Restriction map for
pFN21AE5322
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 85 GTAGAC GT^[AC][GT]AC
AccII 184 CGCG CG^CG
AcyI 292 GGCGTC G[GA]^CG[CT]C
AfaI 253 GTAC GT^AC
AflIII 155 ACGTGT A^C[GA][CT]GT
AspI 175 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 323 TGGCCA TGG^CCA
BamHI 557 GGATCC G^GATCC
BlpI 207 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 207 GCTCAGC GC^T[ACGT]AGC
BsaHI 292 GGCGTC G[GA]^CG[CT]C
BsaI 113 GAGACC GGTCTC(1/5)
BsgI 367 CTGCAC GTGCAG(16/14)
BsiEI 465 CGACCG CG[GA][CT]^CG
BstUI 184 CGCG CG^CG
CelII 207 GCTCAGC GC^T[ACGT]AGC
DsaI 494 CCGTGG C^C[GA][CT]GG
EaeI 323 TGGCCA [CT]^GGCC[GA]
EcoNI 366 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 293 GCGTC GACGC(5/10)
Hin1I 292 GGCGTC G[GA]^CG[CT]C
HincII 151 GTTGAC GT[CT]^[GA]AC
HindII 151 GTTGAC GT[CT]^[GA]AC
HphI 703 GGTGA GGTGA(8/7)
MaeIII 42 GTCAC ^GT[ACGT]AC
MboII 378 GAAGA GAAGA(8/7)
MluNI 323 TGGCCA TGG^CCA
MscI 323 TGGCCA TGG^CCA
MslI 489 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 521 CCGCTG C[AC]G^C[GT]G
MvnI 184 CGCG CG^CG
NlaIII 671 CATG CATG^
NspI 670 GCATGC [GA]CATG^[CT]
PflFI 175 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 227 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 253 GTAC GT^AC
SacI 220 GAGCTC GAGCT^C
SmaI 625 CCCGGG CCC^GGG
SphI 670 GCATGC GCATG^C
Tsp45I 42 GTCAC ^GT[GC]AC
Tth111I 175 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 261 CCACCGAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 625 CCCGGG C^CCGGG
XmaI 625 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 554 TCCGGA T^CCGGA
688 TCCGGA
AocI 453 CCTGAGG CC^T[ACGT]AGG
698 CCTCAGG
AvaII 228 GGTCC G^G[AT]CC
723 GGACC
BglI 4 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
284 GCCACCTTGGC
BsaWI 554 TCCGGA [AT]^CCGG[AT]
688 TCCGGA
BseAI 554 TCCGGA T^CCGGA
688 TCCGGA
BspEI 554 TCCGGA T^CCGGA
688 TCCGGA
BsrI 785 ACTGG ACTGG(1/-1)
664 CCAGT
BstYI 380 AGATCC [GA]^GATC[CT]
557 GGATCC
Bsu36I 453 CCTGAGG CC^T[ACGT]AGG
698 CCTCAGG
DpnII 381 GATC ^GATC
558 GATC
Eco81I 453 CCTGAGG CC^T[ACGT]AGG
698 CCTCAGG
EcoT14I 288 CCTTGG C^C[AT][AT]GG
751 CCAAGG
MaeII 80 ACGT A^CGT
155 ACGT
MboI 381 GATC ^GATC
558 GATC
MflI 380 AGATCC [GA]^GATC[CT]
557 GGATCC
MroI 554 TCCGGA T^CCGGA
688 TCCGGA
NdeII 381 GATC ^GATC
558 GATC
PaeR7I 223 CTCGAG C^TCGAG
398 CTCGAG
Sau3AI 381 GATC ^GATC
558 GATC
SmlI 223 CTCGAG C^T[CT][GA]AG
398 CTCGAG
StyI 288 CCTTGG C^C[AT][AT]GG
751 CCAAGG
TaqI 224 TCGA T^CGA
399 TCGA
TthHB8I 224 TCGA T^CGA
399 TCGA
XhoI 223 CTCGAG C^TCGAG
398 CTCGAG
XhoII 380 AGATCC [GA]^GATC[CT]
557 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MnlI MspI MvaI MwoI NciI
NlaIV PleI PstI Sau96I ScrFI
SfcI TseI TspRI