(Circular) (Six-base) MAPSORT of: pFN21AE5322.seq Check: 1259 from: 1 to: 5497 pFN21AE5322 5497 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2335 2335 Size: 5497 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3307 278 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2091 2840 1937 Size: 154 749 4594 Fragments arranged by size: 4594 749 154 Acc65I G'GTAC_C Cuts at: 2818 2818 Size: 5497 AclI AA'CG_TT Cuts at: 1563 3624 3997 1563 Size: 2061 373 3063 Fragments arranged by size: 3063 2061 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 3560 4944 5212 1632 Size: 464 1464 1384 268 1917 Fragments arranged by size: 1917 1464 1384 464 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5300 AflIII A'CryG_T Cuts at: 1203 2160 4396 5373 1203 Size: 957 2236 977 1327 Fragments arranged by size: 2236 1327 977 957 AgeI A'CCGG_T Cuts at: 4328 4328 Size: 5497 AhdI GACnn_n'nnGTC Cuts at: 4226 4226 Size: 5497 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5497 AlwNI CAG_nnn'CTG Cuts at: 3240 4812 3240 Size: 1572 3925 ApaLI G'TGCA_C Cuts at: 3554 4710 3554 Size: 1156 4341 ApoI r'AATT_y Cuts at: 1493 2806 3032 3184 5293 1493 Size: 1313 226 152 2109 1697 Fragments arranged by size: 2109 1697 1313 226 152 AseI AT'TA_AT Cuts at: 160 1032 4051 160 Size: 872 3019 1606 Fragments arranged by size: 3019 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 AvaI C'yCGr_G Cuts at: 1870 1943 2131 2228 2403 2598 2630 2822 1870 Size: 73 188 97 175 195 32 192 4545 Fragments arranged by size: 4545 195 192 188 175 97 73 32 BamHI G'GATC_C Cuts at: 2562 2827 3194 2562 Size: 265 367 4865 Fragments arranged by size: 4865 367 265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2818 4273 618 Size: 325 217 627 1031 1455 1842 Fragments arranged by size: 1842 1455 1031 627 325 217 BanII G_rGCy'C Cuts at: 1391 2229 2574 2654 2816 1391 Size: 838 345 80 162 4072 Fragments arranged by size: 4072 838 345 162 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3688 3722 1903 Size: 34 1751 34 3678 Fragments arranged by size: 3678 1751 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3389 4599 3389 Size: 1210 4287 BclI T'GATC_A Cuts at: 1548 1548 Size: 5497 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2295 4108 Size: 107 122 71 631 605 343 280 1813 Cuts at: 4108 136 Size: 1525 Fragments arranged by size: 1813 1525 631 605 343 280 122 107 71 BglII A'GATC_T Cuts at: 5492 5492 Size: 5497 BlpI GC'TnA_GC Cuts at: 2213 2914 2213 Size: 701 4796 Bme1580I G_kGCm'C Cuts at: 1790 3558 4714 1790 Size: 1768 1156 2573 Fragments arranged by size: 2573 1768 1156 BmgBI CAC'GTC Cuts at: 1318 2086 2161 1318 Size: 768 75 4654 Fragments arranged by size: 4654 768 75 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3228 4186 442 Size: 416 53 83 399 24 1811 958 1753 Fragments arranged by size: 1811 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5497 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2378 2540 4157 1239 Size: 794 345 162 1617 2579 Fragments arranged by size: 2579 1617 794 345 162 Bpu10I CC'TnA_GC Cuts at: 2223 2223 Size: 5497 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3622 4487 4785 3622 Size: 865 298 4334 Fragments arranged by size: 4334 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2112 4160 882 Size: 617 613 2048 2219 Fragments arranged by size: 2219 2048 617 613 BsaAI yAC'GTr Cuts at: 493 1204 3288 493 Size: 711 2084 2702 Fragments arranged by size: 2702 2084 711 BsaBI GATnn'nnATC Cuts at: 5491 5491 Size: 5497 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2298 3304 3686 4321 Size: 53 83 186 936 765 1006 382 635 Cuts at: 4321 275 Size: 1451 Fragments arranged by size: 1451 1006 936 765 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2559 2693 3930 4328 4602 4749 5160 2559 Size: 134 1237 398 274 147 411 2896 Fragments arranged by size: 2896 1237 411 398 274 147 134 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 1178 Size: 30 165 30 1176 30 4066 Fragments arranged by size: 4066 1176 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2421 2476 2662 1186 Size: 1235 55 186 4021 Fragments arranged by size: 4021 1235 186 55 BseYI C'CCAG_C Cuts at: 2425 2620 4700 5196 2425 Size: 195 2080 496 2726 