Restriction map for
pFN21AE5312
(Circular) (Six-base) MAPSORT of: pFN21AE5312.seq Check: 821 from: 1 to: 5578
pFN21AE5312 5578 bp
With 182 enzymes: SgfI *
December 8, 2014 13:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3388 278
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2921 1937
Size: 984 4594
Acc65I G'GTAC_C
Cuts at: 2899 2899
Size: 5578
AclI AA'CG_TT
Cuts at: 1563 3705 4078 1563
Size: 2142 373 3063
Fragments arranged by size:
3063 2142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2492 2802 3641 5025 5293 1632
Size: 860 310 839 1384 268 1917
Fragments arranged by size:
1917 1384 860 839 310 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5381
AflIII A'CryG_T
Cuts at: 1203 4477 5454 1203
Size: 3274 977 1327
Fragments arranged by size:
3274 1327 977
AgeI A'CCGG_T
Cuts at: 4409 4409
Size: 5578
AhdI GACnn_n'nnGTC
Cuts at: 2053 4307 2053
Size: 2254 3324
AleI CACnn'nnGTG
Cuts at: 1166 2436 1166
Size: 1270 4308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5578
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2145 2177 2145
Size: 32 5546
AlwNI CAG_nnn'CTG
Cuts at: 2222 3321 4893 2222
Size: 1099 1572 2907
Fragments arranged by size:
2907 1572 1099
ApaLI G'TGCA_C
Cuts at: 3635 4791 3635
Size: 1156 4422
ApoI r'AATT_y
Cuts at: 1493 2887 3113 3265 5374 1493
Size: 1394 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4132 160
Size: 872 3100 1606
Fragments arranged by size:
3100 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
AvaI C'yCGr_G
Cuts at: 1870 1943 2903 1870
Size: 73 960 4545
Fragments arranged by size:
4545 960 73
BamHI G'GATC_C
Cuts at: 2908 3275 2908
Size: 367 5211
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2649 2899 4354 618
Size: 325 217 627 862 250 1455 1842
Fragments arranged by size:
1842 1455 862 627 325 250 217
BanII G_rGCy'C
Cuts at: 1391 2283 2897 1391
Size: 892 614 4072
Fragments arranged by size:
4072 892 614
BbeI G_GCGC'C
Cuts at: 2653 2653
Size: 5578
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3769 3803 1903
Size: 34 1832 34 3678
Fragments arranged by size:
3678 1832 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3470 4680 3470
Size: 1210 4368
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5578
BfrBI ATG'CAT
Cuts at: 2177 2177
Size: 5578
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4189 136
Size: 107 122 71 631 605 2517 1525
Fragments arranged by size:
2517 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2543 5573 2543
Size: 3030 2548
BlpI GC'TnA_GC
Cuts at: 2995 2995
Size: 5578
Bme1580I G_kGCm'C
Cuts at: 1790 3639 4795 1790
Size: 1849 1156 2573
Fragments arranged by size:
2573 1849 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5578
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3309 4267 442
Size: 416 53 83 399 24 1892 958 1753
Fragments arranged by size:
1892 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5578
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2244 2675 4238 1239
Size: 1005 431 1563 2579
Fragments arranged by size:
2579 1563 1005 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3703 4568 4866 3703
Size: 865 298 4415
Fragments arranged by size:
4415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4241 882
Size: 617 2742 2219
Fragments arranged by size:
2742 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3369 493
Size: 711 2165 2702
Fragments arranged by size:
2702 2165 711
BsaBI GATnn'nnATC
Cuts at: 2542 5572 2542
Size: 3030 2548
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2650 3385 3767 4402
Size: 53 83 186 936 1117 735 382 635
Cuts at: 4402 275
Size: 1451
Fragments arranged by size:
1451 1117 936 735 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4011 4409 4683 4830 5241 4011
Size: 398 274 147 411 4348
Fragments arranged by size:
4348 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5353
Fragments arranged by size:
5353 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2786 2843 1186
Size: 1600 57 3921
Fragments arranged by size:
3921 1600 57
BseYI C'CCAG_C
Cuts at: 4781 5277 4781
Size: 496 5082
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2310 2478 1163
Size: 573 574 168 4263
Fragments arranged by size:
4263 574 573 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3028 3789 4817 1540
Size: 275 186 1027 761 1028 2301
Fragments arranged by size:
2301 1028 1027 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3639 3724 4795 3639
Size: 85 1071 4422
Fragments arranged by size:
4422 1071 85
BsmI GAATG_Cn'
Cuts at: 3089 3182 3089
Size: 93 5485
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2897 3639 3724 4795 1391
Size: 399 493 614 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 614 493 399 85
BspEI T'CCGG_A
Cuts at: 5241 5241
Size: 5578
BspHI T'CATG_A
Cuts at: 2854 3467 5197 2854
Size: 613 1730 3235
Fragments arranged by size:
3235 1730 613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2933 844
Size: 2089 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3465 1394
Size: 429 1642 3507
Fragments arranged by size:
3507 1642 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4073 4247 65
Size: 1183 2825 174 1396
Fragments arranged by size:
2825 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2246 4222 4409 1700
Size: 110 436 1976 187 2869
Fragments arranged by size:
2869 1976 436 187 110
BsrGI T'GTAC_A
Cuts at: 96 2182 96
Size: 2086 3492
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5578
BssSI C'ACGA_G
Cuts at: 3638 4650 3638
Size: 1012 4566
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2213 794
Size: 1419 4159
BstBI TT'CG_AA
Cuts at: 4382 4382
Size: 5578
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5578
BstYI r'GATC_y
Cuts at: 1972 2543 2908 3275 3660 3677 5129 5215 5227
Size: 571 365 367 385 17 1452 86 12
Cuts at: 5227 5573 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 571 385 367 365 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2431 513
Size: 552 1366 3660
Fragments arranged by size:
3660 1366 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2227 487
Size: 683 56 1001 3838
Fragments arranged by size:
3838 1001 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 3094 3888 3908 2223
Size: 871 794 20 3893
Fragments arranged by size:
3893 871 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5543
DraI TTT'AAA
Cuts at: 2882 2882
Size: 5578
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4585 809
Size: 507 3269 1802
Fragments arranged by size:
3269 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 3025 3914
Size: 54 1425 50 275 18 418 777 889
Cuts at: 3914 5543 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 777 418 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3025 1537
Size: 275 1213 4090
Fragments arranged by size:
4090 1213 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2706 2802 3508 1353
Size: 1353 96 706 3423
Fragments arranged by size:
3423 1353 706 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2696 4165 4539 4685 387
Size: 1064 138 1107 1469 374 146 1280
Fragments arranged by size:
1469 1280 1107 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2492 2675 2802 3641 4238 5025
Size: 393 612 248 183 127 839 597 787
Cuts at: 5025 5293 1239
Size: 268 1524
Fragments arranged by size:
1524 839 787 612 597 393 268 248
183 127
EcoNI CCTnn'n_nnAGG
Cuts at: 2781 2781
Size: 5578
EcoO109I rG'GnC_Cy
Cuts at: 2316 2316
Size: 5578
EcoRI G'AATT_C
Cuts at: 2887 3265 2887
Size: 378 5200
EcoRV GAT'ATC
Cuts at: 2263 2263
Size: 5578
FspI TGC'GCA
Cuts at: 4084 4084
Size: 5578
HaeII r_GCGC'y
Cuts at: 1678 2653 4725 1678
Size: 975 2072 2531
Fragments arranged by size:
2531 2072 975
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2475 2507 2743
Size: 32 135 32 467 32 404 32 236
Cuts at: 2743 2775 4219 4251 4293 4325 1373
Size: 32 1444 32 42 32 2626
Fragments arranged by size:
2626 1444 467 404 236 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2113 2562 2922 3168 669
Size: 988 281 175 449 360 246 3079
Fragments arranged by size:
3079 988 449 360 281 246 175
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5578
HpaI GTT'AAC
Cuts at: 3168 3168
Size: 5578
KasI G'GCGC_C
Cuts at: 2649 2649
Size: 5578
KpnI G_GTAC'C
Cuts at: 2903 2903
Size: 5578
MfeI C'AATT_G
Cuts at: 2097 2705 3177 2097
Size: 608 472 4498
Fragments arranged by size:
4498 608 472
MluI A'CGCG_T
Cuts at: 5454 5454
Size: 5578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2131 4692 4876 1305
Size: 826 2561 184 2007
Fragments arranged by size:
2561 2007 826 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3756
Fragments arranged by size:
3756 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2436 3536 3895 4054 518
Size: 648 1270 1100 359 159 2042
Fragments arranged by size:
2042 1270 1100 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2470 2994 3671 4819 5064 1606
Size: 864 524 677 1148 245 2120
Fragments arranged by size:
2120 1148 864 677 524 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 5578
NarI GG'CG_CC
Cuts at: 2650 2650
Size: 5578
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5026
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5578
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5578
NotI GC'GGCC_GC
Cuts at: 3025 3025
Size: 5578
NsiI A_TGCA'T
Cuts at: 2179 2179
Size: 5578
NspI r_CATG'y
Cuts at: 1132 1229 2207 2936 4481 1132
Size: 97 978 729 1545 2229
Fragments arranged by size:
2229 1545 978 729 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5578
PciI A'CATG_T
Cuts at: 4477 4477
Size: 5578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2303 1108
Size: 1195 4383
PmeI GTTT'AAAC
Cuts at: 2882 2882
Size: 5578
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 5160 5192 3647
Size: 32 1481 32 4033
Fragments arranged by size:
4033 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5578
PsiI TTA'TAA
Cuts at: 3148 3148
Size: 5578
PstI C_TGCA'G
Cuts at: 830 2218 2930 830
Size: 1388 712 3478
Fragments arranged by size:
3478 1388 712
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5578
SalI G'TCGA_C
Cuts at: 1936 2920 1936
Size: 984 4594
SbfI CC_TGCA'GG
Cuts at: 2930 2930
Size: 5578
ScaI AGT'ACT
Cuts at: 3826 5538 3826
Size: 1712 3866
SfcI C'TryA_G
Cuts at: 826 1045 2214 2395 2766 2867 2926 4061 4742
Size: 219 1169 181 371 101 59 1135 681
Cuts at: 4742 4933 826
Size: 191 1471
Fragments arranged by size:
1471 1169 1135 681 371 219 191 181
101 59
SfoI GGC'GCC
Cuts at: 2651 2651
Size: 5578
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5578
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5578
SmaI CCC'GGG
Cuts at: 1872 2905 1872
Size: 1033 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3682 4583 4845 820
Size: 197 926 1739 901 262 1553
Fragments arranged by size:
1739 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3369 493
Size: 2876 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5578
SphI G_CATG'C
Cuts at: 1132 2936 1132
Size: 1804 3774
SspI AAT'ATT
Cuts at: 5 52 3502 5
Size: 47 3450 2081
Fragments arranged by size:
3450 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5578
StyI C'CwwG_G
Cuts at: 513 1065 3017 513
Size: 552 1952 3074
Fragments arranged by size:
3074 1952 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3605 3622 3775 3960 4405 325
Size: 1255 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2182 2187 3824
Size: 274 80 33 51 1443 205 5 1637
Cuts at: 3824 5536 96
Size: 1712 138
Fragments arranged by size:
1712 1637 1443 274 205 138 80 51
33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5538 5570 5538
Size: 32 5546
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5578
XbaI T'CTAG_A
Cuts at: 2914 2914
Size: 5578
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5578
XmaI C'CCGG_G
Cuts at: 1870 2903 1870
Size: 1033 4545
XmnI GAAnn'nnTTC
Cuts at: 3707 3707
Size: 5578
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3386 276
Size: 53 83 186 936 1852 2468
Fragments arranged by size:
2468 1852 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI