################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TaqI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 433 CGCG CG^CG AcyI 290 GGCGCC G[GA]^CG[CT]C AfaI 234 GTAC GT^AC ApaI 570 GGGCCC GGGCC^C AspHI 631 GAGCTC G[AT]GC[AT]^C AvaI 644 CTCGGG C^[CT]CG[GA]G BbeI 290 GGCGCC GGCGC^C BsaHI 290 GGCGCC G[GA]^CG[CT]C BsgI 619 CTGCAC GTGCAG(16/14) BsiHKAI 631 GAGCTC G[AT]GC[AT]^C BsmAI 314 GTCTC GTCTC(1/5) BsmBI 313 CGTCTC CGTCTC(1/5) BsmFI 370 GGGAC GGGAC(10/14) BsoBI 644 CTCGGG C^[CT]CG[GA]G BspMI 215 ACCTGC ACCTGC(4/8) BsrI 552 ACTGG ACTGG(1/-1) BstAPI 120 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 401 GGATG GGATG(2/0) BstUI 433 CGCG CG^CG BstXI 416 CCATGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 436 GATC ^GATC EarI 505 GAAGAG CTCTTC(1/4) EcoNI 483 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 654 GCGGG CCCGC(4/6) FokI 401 GGATG GGATG(9/13) HgaI 312 GCGTC GACGC(5/10) Hin1I 290 GGCGCC G[GA]^CG[CT]C HphI 213 TCACC GGTGA(8/7) KasI 290 GGCGCC G^GCGCC Ksp632I 505 GAAGAG CTCTTC(1/4) MboI 436 GATC ^GATC MboII 505 GAAGA GAAGA(8/7) MvnI 433 CGCG CG^CG NarI 290 GGCGCC GG^CGCC NdeII 436 GATC ^GATC PflMI 459 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 370 GGGACCC [GA]G^G[AT]CC[CT] RsaI 234 GTAC GT^AC SacI 631 GAGCTC GAGCT^C Sau3AI 436 GATC ^GATC SbfI 117 CCTGCAGG CCTGCA^GG SfoI 290 GGCGCC GGC^GCC Sse8387I 117 CCTGCAGG CCTGCA^GG StuI 284 AGGCCT AGG^CCT TspRI 551 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 459 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AlwNI 121 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 460 CAGGTGCTG BanI 290 GGCGCC G^G[CT][GA]CC 446 GGCACC BlpI 33 GCTCAGC GC^T[ACGT]AGC 111 GCTGAGC Bpu1102I 33 GCTCAGC GC^T[ACGT]AGC 111 GCTGAGC CelII 33 GCTCAGC GC^T[ACGT]AGC 111 GCTGAGC CfoI 291 GCGC GCG^C 560 GCGC HaeII 290 GGCGCC [GA]GCGC^[CT] 559 AGCGCC HhaI 291 GCGC GCG^C 560 GCGC HinP1I 291 GCGC G^CGC 560 GCGC PvuII 109 CAGCTG CAG^CTG 406 CAGCTG SfaNI 402 GATGC GCATC(5/9) 153 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfcI StyI TfiI TseI