################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI AlwI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV EcoT22I FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI ItaI KasI KpnI Ksp632I KspI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NciI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TseI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 130 GTCGAC GT^[AC][GT]AC AccII 173 CGCG CG^CG AcsI 373 GAATTC [GA]^AATT[CT] Alw44I 527 GTGCAC G^TGCAC AlwNI 580 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 610 CCTGAGG CC^T[ACGT]AGG ApaLI 527 GTGCAC G^TGCAC ApoI 373 GAATTC [GA]^AATT[CT] Asp700I 507 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 527 GTGCAC G[AT]GC[AT]^C BciVI 215 GTATCC GTATCC(6/5) BfaI 649 CTAG C^TAG BmyI 527 GTGCAC G[AGT]GC[ACT]^C BpmI 201 CTCCAG CTGGAG(16/14) BseRI 644 CTCCTC GAGGAG(10/8) BsiHKAI 527 GTGCAC G[AT]GC[AT]^C BsmI 73 GCATTC GAATGC(1/-1) Bsp1286I 527 GTGCAC G[AGT]GC[ACT]^C BspLU11I 117 ACATGT A^CATGT BspMI 314 ACCTGC ACCTGC(4/8) BssKI 69 CCAGG ^CC[ACGT]GG BstNI 69 CCAGG CC^[AT]GG BstUI 173 CGCG CG^CG Bsu36I 610 CCTGAGG CC^T[ACGT]AGG Cac8I 163 GCGGGC GC[ACGT]^[ACGT]GC DsaI 541 CCGTGG C^C[GA][CT]GG Eco81I 610 CCTGAGG CC^T[ACGT]AGG EcoRI 373 GAATTC G^AATTC EcoRII 69 CCAGG ^CC[AT]GG EcoT14I 532 CCTTGG C^C[AT][AT]GG FauI 163 GCGGG CCCGC(4/6) HapII 729 CCGG C^CGG HincII 130 GTCGAC GT[CT]^[GA]AC HindII 130 GTCGAC GT[CT]^[GA]AC HpaII 729 CCGG C^CGG HphI 260 TCACC GGTGA(8/7) MaeI 649 CTAG C^TAG MspA1I 161 CAGCGG C[AC]G^C[GT]G MspI 729 CCGG C^CGG MvaI 69 CCAGG CC^[AT]GG MvnI 173 CGCG CG^CG NspI 117 ACATGT [GA]CATG^[CT] PleI 725 GAGTC GAGTC(4/5) SalI 130 GTCGAC G^TCGAC ScrFI 69 CCAGG CC^[ACGT]GG SmlI 20 CTCAAG C^T[CT][GA]AG StyI 532 CCTTGG C^C[AT][AT]GG TspRI 503 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 507 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 163 GCGG CCGC(-3/-1) 174 GCGG AflIII 117 ACATGT A^C[GA][CT]GT 814 ACGTGT AvaII 536 GGACC G^G[AT]CC 640 GGACC BsmAI 659 GAGAC GTCTC(1/5) 582 GTCTC Bsp1407I 120 TGTACA T^GTACA 817 TGTACA BsrGI 120 TGTACA T^GTACA 817 TGTACA BsrI 302 ACTGG ACTGG(1/-1) 662 ACTGG BstXI 35 CCATAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 741 CCATGTGTTTGG EcoNI 16 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 643 CCTCCTCTAGG HaeIII 166 GGCC GG^CC 731 GGCC MaeII 796 ACGT A^CGT 814 ACGT MslI 36 CATAACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 624 CATACTCATG MwoI 267 GCTGGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 723 GCGAGTCCGGC SspBI 120 TGTACA T^GTACA 817 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AfaI AluI BsaJI BsiYI BslI BsmFI BspHI BstF5I Cfr13I DdeI FokI HinfI MaeIII MnlI MseI NdeI NlaIII NlaIV RcaI RsaI Sau96I SfaNI TaqI TfiI Tru9I Tsp509I TthHB8I XcmI