################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI BstXI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 633 GTATAC GT^[AC][GT]AC AcsI 374 GAATTC [GA]^AATT[CT] AlwI 580 GATCC GGATC(4/5) ApoI 374 GAATTC [GA]^AATT[CT] AvaII 234 GGACC G^G[AT]CC BanI 567 GGTGCC G^G[CT][GA]CC BbsI 174 GAAGAC GAAGAC(2/6) BcnI 540 CCCGG CC^[GC]GG BfaI 94 CTAG C^TAG BmyI 568 GTGCCC G[AGT]GC[ACT]^C BpmI 154 CTGGAG CTGGAG(16/14) BpuAI 174 GAAGAC GAAGAC(2/6) BsmAI 157 GAGAC GTCTC(1/5) Bsp1286I 568 GTGCCC G[AGT]GC[ACT]^C BssSI 29 CTCGTG CACGAG(-5/-1) Bst1107I 633 GTATAC GTA^TAC BstNI 238 CCTGG CC^[AT]GG BstZ17I 633 GTATAC GTA^TAC DdeI 430 CTCAG C^T[ACGT]AG DsaI 612 CCATGG C^C[GA][CT]GG EcoRI 374 GAATTC G^AATTC EcoRII 238 CCTGG ^CC[AT]GG EcoRV 256 GATATC GAT^ATC EcoT14I 612 CCATGG C^C[AT][AT]GG HapII 541 CCGG C^CGG HindIII 460 AAGCTT A^AGCTT HpaII 541 CCGG C^CGG MaeI 94 CTAG C^TAG MseI 476 TTAA T^TAA MspA1I 443 CAGCTG C[AC]G^C[GT]G MspI 541 CCGG C^CGG MvaI 238 CCTGG CC^[AT]GG NciI 540 CCCGG CC^[GC]GG NcoI 612 CCATGG C^CATGG NdeI 179 CATATG CA^TATG PpuMI 233 GGGACCC [GA]G^G[AT]CC[CT] PvuII 443 CAGCTG CAG^CTG SfcI 316 CTACAG C^T[GA][CT]AG StyI 612 CCATGG C^C[AT][AT]GG TaqI 438 TCGA T^CGA Tru9I 476 TTAA T^TAA Tsp45I 197 GTGAC ^GT[GC]AC TthHB8I 438 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 6 CGCG CG^CG 248 CGCG BbvI 442 GCAGC GCAGC(8/12) 676 GCTGC BsmFI 233 GGGAC GGGAC(10/14) 293 GTCCC BssKI 238 CCTGG ^CC[ACGT]GG 540 CCCGG BstUI 6 CGCG CG^CG 248 CGCG CfoI 7 GCGC GCG^C 247 GCGC DraII 233 GGGACCC [GA]G^G[ACGT]CC[CT] 601 GGGGCCT EaeI 2 TGGCCG [CT]^GGCC[GA] 250 CGGCCA EcoO109I 233 GGGACCC [GA]G^G[ACGT]CC[CT] 601 GGGGCCT HhaI 7 GCGC GCG^C 247 GCGC HinP1I 7 GCGC G^CGC 247 GCGC HphI 260 TCACC GGTGA(8/7) 683 TCACC MaeIII 197 GTGAC ^GT[ACGT]AC 659 GTAAC MvnI 6 CGCG CG^CG 248 CGCG MwoI 75 GCCACCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 395 GCTTTTGTGGC NlaIII 408 CATG CATG^ 613 CATG ScrFI 238 CCTGG CC^[ACGT]GG 540 CCCGG SfaNI 67 GATGC GCATC(5/9) 279 GATGC TseI 442 GCAGC G^C[AT]GC 676 GCTGC Tsp509I 375 AATT ^AATT 474 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BsaJI BsiYI BslI BsrI BstF5I Cac8I Cfr13I DpnII Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MnlI NdeII NlaIV Sau3AI Sau96I TfiI TspRI