Fragments arranged by size: 2726 2080 496 195 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 1163 Size: 573 621 4303 Fragments arranged by size: 4303 621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2473 2947 3708 4736 1540 Size: 275 186 472 474 761 1028 2301 Fragments arranged by size: 2301 1028 761 474 472 275 186 BsiHKAI G_wGCw'C Cuts at: 2214 2229 2607 3558 3643 4714 2214 Size: 15 378 951 85 1071 2997 Fragments arranged by size: 2997 1071 951 378 85 15 BsmI GAATG_Cn' Cuts at: 3008 3101 3008 Size: 93 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2229 2574 2607 2654 2816 3558 Size: 399 424 15 345 33 47 162 742 Cuts at: 3558 3643 4714 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 345 162 85 47 33 15 BspEI T'CCGG_A Cuts at: 2559 2693 5160 2559 Size: 134 2467 2896 Fragments arranged by size: 2896 2467 134 BspHI T'CATG_A Cuts at: 3386 5116 3386 Size: 1730 3767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2319 2335 2544 2785 2852 844 Size: 1475 16 209 241 67 3489 Fragments arranged by size: 3489 1475 241 209 67 16 BsrBI CCG'CTC Cuts at: 1394 1823 3384 1394 Size: 429 1561 3507 Fragments arranged by size: 3507 1561 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3992 4166 65 Size: 1183 2744 174 1396 Fragments arranged by size: 2744 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4141 4328 1700 Size: 110 2331 187 2869 Fragments arranged by size: 2869 2331 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5497 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5497 BssSI C'ACGA_G Cuts at: 3557 4569 3557 Size: 1012 4485 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5497 BstBI TT'CG_AA Cuts at: 4301 4301 Size: 5497 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5497 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5497 BstYI r'GATC_y Cuts at: 1972 2385 2562 2827 3194 3579 3596 5048 5134 Size: 413 177 265 367 385 17 1452 86 Cuts at: 5134 5146 5492 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 413 385 367 346 265 177 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2459 2704 2459 Size: 245 5252 BtgI C'CryG_G Cuts at: 513 1065 2004 2499 513 Size: 552 939 495 3511 Fragments arranged by size: 3511 939 552 495 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4758 Fragments arranged by size: 4758 683 56 BtsI GCAGTG_nn' Cuts at: 3013 3807 3827 3013 Size: 794 20 4683 Fragments arranged by size: 4683 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5497 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5462 DraI TTT'AAA Cuts at: 2801 2801 Size: 5497 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4504 809 Size: 507 3188 1802 Fragments arranged by size: 3188 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 2944 3833 Size: 54 1425 50 275 18 498 616 889 Cuts at: 3833 5462 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 616 498 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2944 1537 Size: 275 1132 4090 Fragments arranged by size: 4090 1132 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3427 1353 Size: 2074 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4084 4458 4604 387 Size: 1064 138 2495 374 146 1280 Fragments arranged by size: 2495 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2227 2227 Size: 5497 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2096 2378 2540 3560 4157 4944 Size: 393 401 63 282 162 1020 597 787 Cuts at: 4944 5212 1239 Size: 268 1524 Fragments arranged by size: 1524 1020 787 597 401 393 282 268 162 63 EcoNI CCTnn'n_nnAGG Cuts at: 2375 2375 Size: 5497 EcoO109I rG'GnC_Cy Cuts at: 2233 2484 2741 2233 Size: 251 257 4989 Fragments arranged by size: 4989 257 251 EcoRI G'AATT_C Cuts at: 2806 3184 2806 Size: 378 5119 FspI TGC'GCA Cuts at: 4003 4003 Size: 5497 HaeII r_GCGC'y Cuts at: 1678 4644 1678 Size: 2966 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2579 2592 2611 2624 4138 Size: 32 135 32 1007 13 19 13 1514 Cuts at: 4138 4170 4212 4244 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1514 1007 135 42 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2158 2841 3087 669 Size: 988 281 220 683 246 3079 Fragments arranged by size: 3079 988 683 281 246 220 HindIII A'AGCT_T Cuts at: 748 748 