(Circular) (Six-base) MAPSORT of: pFN21AE4327.seq Check: 7011 from: 1 to: 5701 pFN21AE4327 5701 bp With 182 enzymes: SgfI * March 28, 2012 14:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2288 2288 Size: 5701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3044 1937 Size: 1107 4594 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3764 5148 5416 1632 Size: 2132 1384 268 1917 Fragments arranged by size: 2132 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 4600 5577 1203 Size: 3397 977 1327 Fragments arranged by size: 3397 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 2388 2419 1166 Size: 1222 31 4448 Fragments arranged by size: 4448 1222 31 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2437 1184 Size: 1253 4448 AlwNI CAG_nnn'CTG Cuts at: 2044 2352 3444 5016 2044 Size: 308 1092 1572 2729 Fragments arranged by size: 2729 1572 1092 308 ApaI G_GGCC'C Cuts at: 2124 2982 2124 Size: 858 4843 ApaLI G'TGCA_C Cuts at: 2412 3758 4914 2412 Size: 1346 1156 3199 Fragments arranged by size: 3199 1346 1156 ApoI r'AATT_y Cuts at: 1493 3010 3236 3388 5497 1493 Size: 1517 226 152 2109 1697 Fragments arranged by size: 2109 1697 1517 226 152 AseI AT'TA_AT Cuts at: 160 1032 4255 160 Size: 872 3223 1606 Fragments arranged by size: 3223 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 3026 1870 Size: 73 1083 4545 Fragments arranged by size: 4545 1083 73 BamHI G'GATC_C Cuts at: 3031 3398 3031 Size: 367 5334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 3022 4477 618 Size: 325 217 627 396 839 1455 1842 Fragments arranged by size: 1842 1455 839 627 396 325 217 BanII G_rGCy'C Cuts at: 1391 2124 2345 2982 3020 1391 Size: 733 221 637 38 4072 Fragments arranged by size: 4072 733 637 221 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4730 BbvCI CC'TCA_GC Cuts at: 2125 2125 Size: 5701 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3892 3926 1903 Size: 34 50 34 1871 34 3678 Fragments arranged by size: 3678 1871 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 1548 Size: 5701 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2986 4312 136 Size: 107 122 71 631 605 1314 1326 1525 Fragments arranged by size: 1525 1326 1314 631 605 122 107 71 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 3118 3118 Size: 5701 Bme1580I G_kGCm'C Cuts at: 1790 2124 2186 2416 2982 3762 4918 1790 Size: 334 62 230 566 780 1156 2573 Fragments arranged by size: 2573 1156 780 566 334 230 62 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2125 3432 4390 Size: 416 53 83 399 24 708 1307 958 Cuts at: 4390 442 Size: 1753 Fragments arranged by size: 1753 1307 958 708 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2329 2596 2792 4361 1239 Size: 1090 267 196 1569 2579 Fragments arranged by size: 2579 1569 1090 267 196 Bpu10I CC'TnA_GC Cuts at: 2125 2125 Size: 5701 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4364 882 Size: 617 2865 2219 Fragments arranged by size: 2865 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3492 493 Size: 711 2288 2702 Fragments arranged by size: 2702 2288 711 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3508 3890 4525 275 Size: 53 83 186 936 1975 382 635 1451 Fragments arranged by size: 1975 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4134 4532 4806 4953 5364 4134 Size: 398 274 147 411 4471 Fragments arranged by size: 4471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2223 2253 2569 2599 1178 Size: 30 165 30 820 30 316 30 4280 Fragments arranged by size: 4280 820 316 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2804 2807 1186 Size: 1162 456 3 4080 Fragments arranged by size: 4080 1162 456 3 BseYI C'CCAG_C Cuts at: 2332 2664 4904 5400 2332 Size: 332 2240 496 2633 Fragments arranged by size: 2633 2240 496 332 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2082 2197 1163 Size: 573 346 115 4667 Fragments arranged by size: 4667 573 346 115 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3151 3912 4940 1540 Size: 275 186 1150 761 1028 2301 Fragments arranged by size: 2301 1150 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2416 2444 3762 3847 4918 2416 Size: 28 1318 85 1071 3199 Fragments arranged by size: 3199 1318 1071 85 28 BsmI GAATG_Cn' Cuts at: 3212 3305 3212 Size: 93 5608 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2186 2345 2416 2444 2982 3020 Size: 399 334 62 159 71 28 538 38 Cuts at: 3020 3762 3847 4918 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 399 334 159 85 71 62 38 28 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 3590 5320 3590 Size: 1730 3971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2288 2490 2534 3056 844 Size: 1444 202 44 522 3489 Fragments arranged by size: 3489 1444 522 202 44 BsrBI CCG'CTC Cuts at: 1394 1823 3588 1394 Size: 429 1765 3507 Fragments arranged by size: 3507 1765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4196 4370 65 Size: 1183 2948 174 1396 Fragments arranged by size: 2948 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2989 4345 4532 1700 Size: 110 1179 1356 187 2869 Fragments arranged by size: 2869 1356 1179 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5701 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5701 BssSI C'ACGA_G Cuts at: 2443 2539 3761 4773 2443 Size: 96 1222 1012 3371 Fragments arranged by size: 3371 1222 1012 96 BstAPI GCAn_nnn'nTGC Cuts at: 794 2411 794 Size: 1617 4084 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 2689 1184 Size: 1505 4196 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2374 2011 Size: 363 5338 BstYI r'GATC_y Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696 Size: 1059 367 385 17 1452 86 12 346 Cuts at: 5696 1972 Size: 1977 Fragments arranged by size: 1977 1452 1059 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2168 2432 2168 Size: 264 5437 BtgI C'CryG_G Cuts at: 513 1065 2062 2982 513 Size: 552 997 920 3232 Fragments arranged by size: 3232 997 920 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4962 Fragments arranged by size: 4962 683 56 BtsI GCAGTG_nn' Cuts at: 2042 2412 2444 2478 2560 3217 4011 4031 2042 Size: 370 32 34 82 657 794 20 3712 Fragments arranged by size: 3712 794 657 370 82 34 32 20 ClaI AT'CG_AT Cuts at: 1554 2307 1554 Size: 753 4948 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5666 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4708 809 Size: 507 3392 1802 Fragments arranged by size: 3392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2557 2763 2885 Size: 54 1425 50 275 18 727 206 122 Cuts at: 2885 3148 4037 5666 8 Size: 263 889 1629 43 Fragments arranged by size: 1629 1425 889 727 275 263 206 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3148 1537 Size: 275 1336 4090 Fragments arranged by size: 4090 1336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2366 2640 3631 1353 Size: 1013 274 991 3423 Fragments arranged by size: 3423 1013 991 274 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4288 4662 4808 387 Size: 1064 138 2699 374 146 1280 Fragments arranged by size: 2699 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2329 2596 2792 3764 4361 5148 5416 Size: 393 697 267 196 972 597 787 268 Cuts at: 5416 1239 Size: 1524 Fragments arranged by size: 1524 972 787 697 597 393 268 267 196 EcoO109I rG'GnC_Cy Cuts at: 2120 2121 2164 2861 2978 2120 Size: 1 43 697 117 4843 Fragments arranged by size: 4843 697 117 43 1 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2412 2412 Size: 5701 FspI TGC'GCA Cuts at: 4207 4207 Size: 5701 HaeII r_GCGC'y Cuts at: 1678 4848 1678 Size: 3170 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2223 2255 2569 2601 4342 Size: 32 135 32 651 32 314 32 1741 Cuts at: 4342 4374 4416 4448 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1741 651 314 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3045 3291 669 Size: 988 281 1107 246 3079 Fragments arranged by size: 3079 1107 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5701 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4815 4999 1305 Size: 3510 184 2007 Fragments arranged by size: 3510 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2559 2765 2887 10 Size: 54 1425 343 727 206 122 2824 Fragments arranged by size: 2824 1425 727 343 206 122 54 MslI CAynn'nnrTG Cuts at: 518 1166 2388 2419 3659 4018 4177 518 Size: 648 1222 31 1240 359 159 2042 Fragments arranged by size: 2042 1240 1222 648 359 159 31 MspA1I CmG'CkG Cuts at: 1606 3117 3794 4942 5187 1606 Size: 1511 677 1148 245 2120 Fragments arranged by size: 2120 1511 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5149 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 3148 3148 Size: 5701 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5701 NspI r_CATG'y Cuts at: 1132 1229 2487 3059 4604 1132 Size: 97 1258 572 1545 2229 Fragments arranged by size: 2229 1545 1258 572 97 PasI CC'CwG_GG Cuts at: 1348 2877 1348 Size: 1529 4172 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 2352 1108 Size: 1244 4457 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 3271 3271 Size: 5701 PspOMI G'GGCC_C Cuts at: 2120 2978 2120 Size: 858 4843 PstI C_TGCA'G Cuts at: 830 2052 3053 830 Size: 1222 1001 3478 Fragments arranged by size: 3478 1222 1001 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 SalI G'TCGA_C Cuts at: 1936 3043 1936 Size: 1107 4594 SbfI CC_TGCA'GG Cuts at: 3053 3053 Size: 5701 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SfcI C'TryA_G Cuts at: 826 1045 2048 3049 4184 4865 5056 826 Size: 219 1003 1001 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 1001 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2986 2986 Size: 5701 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3805 4706 4968 820 Size: 197 926 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 3059 1132 Size: 1927 3774 SspI AAT'ATT Cuts at: 5 52 3625 5 Size: 47 3573 2081 Fragments arranged by size: 3573 2081 47 StuI AGG'CCT Cuts at: 1517 2651 1517 Size: 1134 4567 StyI C'CwwG_G Cuts at: 513 1065 2151 2261 2592 3140 513 Size: 552 1086 110 331 548 3074 Fragments arranged by size: 3074 1086 552 548 331 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3728 3745 3898 4083 4528 325 Size: 1255 2148 17 153 185 445 1498 Fragments arranged by size: 2148 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3947 5659 96 Size: 274 80 33 51 1443 1970 1712 138 Fragments arranged by size: 1970 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5661 5693 5661 Size: 32 5669 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5701 XbaI T'CTAG_A Cuts at: 2267 3037 2267 Size: 770 4931 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2485 2880 2485 Size: 395 5306 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5701 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 3830 3830 Size: 5701 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI