(Linear) (Six-base) MAPSORT of: KIEE3081.seq Check: 2485 from: 1 to: 729 KIEE3081.orf With 182 enzymes: SgfI * March 22, 2012 16:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 224 729 Size: 224 505 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 729 Size: 322 407 AlwNI CAG_nnn'CTG Cuts at: 0 126 465 729 Size: 126 339 264 Fragments arranged by size: 339 264 126 ApaI G_GGCC'C Cuts at: 0 574 729 Size: 574 155 AvaI C'yCGr_G Cuts at: 0 644 729 Size: 644 85 BanI G'GyrC_C Cuts at: 0 290 446 729 Size: 290 156 283 Fragments arranged by size: 290 283 156 BanII G_rGCy'C Cuts at: 0 574 635 721 729 Size: 574 61 86 8 Fragments arranged by size: 574 86 61 8 BbeI G_GCGC'C Cuts at: 0 294 729 Size: 294 435 BlpI GC'TnA_GC Cuts at: 0 34 112 729 Size: 34 78 617 Fragments arranged by size: 617 78 34 Bme1580I G_kGCm'C Cuts at: 0 552 574 686 729 Size: 552 22 112 43 Fragments arranged by size: 552 112 43 22 BmrI ACTGGGnnnn_n' Cuts at: 0 562 729 Size: 562 167 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 274 729 Size: 274 455 BsaHI Gr'CG_yC Cuts at: 0 291 729 Size: 291 438 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 505 508 511 514 523 526 529 532 Size: 505 3 3 3 9 3 3 3 Cuts at: 532 535 538 589 729 Size: 3 3 51 140 Fragments arranged by size: 505 140 51 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 107 357 366 593 679 722 729 Size: 107 250 9 227 86 43 7 Fragments arranged by size: 250 227 107 86 43 9 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 729 Size: 604 125 BsiHKAI G_wGCw'C Cuts at: 0 635 729 Size: 635 94 BsmBI CGTCTCn'nnnn_ Cuts at: 0 319 729 Size: 319 410 Bsp1286I G_dGCh'C Cuts at: 0 552 574 635 686 721 729 Size: 552 22 61 51 35 8 Fragments arranged by size: 552 61 51 35 22 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 224 729 Size: 224 505 BstAPI GCAn_nnn'nTGC Cuts at: 0 126 729 Size: 126 603 BstXI CCAn_nnnn'nTGG Cuts at: 0 423 729 Size: 423 306 EarI CTCTTCn'nnn_ Cuts at: 0 500 729 Size: 500 229 EcoICRI GAG'CTC Cuts at: 0 633 729 Size: 633 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 729 Size: 322 407 EcoNI CCTnn'n_nnAGG Cuts at: 0 487 729 Size: 487 242 EcoO109I rG'GnC_Cy Cuts at: 0 92 104 371 570 729 Size: 92 12 267 199 159 Fragments arranged by size: 267 199 159 92 12 HaeII r_GCGC'y Cuts at: 0 294 563 729 Size: 294 269 166 Fragments arranged by size: 294 269 166 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 467 499 729 Size: 467 32 230 Fragments arranged by size: 467 230 32 KasI G'GCGC_C Cuts at: 0 290 729 Size: 290 439 MspA1I CmG'CkG Cuts at: 0 111 383 408 444 729 Size: 111 272 25 36 285 Fragments arranged by size: 285 272 111 36 25 NarI GG'CG_CC Cuts at: 0 291 729 Size: 291 438 PasI CC'CwG_GG Cuts at: 0 97 246 375 544 729 Size: 97 149 129 169 185 Fragments arranged by size: 185 169 149 129 97 PflMI CCAn_nnn'nTGG Cuts at: 0 465 729 Size: 465 264 PfoI T'CCnGG_A Cuts at: 0 485 729 Size: 485 244 PpuMI rG'GwC_Cy Cuts at: 0 371 729 Size: 371 358 PspOMI G'GGCC_C Cuts at: 0 570 729 Size: 570 159 PstI C_TGCA'G Cuts at: 0 122 443 474 729 Size: 122 321 31 255 Fragments arranged by size: 321 255 122 31 PvuII CAG'CTG Cuts at: 0 111 408 729 Size: 111 297 321 Fragments arranged by size: 321 297 111 SacI G_AGCT'C Cuts at: 0 635 729 Size: 635 94 SanDI GG'GwC_CC Cuts at: 0 371 729 Size: 371 358 SbfI CC_TGCA'GG Cuts at: 0 122 729 Size: 122 607 SfcI C'TryA_G Cuts at: 0 118 439 470 729 Size: 118 321 31 259 Fragments arranged by size: 321 259 118 31 SfoI GGC'GCC Cuts at: 0 292 729 Size: 292 437 StuI AGG'CCT Cuts at: 0 286 729 Size: 286 443 StyI C'CwwG_G Cuts at: 0 21 88 626 674 698 729 Size: 21 67 538 48 24 31 Fragments arranged by size: 538 67 48 31 24 21 Enzymes that do cut: AarI AcuI AlwNI ApaI AvaI BanI BanII BbeI BlpI Bme1580I BmrI BplI BsaHI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BstAPI BstXI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MspA1I NarI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SbfI SfcI SfoI StuI StyI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI