################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII BalI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI Fnu4HI FseI FspI HaeII HgaI Hin1I HpaI ItaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I TseI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccII 995 CGCG CG^CG Asp700I 720 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1078 GTGCTC G[AT]GC[AT]^C AspI 906 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 27 CCTAGG C^CTAGG BamHI 452 GGATCC G^GATCC BanI 970 GGCACC G^G[CT][GA]CC BlnI 27 CCTAGG C^CTAGG BseRI 145 CTCCTC GAGGAG(10/8) BsiHKAI 1078 GTGCTC G[AT]GC[AT]^C Bsp1407I 551 TGTACA T^GTACA BspHI 797 TCATGA T^CATGA BsrDI 431 GCAATG GCAATG(2/0) BsrGI 551 TGTACA T^GTACA BssSI 490 CACGAG CACGAG(-5/-1) BstUI 995 CGCG CG^CG BstXI 512 CCAACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 452 GGATCC [GA]^GATC[CT] DrdI 787 GACTTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 474 GAATTC G^AATTC FauI 993 CCCGC CCCGC(4/6) HaeIII 115 GGCC GG^CC HindIII 691 AAGCTT A^AGCTT MaeII 964 ACGT A^CGT MflI 452 GGATCC [GA]^GATC[CT] MvnI 995 CGCG CG^CG MwoI 188 GCAGAGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 593 ACATGC [GA]CATG^[CT] PflFI 906 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 126 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 797 TCATGA T^CATGA SapI 723 GCTCTTC GCTCTTC(1/4) SexAI 373 ACCTGGT A^CC[AT]GGT SspBI 551 TGTACA T^GTACA Tth111I 906 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 126 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 956 CCATGACCACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 452 GGATCC [GA]^GATC[CT] XmnI 720 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 378 GTAC GT^AC 552 GTAC AlwNI 127 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 908 CAGAGTCTG BbsI 22 GTCTTC GAAGAC(2/6) 1045 GTCTTC BcnI 456 CCCGG CC^[GC]GG 923 CCCGG BmyI 969 GGGCAC G[AGT]GC[ACT]^C 1078 GTGCTC BpmI 46 CTCCAG CTGGAG(16/14) 917 CTCCAG BpuAI 22 GTCTTC GAAGAC(2/6) 1045 GTCTTC BsmFI 63 GGGAC GGGAC(10/14) 856 GTCCC Bsp1286I 969 GGGCAC G[AGT]GC[ACT]^C 1078 GTGCTC Cac8I 443 GCCAGC GC[ACGT]^[ACGT]GC 821 GCAGGC CfoI 258 GCGC GCG^C 1058 GCGC DraII 63 GGGACCT [GA]G^G[ACGT]CC[CT] 484 AGGACCC EarI 93 CTCTTC CTCTTC(1/4) 724 CTCTTC EcoNI 147 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 464 CCTTTTTTAGG EcoO109I 63 GGGACCT [GA]G^G[ACGT]CC[CT] 484 AGGACCC HhaI 258 GCGC GCG^C 1058 GCGC HinP1I 258 GCGC G^CGC 1058 GCGC HincII 165 GTCAAC GT[CT]^[GA]AC 307 GTCAAC HindII 165 GTCAAC GT[CT]^[GA]AC 307 GTCAAC Ksp632I 93 CTCTTC CTCTTC(1/4) 724 CTCTTC MslI 490 CACGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 792 CATGGTCATG NciI 456 CCCGG CC^[GC]GG 923 CCCGG PleI 412 GAGTC GAGTC(4/5) 910 GAGTC PpuMI 63 GGGACCT [GA]G^G[AT]CC[CT] 484 AGGACCC RsaI 378 GTAC GT^AC 552 GTAC TfiI 76 GAATC G^A[AT]TC 613 GATTC TspRI 282 CACTG CA[GC]TG[ACGT][ACGT]^ 291 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BfaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII EcoT14I FokI HapII HinfI HpaII HphI MaeI MaeIII MboI MboII MnlI MspI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tsp45I Tsp509I TthHB8I