(Circular) (Six-base) MAPSORT of: pFN21AE5313.seq Check: 6459 from: 1 to: 5587 pFN21AE5313 5587 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3397 278 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2136 2930 1937 Size: 199 794 4594 Fragments arranged by size: 4594 794 199 Acc65I G'GTAC_C Cuts at: 2908 2908 Size: 5587 AclI AA'CG_TT Cuts at: 1563 3714 4087 1563 Size: 2151 373 3063 Fragments arranged by size: 3063 2151 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3650 5034 5302 1632 Size: 2018 1384 268 1917 Fragments arranged by size: 2018 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5390 AflIII A'CryG_T Cuts at: 1203 2122 2819 4486 5463 1203 Size: 919 697 1667 977 1327 Fragments arranged by size: 1667 1327 977 919 697 AgeI A'CCGG_T Cuts at: 4418 4418 Size: 5587 AhdI GACnn_n'nnGTC Cuts at: 4316 4316 Size: 5587 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5587 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5587 AlwNI CAG_nnn'CTG Cuts at: 2590 3330 4902 2590 Size: 740 1572 3275 Fragments arranged by size: 3275 1572 740 ApaLI G'TGCA_C Cuts at: 2532 3644 4800 2532 Size: 1112 1156 3319 Fragments arranged by size: 3319 1156 1112 ApoI r'AATT_y Cuts at: 1493 2378 2896 3122 3274 5383 1493 Size: 885 518 226 152 2109 1697 Fragments arranged by size: 2109 1697 885 518 226 152 AseI AT'TA_AT Cuts at: 160 1032 4141 160 Size: 872 3109 1606 Fragments arranged by size: 3109 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 AvaI C'yCGr_G Cuts at: 1870 1943 2912 1870 Size: 73 969 4545 Fragments arranged by size: 4545 969 73 BamHI G'GATC_C Cuts at: 2917 3284 2917 Size: 367 5220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2908 4363 618 Size: 325 217 627 1121 1455 1842 Fragments arranged by size: 1842 1455 1121 627 325 217 BanII G_rGCy'C Cuts at: 1391 2906 1391 Size: 1515 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4616 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3778 3812 1903 Size: 34 1841 34 3678 Fragments arranged by size: 3678 1841 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2231 3479 4689 2231 Size: 1248 1210 3129 Fragments arranged by size: 3129 1248 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5587 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4198 136 Size: 107 122 71 631 605 2526 1525 Fragments arranged by size: 2526 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5582 5582 Size: 5587 BlpI GC'TnA_GC Cuts at: 3004 3004 Size: 5587 Bme1580I G_kGCm'C Cuts at: 1790 2536 3648 4804 1790 Size: 746 1112 1156 2573 Fragments arranged by size: 2573 1156 1112 746 BmgBI CAC'GTC Cuts at: 1318 2820 1318 Size: 1502 4085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2677 3318 4276 Size: 416 53 83 399 24 1260 641 958 Cuts at: 4276 442 Size: 1753 Fragments arranged by size: 1753 1260 958 641 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5587 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 5587 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2191 4247 1239 Size: 952 2056 2579 Fragments arranged by size: 2579 2056 952 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2010 3712 4577 4875 2010 Size: 1702 865 298 2722 Fragments arranged by size: 2722 1702 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4250 882 Size: 617 2751 2219 Fragments arranged by size: 2751 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3378 493 Size: 711 2174 2702 Fragments arranged by size: 2702 2174 711 BsaBI GATnn'nnATC Cuts at: 5581 5581 Size: 5587 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3394 3776 4411 275 Size: 53 83 186 936 1861 382 635 1451 Fragments arranged by size: 1861 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4020 4418 4692 4839 5250 4020 Size: 398 274 147 411 4357 Fragments arranged by size: 4357 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 2833 2863 1178 Size: 30 165 30 786 30 614 30 3902 Fragments arranged by size: 3902 786 614 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2640 1186 Size: 1454 4133 BseYI C'CCAG_C Cuts at: 2272 2348 4790 5286 2272 Size: 76 2442 496 2573 Fragments arranged by size: 2573 2442 496 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5014 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3037 3798 4826 1540 Size: 275 186 1036 761 1028 2301 Fragments arranged by size: 2301 1036 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2536 3648 3733 4804 2536 Size: 1112 85 1071 3319 Fragments arranged by size: 3319 1112 1071 85 BsmI GAATG_Cn' Cuts at: 2078 3098 3191 2078 Size: 1020 93 4474 Fragments arranged by size: 4474 1020 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2536 2906 3648 3733 4804 1391 Size: 399 746 370 742 85 1071 2174 Fragments arranged by size: 2174 1071 746 742 399 370 85 BspEI T'CCGG_A Cuts at: 5250 5250 Size: 5587 BspHI T'CATG_A Cuts at: 2356 2634 2781 3476 5206 2356 Size: 278 147 695 1730 2737 Fragments arranged by size: 2737 1730 695 278 147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 2942 844 Size: 1484 614 3489 Fragments arranged by size: 3489 1484 614 BsrBI CCG'CTC Cuts at: 1394 1823 3474 1394 Size: 429 1651 3507 Fragments arranged by size: 3507 1651 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4082 4256 65 Size: 1183 2834 174 1396 Fragments arranged by size: 2834 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4231 4418 1700 Size: 110 2421 187 2869 Fragments arranged by size: 2869 2421 187 110 BsrGI T'GTAC_A Cuts at: 96 2125 2822 96 Size: 2029 697 2861 Fragments arranged by size: 2861 2029 697 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5587 BssSI C'ACGA_G Cuts at: 3647 4659 3647 Size: 1012 4575 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5587 BstBI TT'CG_AA Cuts at: 4391 4391 Size: 5587 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5587 BstXI CCAn_nnnn'nTGG Cuts at: 2047 2753 2047 Size: 706 4881 BstYI r'GATC_y Cuts at: 1972 2917 3284 3669 3686 5138 5224 5236 5582 Size: 945 367 385 17 1452 86 12 346 Cuts at: 5582 1972 Size: 1977 Fragments arranged by size: 1977 1452 945 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2616 2616 Size: 5587 BtgI C'CryG_G Cuts at: 513 1065 2004 2546 513 Size: 552 939 542 3554 Fragments arranged by size: 3554 939 552 542 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4848 Fragments arranged by size: 4848 683 56 BtsI GCAGTG_nn' Cuts at: 3103 3897 3917 3103 Size: 794 20 4773 Fragments arranged by size: 4773 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5587 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5552 DraI TTT'AAA Cuts at: 2891 2891 Size: 5587 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4594 809 Size: 507 3278 1802 Fragments arranged by size: 3278 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3034 3923 5552 Size: 54 1425 50 275 18 1204 889 1629 Cuts at: 5552 8 Size: 43 Fragments arranged by size: 1629 1425 1204 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3034 1537 Size: 275 1222 4090 Fragments arranged by size: 4090 1222 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3517 1353 Size: 2164 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4174 4548 4694 387 Size: 1064 138 2585 374 146 1280 Fragments arranged by size: 2585 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 3650 4247 5034 5302 1239 Size: 393 559 1459 597 787 268 1524 Fragments arranged by size: 1524 1459 787 597 559 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2025 2652 2025 Size: 627 4960 EcoRI G'AATT_C Cuts at: 2378 2896 3274 2378 Size: 518 378 4691 Fragments arranged by size: 4691 518 378 FspI TGC'GCA Cuts at: 4093 4093 Size: 5587 HaeII r_GCGC'y Cuts at: 1678 4734 1678 Size: 3056 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2189 2221 2833 Size: 32 135 32 436 32 149 32 612 Cuts at: 2833 2865 4228 4260 4302 4334 1373 Size: 32 1363 32 42 32 2626 Fragments arranged by size: 2626 1363 612 436 149 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2137 2931 3177 669 Size: 988 281 199 794 246 3079 Fragments arranged by size: 3079 988 794 281 246 199 HindIII A'AGCT_T Cuts at: 748 748 Size: 5587 HpaI GTT'AAC Cuts at: 3177 3177 Size: 5587 KpnI G_GTAC'C Cuts at: 2912 2912 Size: 5587 MfeI C'AATT_G Cuts at: 3186 3186 Size: 5587 MluI A'CGCG_T Cuts at: 5463 5463 Size: 5587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4701 4885 1305 Size: 3396 184 2007 Fragments arranged by size: 3396 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3765 Fragments arranged by size: 3765 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2633 3545 3904 4063 518 Size: 648 879 588 912 359 159 2042 Fragments arranged by size: 2042 912 879 648 588 359 159 MspA1I CmG'CkG Cuts at: 1606 2168 3003 3680 4828 5073 1606 Size: 562 835 677 1148 245 2120 Fragments arranged by size: 2120 1148 835 677 562 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4096 Fragments arranged by size: 4096 939 552 NdeI CA'TA_TG Cuts at: 387 2130 2149 2326 387 Size: 1743 19 177 3648 Fragments arranged by size: 3648 1743 177 19 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5587 NotI GC'GGCC_GC Cuts at: 3034 3034 Size: 5587 NspI r_CATG'y Cuts at: 1132 1229 2126 2945 4490 1132 Size: 97 897 819 1545 2229 Fragments arranged by size: 2229 1545 897 819 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5587 PciI A'CATG_T Cuts at: 2122 4486 2122 Size: 2364 3223 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5587 PmeI GTTT'AAAC Cuts at: 2891 2891 Size: 5587 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2655 2687 3656 3688 5169 5201 2655 Size: 32 969 32 1481 32 3041 Fragments arranged by size: 3041 1481 969 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5587 PsiI TTA'TAA Cuts at: 3157 3157 Size: 5587 PstI C_TGCA'G Cuts at: 830 2939 830 Size: 2109 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5587 SalI G'TCGA_C Cuts at: 1936 2135 2929 1936 Size: 199 794 4594 Fragments arranged by size: 4594 794 199 SbfI CC_TGCA'GG Cuts at: 2939 2939 Size: 5587 ScaI AGT'ACT Cuts at: 3835 5547 3835 Size: 1712 3875 SfcI C'TryA_G Cuts at: 826 1045 2935 4070 4751 4942 826 Size: 219 1890 1135 681 191 1471 Fragments arranged by size: 1890 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5587 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5587 SmaI CCC'GGG Cuts at: 1872 2914 1872 Size: 1042 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2025 3691 4592 4854 820 Size: 197 926 82 1666 901 262 1553 Fragments arranged by size: 1666 1553 926 901 262 197 82 SnaBI TAC'GTA Cuts at: 493 3378 493 Size: 2885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5587 SphI G_CATG'C Cuts at: 1132 2945 1132 Size: 1813 3774 SspI AAT'ATT Cuts at: 5 52 3511 5 Size: 47 3459 2081 Fragments arranged by size: 3459 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5587 StyI C'CwwG_G Cuts at: 513 1065 2004 2537 3026 513 Size: 552 939 533 489 3074 Fragments arranged by size: 3074 939 552 533 489 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2087 2801 3614 3631 3784 3969 4414 Size: 1255 507 714 813 17 153 185 445 Cuts at: 4414 325 Size: 1498 Fragments arranged by size: 1498 1255 813 714 507 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2055 2125 2822 Size: 274 80 33 51 1443 78 70 697 Cuts at: 2822 3833 5545 96 Size: 1011 1712 138 Fragments arranged by size: 1712 1443 1011 697 274 138 80 78 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2515 2547 5547 5579 2515 Size: 32 3000 32 2523 Fragments arranged by size: 3000 2523 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5587 XbaI T'CTAG_A Cuts at: 2923 2923 Size: 5587 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2231 2753 2771 2231 Size: 522 18 5047 Fragments arranged by size: 5047 522 18 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5587 XmaI C'CCGG_G Cuts at: 1870 2912 1870 Size: 1042 4545 XmnI GAAnn'nnTTC Cuts at: 2516 3716 2516 Size: 1200 4387 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3395 276 Size: 53 83 186 936 1861 2468 Fragments arranged by size: 2468 1861 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI