################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MscI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 1009 GTAGAC GT^[AC][GT]AC AccIII 305 TCCGGA T^CCGGA AcsI 430 AAATTT [GA]^AATT[CT] AflIII 119 ACATGT A^C[GA][CT]GT AhdI 34 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 327 GTGCAC G^TGCAC ApaLI 327 GTGCAC G^TGCAC ApoI 430 AAATTT [GA]^AATT[CT] AspEI 34 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 616 GAGCTC G[GA]GC[CT]^C BbsI 414 GTCTTC GAAGAC(2/6) BbvCI 820 GCTGAGG CCTCAGC(-5/-2) BclI 225 TGATCA T^GATCA BfaI 790 CTAG C^TAG BpuAI 414 GTCTTC GAAGAC(2/6) BsaWI 305 TCCGGA [AT]^CCGG[AT] BseAI 305 TCCGGA T^CCGGA BspEI 305 TCCGGA T^CCGGA BspHI 522 TCATGA T^CATGA BspLU11I 119 ACATGT A^CATGT BsrBI 64 GAGCGG CCGCTC(-3/-3) BstAPI 314 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 160 CCTGG CC^[AT]GG Cac8I 337 GCCTGC GC[ACGT]^[ACGT]GC CfoI 93 GCGC GCG^C EaeI 67 CGGCCA [CT]^GGCC[GA] Eam1105I 34 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 535 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 160 CCTGG ^CC[AT]GG EcoT14I 896 CCTTGG C^C[AT][AT]GG FbaI 225 TGATCA T^GATCA HhaI 93 GCGC GCG^C HinP1I 93 GCGC G^CGC HincII 932 GTCAAC GT[CT]^[GA]AC HindII 932 GTCAAC GT[CT]^[GA]AC HphI 727 GGTGA GGTGA(8/7) MaeI 790 CTAG C^TAG MroI 305 TCCGGA T^CCGGA MseI 905 TTAA T^TAA MspA1I 81 CAGCTG C[AC]G^C[GT]G MvaI 160 CCTGG CC^[AT]GG NspI 119 ACATGT [GA]CATG^[CT] PvuII 81 CAGCTG CAG^CTG RcaI 522 TCATGA T^CATGA SacI 616 GAGCTC GAGCT^C SmaI 671 CCCGGG CCC^GGG StyI 896 CCTTGG C^C[AT][AT]GG TfiI 700 GAATC G^A[AT]TC Tru9I 905 TTAA T^TAA TspRI 126 CAGTG CA[GC]TG[ACGT][ACGT]^ XmaCI 671 CCCGGG C^CCGGG XmaI 671 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 44 CGCG CG^CG 94 CGCG AciI 45 GCGG CCGC(-3/-1) 66 GCGG AlwI 106 GATCC GGATC(4/5) 87 GGATC AspHI 327 GTGCAC G[AT]GC[AT]^C 616 GAGCTC AvaI 49 CCCGAG C^[CT]CG[GA]G 671 CCCGGG BcnI 671 CCCGG CC^[GC]GG 672 CCGGG BmyI 327 GTGCAC G[AGT]GC[ACT]^C 616 GAGCTC BpmI 555 CTGGAG CTGGAG(16/14) 1001 CTGGAG BseRI 58 GAGGAG GAGGAG(10/8) 61 GAGGAG BsiHKAI 327 GTGCAC G[AT]GC[AT]^C 616 GAGCTC BsmBI 21 GAGACG CGTCTC(1/5) 991 GAGACG BsoBI 49 CCCGAG C^[CT]CG[GA]G 671 CCCGGG Bsp1286I 327 GTGCAC G[AGT]GC[ACT]^C 616 GAGCTC BsrI 554 ACTGG ACTGG(1/-1) 471 CCAGT BstUI 44 CGCG CG^CG 94 CGCG DraII 279 GGGACCC [GA]G^G[ACGT]CC[CT] 882 AGGTCCT EcoO109I 279 GGGACCC [GA]G^G[ACGT]CC[CT] 882 AGGTCCT HaeIII 47 GGCC GG^CC 68 GGCC HapII 306 CCGG C^CGG 672 CCGG HpaII 306 CCGG C^CGG 672 CCGG MspI 306 CCGG C^CGG 672 CCGG MvnI 44 CGCG CG^CG 94 CGCG NciI 671 CCCGG CC^[GC]GG 672 CCGGG PleI 40 GAGTC GAGTC(4/5) 412 GAGTC PpuMI 279 GGGACCC [GA]G^G[AT]CC[CT] 882 AGGTCCT SapI 613 GAAGAGC GCTCTTC(1/4) 687 GAAGAGC SfcI 236 CTACAG C^T[GA][CT]AG 920 CTGTAG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI AvaII BbvI BsaJI BsiYI BslI BsmAI BsmFI BssKI BstF5I Cfr13I DdeI DpnII EarI Fnu4HI FokI HinfI ItaI Ksp632I MaeIII MboI MboII MnlI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp509I