(Linear) (Six-base) MAPSORT of: KIEE5312.seq Check: 9750 from: 1 to: 873 KIEE5312.orf With 182 enzymes: SgfI * December 5, 2014 13:00 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 487 797 873 Size: 487 310 76 Fragments arranged by size: 487 310 76 AhdI GACnn_n'nnGTC Cuts at: 0 48 873 Size: 48 825 AleI CACnn'nnGTG Cuts at: 0 431 873 Size: 431 442 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 140 172 873 Size: 140 32 701 Fragments arranged by size: 701 140 32 AlwNI CAG_nnn'CTG Cuts at: 0 217 873 Size: 217 656 BanI G'GyrC_C Cuts at: 0 644 873 Size: 644 229 BanII G_rGCy'C Cuts at: 0 278 873 Size: 278 595 BbeI G_GCGC'C Cuts at: 0 648 873 Size: 648 225 BfrBI ATG'CAT Cuts at: 0 172 873 Size: 172 701 BglII A'GATC_T Cuts at: 0 538 873 Size: 538 335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 670 873 Size: 239 431 203 Fragments arranged by size: 431 239 203 BsaBI GATnn'nnATC Cuts at: 0 537 873 Size: 537 336 BsaHI Gr'CG_yC Cuts at: 0 645 873 Size: 645 228 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 781 838 873 Size: 781 57 35 Fragments arranged by size: 781 57 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 473 873 Size: 305 168 400 Fragments arranged by size: 400 305 168 Bsp1286I G_dGCh'C Cuts at: 0 278 873 Size: 278 595 BspHI T'CATG_A Cuts at: 0 849 873 Size: 849 24 BsrFI r'CCGG_y Cuts at: 0 241 873 Size: 241 632 BsrGI T'GTAC_A Cuts at: 0 177 873 Size: 177 696 BstAPI GCAn_nnn'nTGC Cuts at: 0 208 873 Size: 208 665 BstYI r'GATC_y Cuts at: 0 538 873 Size: 538 335 BtgI C'CryG_G Cuts at: 0 426 873 Size: 426 447 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 222 873 Size: 222 651 BtsI GCAGTG_nn' Cuts at: 0 218 873 Size: 218 655 EaeI y'GGCC_r Cuts at: 0 243 873 Size: 243 630 EarI CTCTTCn'nnn_ Cuts at: 0 701 797 873 Size: 701 96 76 Fragments arranged by size: 701 96 76 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 691 873 Size: 691 182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 487 670 797 873 Size: 239 248 183 127 76 Fragments arranged by size: 248 239 183 127 76 EcoNI CCTnn'n_nnAGG Cuts at: 0 776 873 Size: 776 97 EcoO109I rG'GnC_Cy Cuts at: 0 311 873 Size: 311 562 EcoRV GAT'ATC Cuts at: 0 258 873 Size: 258 615 HaeII r_GCGC'y Cuts at: 0 648 873 Size: 648 225 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 34 66 470 502 738 770 873 Size: 34 32 404 32 236 32 103 Fragments arranged by size: 404 236 103 34 32 32 32 HincII GTy'rAC Cuts at: 0 108 557 873 Size: 108 449 316 Fragments arranged by size: 449 316 108 KasI G'GCGC_C Cuts at: 0 644 873 Size: 644 229 MfeI C'AATT_G Cuts at: 0 92 700 873 Size: 92 608 173 Fragments arranged by size: 608 173 92 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 126 873 Size: 126 747 MslI CAynn'nnrTG Cuts at: 0 431 873 Size: 431 442 MspA1I CmG'CkG Cuts at: 0 465 873 Size: 465 408 NaeI GCC'GGC Cuts at: 0 243 873 Size: 243 630 NarI GG'CG_CC Cuts at: 0 645 873 Size: 645 228 NgoMIV G'CCGG_C Cuts at: 0 241 873 Size: 241 632 NsiI A_TGCA'T Cuts at: 0 174 873 Size: 174 699 NspI r_CATG'y Cuts at: 0 202 873 Size: 202 671 PflMI CCAn_nnn'nTGG Cuts at: 0 298 873 Size: 298 575 PstI C_TGCA'G Cuts at: 0 213 873 Size: 213 660 SfcI C'TryA_G Cuts at: 0 209 390 761 862 873 Size: 209 181 371 101 11 Fragments arranged by size: 371 209 181 101 11 SfoI GGC'GCC Cuts at: 0 646 873 Size: 646 227 TatI w'GTAC_w Cuts at: 0 177 182 873 Size: 177 5 691 Fragments arranged by size: 691 177 5 Enzymes that do cut: AcuI AhdI AleI AloI AlwNI BanI BanII BbeI BfrBI BglII BpmI BsaBI BsaHI BseRI BsgI Bsp1286I BspHI BsrFI BsrGI BstAPI BstYI BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI MfeI MmeI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PflMI PstI SfcI SfoI TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI FalI FseI FspI FspAI HindIII HpaI KpnI MluI MscI NcoI NdeI NheI NotI NruI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI