(Circular) (Six-base) MAPSORT of: pFN21AE5538.seq Check: 8532 from: 1 to: 5800 pFN21AE5538 5800 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3863 5247 5515 1632 Size: 2231 1384 268 1917 Fragments arranged by size: 2231 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AlwNI CAG_nnn'CTG Cuts at: 2137 2918 3543 5115 2137 Size: 781 625 1572 2822 Fragments arranged by size: 2822 1572 781 625 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 2479 2689 2992 3109 3335 3487 5596 1493 Size: 986 210 303 117 226 152 2109 1697 Fragments arranged by size: 2109 1697 986 303 226 210 152 117 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3125 1870 Size: 73 1182 4545 Fragments arranged by size: 4545 1182 73 AvrII C'CTAG_G Cuts at: 2032 2032 Size: 5800 BamHI G'GATC_C Cuts at: 2457 3130 3497 2457 Size: 673 367 4760 Fragments arranged by size: 4760 673 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2975 3121 4576 618 Size: 325 217 627 1188 146 1455 1842 Fragments arranged by size: 1842 1455 1188 627 325 217 146 BanII G_rGCy'C Cuts at: 1391 3119 1391 Size: 1728 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2020 3043 928 Size: 971 121 1023 3685 Fragments arranged by size: 3685 1023 971 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4411 136 Size: 107 122 71 631 605 2739 1525 Fragments arranged by size: 2739 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2978 3861 5017 1790 Size: 1188 883 1156 2573 Fragments arranged by size: 2573 1188 1156 883 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2196 2777 3531 Size: 416 53 83 399 24 779 581 754 Cuts at: 3531 4489 442 Size: 958 1753 Fragments arranged by size: 1753 958 779 754 581 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2734 2734 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2907 4460 1239 Size: 797 871 1553 2579 Fragments arranged by size: 2579 1553 871 797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3607 3989 4624 275 Size: 53 83 186 936 2074 382 635 1451 Fragments arranged by size: 2074 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5575 Fragments arranged by size: 5575 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 1186 Size: 955 4845 BseYI C'CCAG_C Cuts at: 2124 2130 5003 5499 2124 Size: 6 2873 496 2425 Fragments arranged by size: 2873 2425 496 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5227 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3087 3861 3946 5017 3087 Size: 774 85 1071 3870 Fragments arranged by size: 3870 1071 774 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2978 3087 3119 3861 3946 5017 1391 Size: 399 1188 109 32 742 85 1071 2174 Fragments arranged by size: 2174 1188 1071 742 399 109 85 32 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 2802 3689 5419 2802 Size: 887 1730 3183 Fragments arranged by size: 3183 1730 887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3155 844 Size: 2311 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2443 4295 4469 65 Size: 1183 1195 1852 174 1396 Fragments arranged by size: 1852 1396 1195 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 2556 96 Size: 2460 3340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 2495 3860 4872 2495 Size: 1365 1012 3423 Fragments arranged by size: 3423 1365 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2524 2524 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2457 3130 3497 3882 3899 5351 5437 5449 Size: 485 673 367 385 17 1452 86 12 Cuts at: 5449 5795 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 673 485 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5248 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 3316 4110 4130 3316 Size: 794 20 4986 Fragments arranged by size: 4986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2798 4807 809 Size: 507 1482 2009 1802 Fragments arranged by size: 2009 1802 1482 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3247 4136 5765 Size: 54 1425 50 275 18 1417 889 1629 Cuts at: 5765 8 Size: 43 Fragments arranged by size: 1629 1425 1417 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2104 2735 3730 1353 Size: 751 631 995 3423 Fragments arranged by size: 3423 995 751 631 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2907 3863 4460 5247 5515 1239 Size: 393 404 871 956 597 787 268 1524 Fragments arranged by size: 1524 956 871 787 597 404 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2156 2473 2156 Size: 317 5483 EcoO109I rG'GnC_Cy Cuts at: 2069 2490 2069 Size: 421 5379 EcoRI G'AATT_C Cuts at: 2479 3109 3487 2479 Size: 630 378 4792 Fragments arranged by size: 4792 630 378 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 4947 1678 Size: 3269 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2577 2609 2641 4441 4473 Size: 32 135 32 1005 32 32 1800 32 Cuts at: 4473 4515 4547 1373 Size: 42 32 2626 Fragments arranged by size: 2626 1800 1005 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2172 2314 3144 3390 669 Size: 988 281 234 142 830 246 3079 Fragments arranged by size: 3079 988 830 281 246 234 142 HindIII A'AGCT_T Cuts at: 748 2696 748 Size: 1948 3852 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3051 4914 5098 1305 Size: 1746 1863 184 2007 Fragments arranged by size: 2007 1863 1746 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2499 2801 3758 4117 4276 518 Size: 648 1333 302 957 359 159 2042 Fragments arranged by size: 2042 1333 957 648 359 302 159 MspA1I CmG'CkG Cuts at: 1606 3216 3893 5041 5286 1606 Size: 1610 677 1148 245 2120 Fragments arranged by size: 2120 1610 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5248 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 2602 3158 4703 1132 Size: 97 1373 556 1545 2229 Fragments arranged by size: 2229 1545 1373 556 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 2137 1108 Size: 1029 4771 PfoI T'CCnGG_A Cuts at: 2460 2720 2460 Size: 260 5540 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2069 2490 2069 Size: 421 5379 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PstI C_TGCA'G Cuts at: 830 3152 830 Size: 2322 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SapI GCTCTTCn'nnn_ Cuts at: 2735 2735 Size: 5800 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SexAI A'CCwGG_T Cuts at: 2378 2378 Size: 5800 SfcI C'TryA_G Cuts at: 826 1045 3148 4283 4964 5155 826 Size: 219 2103 1135 681 191 1471 Fragments arranged by size: 2103 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3904 4805 5067 820 Size: 197 926 1961 901 262 1553 Fragments arranged by size: 1961 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5800 StyI C'CwwG_G Cuts at: 513 1065 2032 2320 3021 3239 513 Size: 552 967 288 701 218 3074 Fragments arranged by size: 3074 967 701 552 288 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3827 3844 3997 4182 4627 325 Size: 1255 2247 17 153 185 445 1498 Fragments arranged by size: 2247 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 4046 5758 Size: 274 80 33 51 1443 579 1490 1712 Cuts at: 5758 96 Size: 138 Fragments arranged by size: 1712 1490 1443 579 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 2914 1316 Size: 1598 4202 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2968 2968 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 2729 3929 2729 Size: 1200 4600 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI