Restriction map for
pFN21AE3992
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanII BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I BspDI BspEI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstUI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KspI MaeII MamI MfeI MflI
MluI MroI MspA1I MunI MvnI
NarI NcoI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspI
StuI StyI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 350 GGTACC G^GTACC
AciI 819 CCGC CCGC(-3/-1)
AlwNI 992 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 350 GGTACC G^GTACC
AvaII 1149 GGACC G^G[AT]CC
BalI 680 TGGCCA TGG^CCA
BbvCI 1045 GCTGAGG CCTCAGC(-5/-2)
BcnI 25 CCCGG CC^[GC]GG
BpmI 1017 CTCCAG CTGGAG(16/14)
BsaI 4 GAGACC GGTCTC(1/5)
BseRI 407 CTCCTC GAGGAG(10/8)
Bsp1407I 521 TGTACA T^GTACA
BspHI 411 TCATGA T^CATGA
BsrDI 1075 CATTGC GCAATG(2/0)
BsrFI 777 GCCGGC [GA]^CCGG[CT]
BsrGI 521 TGTACA T^GTACA
BstAPI 879 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 494 TTCGAA TT^CGAA
BstEII 856 GGTGACC G^GT[ACGT]ACC
BstPI 856 GGTGACC G^GT[ACGT]ACC
BstXI 8 CCAAACGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 777 GCCGGC [GA]^CCGG[CT]
Cfr13I 1149 GGACC G^G[ACGT]CC
DpnII 43 GATC ^GATC
DraI 930 TTTAAA TTT^AAA
EaeI 680 TGGCCA [CT]^GGCC[GA]
EarI 572 GAAGAG CTCTTC(1/4)
EcoO65I 856 GGTGACC G^GT[ACGT]ACC
EcoRI 536 GAATTC G^AATTC
EcoRV 454 GATATC GAT^ATC
HaeIII 681 GGCC GG^CC
HgaI 32 GCGTC GACGC(5/10)
KpnI 350 GGTACC GGTAC^C
Ksp632I 572 GAAGAG CTCTTC(1/4)
MboI 43 GATC ^GATC
MluNI 680 TGGCCA TGG^CCA
MscI 680 TGGCCA TGG^CCA
NaeI 777 GCCGGC GCC^GGC
NciI 25 CCCGG CC^[GC]GG
NdeI 296 CATATG CA^TATG
NdeII 43 GATC ^GATC
NgoMIV 777 GCCGGC G^CCGGC
NspV 494 TTCGAA TT^CGAA
PflMI 292 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 804 GAGTC GAGTC(4/5)
RcaI 411 TCATGA T^CATGA
Sau3AI 43 GATC ^GATC
Sau96I 1149 GGACC G^G[ACGT]CC
SfaNI 994 GATGC GCATC(5/9)
SfuI 494 TTCGAA TT^CGAA
SmlI 233 CTCAAG C^T[CT][GA]AG
SpeI 339 ACTAGT A^CTAGT
SspBI 521 TGTACA T^GTACA
Van91I 292 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanI 350 GGTACC G^G[CT][GA]CC
660 GGCACC
BbsI 1024 GAAGAC GAAGAC(2/6)
205 GTCTTC
BpuAI 1024 GAAGAC GAAGAC(2/6)
205 GTCTTC
BsaJI 964 CCACGG C^C[ACGT][ACGT]GG
1058 CCGTGG
BsiYI 172 CCTCAGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
292 CCAACATATGG
BslI 172 CCTCAGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
292 CCAACATATGG
BsmFI 707 GGGAC GGGAC(10/14)
1050 GGGAC
DsaI 964 CCACGG C^C[GA][CT]GG
1058 CCGTGG
HapII 26 CCGG C^CGG
778 CCGG
HpaII 26 CCGG C^CGG
778 CCGG
HphI 238 GGTGA GGTGA(8/7)
856 GGTGA
MaeIII 746 GTGAC ^GT[ACGT]AC
857 GTGAC
MslI 412 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
622 CATAGAAATG
MspI 26 CCGG C^CGG
778 CCGG
MwoI 879 GCAACCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
888 GCAGCAAAAGC
NlaIV 350 GGTACC GG[ACGT]^[ACGT]CC
660 GGCACC
PstI 48 CTGCAG CTGCA^G
886 CTGCAG
SfcI 48 CTGCAG C^T[GA][CT]AG
886 CTGCAG
Tsp45I 746 GTGAC ^GT[GC]AC
857 GTGAC
XcmI 647 CCAAACTTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
700 CCATTAAGGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsmAI BsrI BssKI BstF5I
BstNI Cac8I DdeI EcoRII Fnu4HI
FokI HinfI ItaI MaeI MboII
MnlI MseI MvaI NlaIII NspI
RsaI ScrFI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I