG^CCA MspI 36 CCGG C^CGG NciI 36 CCGGG CC^[GC]GG NlaIV 18 GGAGCC GG[ACGT]^[ACGT]CC PflFI 827 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 827 GACTC GAGTC(4/5) PstI 560 CTGCAG CTGCA^G RsaI 592 GTAC GT^AC Sau96I 171 GGACC G^G[ACGT]CC SspBI 591 TGTACA T^GTACA StyI 252 CCTTGG C^C[AT][AT]GG Tth111I 827 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 174 CCAGGCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 152 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BfaI 905 CTAG C^TAG 937 CTAG BsaJI 13 CCGTGG C^C[ACGT][ACGT]GG 252 CCTTGG BseRI 344 CTCCTC GAGGAG(10/8) 866 GAGGAG BstNI 79 CCTGG CC^[AT]GG 174 CCAGG CfoI 33 GCGC GCG^C 941 GCGC DdeI 86 CTGAG C^T[ACGT]AG 347 CTCAG DpnII 153 GATC ^GATC 309 GATC EcoRII 79 CCTGG ^CC[AT]GG 174 CCAGG HhaI 33 GCGC GCG^C 941 GCGC HinP1I 33 GCGC G^CGC 941 GCGC MaeI 905 CTAG C^TAG 937 CTAG MboI 153 GATC ^GATC 309 GATC MseI 446 TTAA T^TAA 686 TTAA MvaI 79 CCTGG CC^[AT]GG 174 CCAGG NdeI 376 CATATG CA^TATG 878 CATATG NdeII 153 GATC ^GATC 309 GATC NspI 178 GCATGT [GA]CATG^[CT] 294 GCATGT Sau3AI 153 GATC ^GATC 309 GATC SfaNI 145 GATGC GCATC(5/9) 499 GCATC SfcI 560 CTGCAG C^T[GA][CT]AG 944 CTACAG Tru9I 446 TTAA T^TAA 686 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AcsI AluI ApoI BbvI BsiYI BslI BsmFI BsrI BssKI Cac8I Fnu4HI HaeIII ItaI MboII MnlI MwoI NlaIII ScrFI TseI Tsp509I TspRI