Size: 5497 HpaI GTT'AAC Cuts at: 3087 3087 Size: 5497 KpnI G_GTAC'C Cuts at: 2822 2822 Size: 5497 MfeI C'AATT_G Cuts at: 3096 3096 Size: 5497 MluI A'CGCG_T Cuts at: 5373 5373 Size: 5497 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2413 4611 4795 1305 Size: 1108 2198 184 2007 Fragments arranged by size: 2198 2007 1108 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2330 10 Size: 54 1425 343 498 3177 Fragments arranged by size: 3177 1425 498 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2498 3455 3814 3973 518 Size: 648 1332 957 359 159 2042 Fragments arranged by size: 2042 1332 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2528 2913 3590 4738 4983 1606 Size: 922 385 677 1148 245 2120 Fragments arranged by size: 2120 1148 922 677 385 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4006 Fragments arranged by size: 4006 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5497 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5497 NotI GC'GGCC_GC Cuts at: 2944 2944 Size: 5497 NspI r_CATG'y Cuts at: 1132 1229 2679 2855 4400 1132 Size: 97 1450 176 1545 2229 Fragments arranged by size: 2229 1545 1450 176 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5497 PciI A'CATG_T Cuts at: 4396 4396 Size: 5497 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5497 PmeI GTTT'AAAC Cuts at: 2801 2801 Size: 5497 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3566 3598 5079 5111 3566 Size: 32 1481 32 3952 Fragments arranged by size: 3952 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2233 2233 Size: 5497 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5497 PsiI TTA'TAA Cuts at: 3067 3067 Size: 5497 PspXI vC'TCGA_Gb Cuts at: 2228 2403 2228 Size: 175 5322 PstI C_TGCA'G Cuts at: 830 2142 2346 2400 2555 2849 830 Size: 1312 204 54 155 294 3478 Fragments arranged by size: 3478 1312 294 204 155 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5497 SacI G_AGCT'C Cuts at: 2229 2229 Size: 5497 SalI G'TCGA_C Cuts at: 1936 2839 1936 Size: 903 4594 SbfI CC_TGCA'GG Cuts at: 2849 2849 Size: 5497 ScaI AGT'ACT Cuts at: 3745 5457 3745 Size: 1712 3785 SfcI C'TryA_G Cuts at: 826 1045 2138 2342 2396 2551 2845 3980 4661 Size: 219 1093 204 54 155 294 1135 681 Cuts at: 4661 4852 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1093 681 294 219 204 191 155 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5497 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5497 SmaI CCC'GGG Cuts at: 1872 2632 2824 1872 Size: 760 192 4545 Fragments arranged by size: 4545 760 192 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 2403 3601 4502 4764 820 Size: 197 926 285 175 1198 901 262 1553 Fragments arranged by size: 1553 1198 926 901 285 262 197 175 SnaBI TAC'GTA Cuts at: 493 3288 493 Size: 2795 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5497 SphI G_CATG'C Cuts at: 1132 2679 2855 1132 Size: 1547 176 3774 Fragments arranged by size: 3774 1547 176 SspI AAT'ATT Cuts at: 5 52 3421 5 Size: 47 3369 2081 Fragments arranged by size: 3369 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5497 StyI C'CwwG_G Cuts at: 513 1065 2004 2293 2756 2936 513 Size: 552 939 289 463 180 3074 Fragments arranged by size: 3074 939 552 463 289 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2653 3524 3541 3694 3879 4324 325 Size: 1255 1073 871 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1073 871 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3743 5455 96 Size: 274 80 33 51 1443 1766 1712 138 Fragments arranged by size: 1766 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5457 5489 5457 Size: 32 5465 Tth111I GACn'n_nGTC Cuts at: 1316 2183 1316 Size: 867 4630 XbaI T'CTAG_A Cuts at: 2833 2833 Size: 5497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2273 2273 Size: 5497 XhoI C'TCGA_G Cuts at: 1943 2228 2403 1943 Size: 285 175 5037 Fragments arranged by size: 5037 285 175 XmaI C'CCGG_G Cuts at: 1870 2630 2822 1870 Size: 760 192 4545 Fragments arranged by size: 4545 760 192 XmnI GAAnn'nnTTC Cuts at: 3626 3626 Size: 5497 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3305 276 Size: 53 83 186 936 1771 2468 Fragments arranged by size: 2468 1771 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI