Restriction map for
pFN21AE5312
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT14I FauI FbaI
FseI FspI HindIII HpaI KpnI
KspI MluI MluNI MroI MscI
MvnI NciI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AcyI 644 GGCGCC G[GA]^CG[CT]C
AhdI 43 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 212 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 43 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 644 GGCGCC G^G[CT][GA]CC
BanII 274 GAGCCC G[GA]GC[CT]^C
BbeI 644 GGCGCC GGCGC^C
BbvI 208 GCTGC GCAGC(8/12)
BglII 538 AGATCT A^GATCT
BmyI 274 GAGCCC G[AGT]GC[ACT]^C
BsaBI 533 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 644 GGCGCC G[GA]^CG[CT]C
BsaJI 426 CCACGG C^C[ACGT][ACGT]GG
BsmFI 473 GTCCC GGGAC(10/14)
Bsp1286I 274 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 177 TGTACA T^GTACA
BspHI 849 TCATGA T^CATGA
BsrFI 241 GCCGGC [GA]^CCGG[CT]
BsrGI 177 TGTACA T^GTACA
BstAPI 202 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 148 GGATG GGATG(2/0)
BstYI 538 AGATCT [GA]^GATC[CT]
CfoI 645 GCGC GCG^C
Cfr10I 241 GCCGGC [GA]^CCGG[CT]
DdeI 689 CTCAG C^T[ACGT]AG
DraII 310 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 426 CCACGG C^C[GA][CT]GG
EaeI 243 CGGCCA [CT]^GGCC[GA]
Eam1105I 43 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 772 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 310 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 256 GATATC GAT^ATC
EcoT22I 170 ATGCAT ATGCA^T
Fnu4HI 208 GCTGC GC^[ACGT]GC
FokI 148 GGATG GGATG(9/13)
HaeII 644 GGCGCC [GA]GCGC^[CT]
HapII 242 CCGG C^CGG
HgaI 471 GCGTC GACGC(5/10)
HhaI 645 GCGC GCG^C
Hin1I 644 GGCGCC G[GA]^CG[CT]C
HinP1I 645 GCGC G^CGC
HpaII 242 CCGG C^CGG
HphI 29 TCACC GGTGA(8/7)
ItaI 208 GCTGC GC^[ACGT]GC
KasI 644 GGCGCC G^GCGCC
MaeII 26 ACGT A^CGT
MamI 533 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 538 AGATCT [GA]^GATC[CT]
MseI 607 TTAA T^TAA
MslI 427 CACGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 463 CCGCTG C[AC]G^C[GT]G
MspI 242 CCGG C^CGG
NaeI 241 GCCGGC GCC^GGC
NarI 644 GGCGCC GG^CGCC
NgoMIV 241 GCCGGC G^CCGGC
NsiI 170 ATGCAT ATGCA^T
NspI 198 ACATGC [GA]CATG^[CT]
PflMI 292 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 209 CTGCAG CTGCA^G
RcaI 849 TCATGA T^CATGA
SfaNI 790 GATGC GCATC(5/9)
SfoI 644 GGCGCC GGC^GCC
SspBI 177 TGTACA T^GTACA
TfiI 337 GAATC G^A[AT]TC
Tru9I 607 TTAA T^TAA
TseI 208 GCTGC G^C[AT]GC
Van91I 292 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 538 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 178 GTAC GT^AC
183 GTAC
AlwI 723 GATCC GGATC(4/5)
532 GGATC
BfaI 640 CTAG C^TAG
715 CTAG
BpmI 218 CTGGAG CTGGAG(16/14)
649 CTGGAG
BseRI 766 GAGGAG GAGGAG(10/8)
823 GAGGAG
BsgI 320 CTGCAC GTGCAG(16/14)
452 GTGCAG
BsrI 4 ACTGG ACTGG(1/-1)
548 CCAGT
BssKI 298 CCTGG ^CC[ACGT]GG
648 CCTGG
BstNI 298 CCTGG CC^[AT]GG
648 CCTGG
Cac8I 241 GCCGGC GC[ACGT]^[ACGT]GC
771 GCCTGC
Cfr13I 311 GGCCC G^G[ACGT]CC
784 GGGCC
EarI 706 GAAGAG CTCTTC(1/4)
802 GAAGAG
EcoRII 298 CCTGG ^CC[AT]GG
648 CCTGG
HincII 106 GTTGAC GT[CT]^[GA]AC
555 GTCAAC
HindII 106 GTTGAC GT[CT]^[GA]AC
555 GTCAAC
Ksp632I 706 GAAGAG CTCTTC(1/4)
802 GAAGAG
MaeI 640 CTAG C^TAG
715 CTAG
MfeI 92 CAATTG C^AATTG
700 CAATTG
MunI 92 CAATTG C^AATTG
700 CAATTG
MvaI 298 CCTGG CC^[AT]GG
648 CCTGG
MwoI 202 GCAAGAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
208 GCTGCAGTGGC
RsaI 178 GTAC GT^AC
183 GTAC
Sau96I 311 GGCCC G^G[ACGT]CC
784 GGGCC
ScrFI 298 CCTGG CC^[ACGT]GG
648 CCTGG
TaqI 535 TCGA T^CGA
833 TCGA
Tsp45I 28 GTCAC ^GT[GC]AC
487 GTCAC
TspRI 212 CAGTG CA[GC]TG[ACGT][ACGT]^
549 CAGTG
TthHB8I 535 TCGA T^CGA
833 TCGA
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
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AciI AluI BsiYI BslI DpnII
HaeIII HinfI MaeIII MboI MboII
MnlI NdeII NlaIII NlaIV PleI
Sau3AI SfcI Tsp509I