(Circular) (Six-base) MAPSORT of: pFN21AE3081.seq Check: 6639 from: 1 to: 5434 pFN21AE3081 5434 bp With 182 enzymes: SgfI * March 28, 2012 12:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2229 2229 Size: 5434 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3244 278 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2777 1937 Size: 840 4594 Acc65I G'GTAC_C Cuts at: 2755 2755 Size: 5434 AclI AA'CG_TT Cuts at: 1563 3561 3934 1563 Size: 1998 373 3063 Fragments arranged by size: 3063 1998 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 3497 4881 5149 1632 Size: 695 1170 1384 268 1917 Fragments arranged by size: 1917 1384 1170 695 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5237 AflIII A'CryG_T Cuts at: 1203 4333 5310 1203 Size: 3130 977 1327 Fragments arranged by size: 3130 1327 977 AgeI A'CCGG_T Cuts at: 4265 4265 Size: 5434 AhdI GACnn_n'nnGTC Cuts at: 4163 4163 Size: 5434 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5434 AlwNI CAG_nnn'CTG Cuts at: 2131 2470 3177 4749 2131 Size: 339 707 1572 2816 Fragments arranged by size: 2816 1572 707 339 ApaI G_GGCC'C Cuts at: 2579 2579 Size: 5434 ApaLI G'TGCA_C Cuts at: 3491 4647 3491 Size: 1156 4278 ApoI r'AATT_y Cuts at: 1493 2743 2969 3121 5230 1493 Size: 1250 226 152 2109 1697 Fragments arranged by size: 2109 1697 1250 226 152 AseI AT'TA_AT Cuts at: 160 1032 3988 160 Size: 872 2956 1606 Fragments arranged by size: 2956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 AvaI C'yCGr_G Cuts at: 1870 1943 2649 2759 1870 Size: 73 706 110 4545 Fragments arranged by size: 4545 706 110 73 BamHI G'GATC_C Cuts at: 2764 3131 2764 Size: 367 5067 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2295 2451 2755 4210 618 Size: 325 217 627 508 156 304 1455 1842 Fragments arranged by size: 1842 1455 627 508 325 304 217 156 BanII G_rGCy'C Cuts at: 1391 2579 2640 2726 2753 1391 Size: 1188 61 86 27 4072 Fragments arranged by size: 4072 1188 86 61 27 BbeI G_GCGC'C Cuts at: 2299 2299 Size: 5434 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3625 3659 1903 Size: 34 1688 34 3678 Fragments arranged by size: 3678 1688 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3326 4536 3326 Size: 1210 4224 BclI T'GATC_A Cuts at: 1548 1548 Size: 5434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4045 136 Size: 107 122 71 631 605 2373 1525 Fragments arranged by size: 2373 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5429 5429 Size: 5434 BlpI GC'TnA_GC Cuts at: 2039 2117 2851 2039 Size: 78 734 4622 Fragments arranged by size: 4622 734 78 Bme1580I G_kGCm'C Cuts at: 1790 2557 2579 2691 3495 4651 1790 Size: 767 22 112 804 1156 2573 Fragments arranged by size: 2573 1156 804 767 112 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2567 3165 4123 Size: 416 53 83 399 24 1150 598 958 Cuts at: 4123 442 Size: 1753 Fragments arranged by size: 1753 1150 958 598 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5434 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2279 2279 Size: 5434 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4094 1239 Size: 2855 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3559 4424 4722 3559 Size: 865 298 4271 Fragments arranged by size: 4271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4097 882 Size: 617 2598 2219 Fragments arranged by size: 2598 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3225 493 Size: 711 2021 2702 Fragments arranged by size: 2702 2021 711 BsaBI GATnn'nnATC Cuts at: 5428 5428 Size: 5434 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2296 3241 3623 4258 Size: 53 83 186 936 763 945 382 635 Cuts at: 4258 275 Size: 1451 Fragments arranged by size: 1451 945 936 763 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3867 4265 4539 4686 5097 3867 Size: 398 274 147 411 4204 Fragments arranged by size: 4204 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5209 Fragments arranged by size: 5209 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 2513 2516 2519 2528 2531 2534 2537 Size: 1324 3 3 3 9 3 3 3 Cuts at: 2537 2540 2543 2594 1186 Size: 3 3 51 4026 Fragments arranged by size: 4026 1324 51 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2112 2362 2371 2598 2684 2727 4637 5133 2112 Size: 250 9 227 86 43 1910 496 2413 Fragments arranged by size: 2413 1910 496 250 227 86 43 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 1163 Size: 573 873 3988 Fragments arranged by size: 3988 873 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2884 3645 4673 1540 Size: 275 186 883 761 1028 2301 Fragments arranged by size: 2301 1028 883 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2640 3495 3580 4651 2640 Size: 855 85 1071 3423 Fragments arranged by size: 3423 1071 855 85 BsmI GAATG_Cn' Cuts at: 2945 3038 2945 Size: 93 5341 BsmBI CGTCTCn'nnnn_ Cuts at: 2324 2324 Size: 5434 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2557 2579 2640 2691 2726 2753 3495 Size: 399 767 22 61 51 35 27 742 Cuts at: 3495 3580 4651 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 767 742 399 85 61 51 35 27 22 BspEI T'CCGG_A Cuts at: 5097 5097 Size: 5434 BspHI T'CATG_A Cuts at: 3323 5053 3323 Size: 1730 3704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2229 2789 844 Size: 1385 560 3489 Fragments arranged by size: 3489 1385 560 BsrBI CCG'CTC Cuts at: 1394 1823 3321 1394 Size: 429 1498 3507 Fragments arranged by size: 3507 1498 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3929 4103 65 Size: 1183 2681 174 1396 Fragments arranged by size: 2681 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4078 4265 1700 Size: 110 2268 187 2869 Fragments arranged by size: 2869 2268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5434 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5434 BssSI C'ACGA_G Cuts at: 3494 4506 3494 Size: 1012 4422 BstAPI GCAn_nnn'nTGC Cuts at: 794 2131 794 Size: 1337 4097 BstBI TT'CG_AA Cuts at: 4238 4238 Size: 5434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5434 BstXI CCAn_nnnn'nTGG Cuts at: 2428 2428 Size: 5434 BstYI r'GATC_y Cuts at: 1972 2764 3131 3516 3533 4985 5071 5083 5429 Size: 792 367 385 17 1452 86 12 346 Cuts at: 5429 1972 Size: 1977 Fragments arranged by size: 1977 1452 792 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3943 Fragments arranged by size: 3943 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4695 Fragments arranged by size: 4695 683 56 BtsI GCAGTG_nn' Cuts at: 2950 3744 3764 2950 Size: 794 20 4620 Fragments arranged by size: 4620 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5399 DraI TTT'AAA Cuts at: 2738 2738 Size: 5434 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4441 809 Size: 507 3125 1802 Fragments arranged by size: 3125 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2881 3770 5399 Size: 54 1425 50 275 18 1051 889 1629 Cuts at: 5399 8 Size: 43 Fragments arranged by size: 1629 1425 1051 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2881 1537 Size: 275 1069 4090 Fragments arranged by size: 4090 1069 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2505 3364 1353 Size: 1152 859 3423 Fragments arranged by size: 3423 1152 859 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4021 4395 4541 387 Size: 1064 138 2432 374 146 1280 Fragments arranged by size: 2432 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2638 2638 Size: 5434 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 3497 4094 4881 5149 1239 Size: 393 695 1170 597 787 268 1524 Fragments arranged by size: 1524 1170 787 695 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2492 2492 Size: 5434 EcoO109I rG'GnC_Cy Cuts at: 2097 2109 2376 2575 2097 Size: 12 267 199 4956 Fragments arranged by size: 4956 267 199 12 EcoRI G'AATT_C Cuts at: 2743 3121 2743 Size: 378 5056 FspI TGC'GCA Cuts at: 3940 3940 Size: 5434 HaeII r_GCGC'y Cuts at: 1678 2299 2568 4581 1678 Size: 621 269 2013 2531 Fragments arranged by size: 2531 2013 621 269 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2472 2504 4075 4107 4149 Size: 32 135 32 900 32 1571 32 42 Cuts at: 4149 4181 1373 Size: 32 2626 Fragments arranged by size: 2626 1571 900 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2778 3024 669 Size: 988 281 840 246 3079 Fragments arranged by size: 3079 988 840 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5434 HpaI GTT'AAC Cuts at: 3024 3024 Size: 5434 KasI G'GCGC_C Cuts at: 2295 2295 Size: 5434 KpnI G_GTAC'C Cuts at: 2759 2759 Size: 5434 MfeI C'AATT_G Cuts at: 3033 3033 Size: 5434 MluI A'CGCG_T Cuts at: 5310 5310 Size: 5434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4548 4732 1305 Size: 3243 184 2007 Fragments arranged by size: 3243 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3612 Fragments arranged by size: 3612 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3392 3751 3910 518 Size: 648 2226 359 159 2042 Fragments arranged by size: 2226 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 2388 2413 2449 2850 3527 4675 4920 Size: 510 272 25 36 401 677 1148 245 Cuts at: 4920 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 510 401 272 245 36 25 NarI GG'CG_CC Cuts at: 2296 2296 Size: 5434 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3943 Fragments arranged by size: 3943 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5434 NotI GC'GGCC_GC Cuts at: 2881 2881 Size: 5434 NspI r_CATG'y Cuts at: 1132 1229 2792 4337 1132 Size: 97 1563 1545 2229 Fragments arranged by size: 2229 1563 1545 97 PasI CC'CwG_GG Cuts at: 1348 2102 2251 2380 2549 1348 Size: 754 149 129 169 4233 Fragments arranged by size: 4233 754 169 149 129 PciI A'CATG_T Cuts at: 4333 4333 Size: 5434 PflMI CCAn_nnn'nTGG Cuts at: 1108 2470 1108 Size: 1362 4072 PfoI T'CCnGG_A Cuts at: 2490 2490 Size: 5434 PmeI GTTT'AAAC Cuts at: 2738 2738 Size: 5434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3535 5016 5048 3503 Size: 32 1481 32 3889 Fragments arranged by size: 3889 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2376 2376 Size: 5434 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5434 PsiI TTA'TAA Cuts at: 3004 3004 Size: 5434 PspOMI G'GGCC_C Cuts at: 2575 2575 Size: 5434 PstI C_TGCA'G Cuts at: 830 2127 2448 2479 2786 830 Size: 1297 321 31 307 3478 Fragments arranged by size: 3478 1297 321 307 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5434 PvuII CAG'CTG Cuts at: 2116 2413 2116 Size: 297 5137 SacI G_AGCT'C Cuts at: 2640 2640 Size: 5434 SalI G'TCGA_C Cuts at: 1936 2776 1936 Size: 840 4594 SanDI GG'GwC_CC Cuts at: 2376 2376 Size: 5434 SbfI CC_TGCA'GG Cuts at: 2127 2786 2127 Size: 659 4775 ScaI AGT'ACT Cuts at: 3682 5394 3682 Size: 1712 3722 SfcI C'TryA_G Cuts at: 826 1045 2123 2444 2475 2782 3917 4598 4789 Size: 219 1078 321 31 307 1135 681 191 Cuts at: 4789 826 Size: 1471 Fragments arranged by size: 1471 1135 1078 681 321 307 219 191 31 SfoI GGC'GCC Cuts at: 2297 2297 Size: 5434 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5434 SmaI CCC'GGG Cuts at: 1872 2761 1872 Size: 889 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3538 4439 4701 820 Size: 197 926 1595 901 262 1553 Fragments arranged by size: 1595 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3225 493 Size: 2732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5434 SphI G_CATG'C Cuts at: 1132 2792 1132 Size: 1660 3774 SspI AAT'ATT Cuts at: 5 52 3358 5 Size: 47 3306 2081 Fragments arranged by size: 3306 2081 47 StuI AGG'CCT Cuts at: 1517 2291 1517 Size: 774 4660 StyI C'CwwG_G Cuts at: 513 1065 2004 2026 2093 2631 2679 2703 2873 Size: 552 939 22 67 538 48 24 170 Cuts at: 2873 513 Size: 3074 Fragments arranged by size: 3074 939 552 538 170 67 48 24 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3461 3478 3631 3816 4261 325 Size: 1255 1881 17 153 185 445 1498 Fragments arranged by size: 1881 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3680 5392 96 Size: 274 80 33 51 1443 1703 1712 138 Fragments arranged by size: 1712 1703 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5394 5426 5394 Size: 32 5402 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5434 XbaI T'CTAG_A Cuts at: 2770 2770 Size: 5434 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5434 XmaI C'CCGG_G Cuts at: 1870 2759 1870 Size: 889 4545 XmnI GAAnn'nnTTC Cuts at: 3563 3563 Size: 5434 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3242 276 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI